CatLogenzyme kinetics catalog

EC 6.2.1.45: E1 ubiquitin-activating enzyme

LigasesOpen in Browse

CatLog read 22 records for EC 6.2.1.45 from 1 paper, for 1 organism. Names in these papers: ubiquitin-activating enzyme (HsUba1a, human nuclear form).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 3 records for EC 6.2.1.45 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.2.1.45 in Browse.

Papers

PaperOrganismsRecords
Pleiotropic effects of ATP.Mg2+ binding in the catalytic cycle of ubiquitin-activating enzymeHomo sapiens22

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
ubiquitin-activating enzyme (HsUba1a, human nuclear form)Homo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)—0.0055—pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)Homo sapienshuman ubiquitin-conjugating enzyme Ubc2b (E2-14Kb, ortholog of yeast Rad6/Ubc2)3.41.11 × 10⁻⁴3.1 × 10⁴ calc.pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)Homo sapiensubiquitin (bovine)6——pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)Homo sapiensubiquitin (bovine, radioiodinated with 125I)—8 × 10⁻⁴—pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)direct GST-HsUba1a-125I-ubiquitin thiolester formation assay—0.208—pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)direct GST-HsUba1a-125I-ubiquitin thiolester formation assay0.0035——pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)catalytic specificity with respect to ATP·Mg2+; in the text——0.115pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapienshuman ubiquitin-conjugating enzyme Ubc2b (E2-14Kb, ortholog of yeast Rad6/Ubc2)0.121.35 × 10⁻⁴890 calc.pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapiensubiquitin (bovine)0.7——pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapiensubiquitin (bovine, radioiodinated with 125I)0.0240.0290.83 calc.pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576EHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)ATP varied (Fig. 3 and parallel experiments)—0.022—pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576EHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)ATP varied (parallel to Fig. 3); 5 µM 125I-ubiquitin0.006——pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576EHomo sapienshuman ubiquitin-conjugating enzyme Ubc2b (E2-14Kb, ortholog of yeast Rad6/Ubc2)0.11.22 × 10⁻⁴820 calc.pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576EHomo sapiensubiquitin (bovine)1.3——pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576EHomo sapiensubiquitin (bovine, radioiodinated with 125I)0.0050.00114.5 calc.pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576NHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)ATP varied (Fig. 3 and parallel experiments)—0.029—pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576NHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)ATP varied (parallel to Fig. 3); 5 µM 125I-ubiquitin0.005——pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576NHomo sapienshuman ubiquitin-conjugating enzyme Ubc2b (E2-14Kb, ortholog of yeast Rad6/Ubc2)0.0227.3 × 10⁻⁵300 calc.pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576NHomo sapiensubiquitin (bovine, radioiodinated with 125I)0.0110.0042.8 calc.pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant K528AHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)—0.119—pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant K528AHomo sapienshuman ubiquitin-conjugating enzyme Ubc2b (E2-14Kb, ortholog of yeast Rad6/Ubc2)0.0118.9 × 10⁻⁵120 calc.pH 7.5, 37 °CPMID 16595681Open
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant K528AHomo sapiensubiquitin (bovine, radioiodinated with 125I)0.0150.00236.5 calc.pH 7.5, 37 °CPMID 16595681Open