CatLogenzyme kinetics catalog

Values CatLog read from this paper

Two distinct domains of the beta subunit of Aquifex aeolicus leucyl-tRNA synthetase are involved in tRNA binding as revealed by a three-hybrid selection

PMID 15208367doi:10.1093/nar/gkh665Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 26 records (39 values) from this paper: αβ-LeuRS (leucyl-tRNA synthetase) from Aquifex aeolicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.1.1.4

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
αβ-LeuRS (leucyl-tRNA synthetase)Aquifex aeolicusA. aeolicus tRNALeu0.322 × 10⁻⁴1.6 × 10³ calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)Aquifex aeolicusL-leucine3.9——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant A156VAquifex aeolicusA. aeolicus tRNALeu0.845.1 × 10⁻⁴1.6 × 10³ calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant A156VAquifex aeolicusL-leucine4.1——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant G237DAquifex aeolicusA. aeolicus tRNALeu0.391.4 × 10⁻⁴2.8 × 10³ calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant G237DAquifex aeolicusL-leucine4——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant K160NAquifex aeolicusA. aeolicus tRNALeu0.662.4 × 10⁻⁴2.8 × 10³ calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant K160NAquifex aeolicusL-leucine3.9——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant K238AAquifex aeolicusA. aeolicus tRNALeu0.421.1 × 10⁻⁴3.8 × 10³ calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant K238AAquifex aeolicusL-leucine4.2——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant L283FAquifex aeolicusA. aeolicus tRNALeu0.591.8 × 10⁻⁴3.3 × 10³ calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant L283FAquifex aeolicusL-leucine4.5——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant M159AAquifex aeolicusA. aeolicus tRNALeu0.473.8 × 10⁻⁴1.2 × 10³ calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant M159AAquifex aeolicusL-leucine4.1——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant N152AAquifex aeolicusA. aeolicus tRNALeu0.0722.9 × 10⁻⁴250 calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant N152AAquifex aeolicusL-leucine3.8——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant N163AAquifex aeolicusA. aeolicus tRNALeu0.391 × 10⁻⁴3.9 × 10³ calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant N163AAquifex aeolicusL-leucine4.3——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant Q234HAquifex aeolicusA. aeolicus tRNALeu0.42.5 × 10⁻⁴1.6 × 10³ calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant Q234HAquifex aeolicusL-leucine3.7——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant Q269stopAquifex aeolicusA. aeolicus tRNALeu0.0030.00950.32 calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant Q269stopAquifex aeolicusL-leucine4.1——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant R94AAquifex aeolicusA. aeolicus tRNALeu0.315 × 10⁻⁴620 calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant R94AAquifex aeolicusL-leucine4.1——37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant V286stopAquifex aeolicusA. aeolicus tRNALeu0.320.0359.1 calc.pH 7.8, 37 °COpen
αβ-LeuRS (leucyl-tRNA synthetase)variant V286stopAquifex aeolicusL-leucine4.1——37 °COpen