CatLogenzyme kinetics catalog

Values CatLog read from this paper

Pleiotropic effects of ATP.Mg2+ binding in the catalytic cycle of ubiquitin-activating enzyme

PMID 16595681doi:10.1074/jbc.m513562200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (31 values) from this paper: ubiquitin-activating enzyme (HsUba1a, human nuclear form) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.2.1.45

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
ubiquitin-activating enzyme (HsUba1a, human nuclear form)Homo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)—0.0055—pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)Homo sapienshuman ubiquitin-conjugating enzyme Ubc2b (E2-14Kb, ortholog of yeast Rad6/Ubc2)3.41.11 × 10⁻⁴3.1 × 10⁴ calc.pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)Homo sapiensubiquitin (bovine)6——pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)Homo sapiensubiquitin (bovine, radioiodinated with 125I)—8 × 10⁻⁴—pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)direct GST-HsUba1a-125I-ubiquitin thiolester formation assay—0.208—pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)direct GST-HsUba1a-125I-ubiquitin thiolester formation assay0.0035——pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)catalytic specificity with respect to ATP·Mg2+; in the text——0.115pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapienshuman ubiquitin-conjugating enzyme Ubc2b (E2-14Kb, ortholog of yeast Rad6/Ubc2)0.121.35 × 10⁻⁴890 calc.pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapiensubiquitin (bovine)0.7——pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576AHomo sapiensubiquitin (bovine, radioiodinated with 125I)0.0240.0290.83 calc.pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576EHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)ATP varied (Fig. 3 and parallel experiments)—0.022—pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576EHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)ATP varied (parallel to Fig. 3); 5 µM 125I-ubiquitin0.006——pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576EHomo sapienshuman ubiquitin-conjugating enzyme Ubc2b (E2-14Kb, ortholog of yeast Rad6/Ubc2)0.11.22 × 10⁻⁴820 calc.pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576EHomo sapiensubiquitin (bovine)1.3——pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576EHomo sapiensubiquitin (bovine, radioiodinated with 125I)0.0050.00114.5 calc.pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576NHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)ATP varied (Fig. 3 and parallel experiments)—0.029—pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576NHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)ATP varied (parallel to Fig. 3); 5 µM 125I-ubiquitin0.005——pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576NHomo sapienshuman ubiquitin-conjugating enzyme Ubc2b (E2-14Kb, ortholog of yeast Rad6/Ubc2)0.0227.3 × 10⁻⁵300 calc.pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant D576NHomo sapiensubiquitin (bovine, radioiodinated with 125I)0.0110.0042.8 calc.pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant K528AHomo sapiensadenosine 5'-triphosphate (as ATP·Mg2+)—0.119—pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant K528AHomo sapienshuman ubiquitin-conjugating enzyme Ubc2b (E2-14Kb, ortholog of yeast Rad6/Ubc2)0.0118.9 × 10⁻⁵120 calc.pH 7.5, 37 °COpen
ubiquitin-activating enzyme (HsUba1a, human nuclear form)variant K528AHomo sapiensubiquitin (bovine, radioiodinated with 125I)0.0150.00236.5 calc.pH 7.5, 37 °COpen