EC 6.3.2.28: L-amino-acid alpha-ligase

CatLog read 14 records for EC 6.3.2.28 from 1 paper, for 1 organism. Names in these papers: BacD.

EC 6.3.2.28 is no longer current; BRENDA: “transferred to EC 6.3.2.49”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

Papers with records for EC 6.3.2.28
Paper	Organisms	Records
Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis	Bacillus subtilis	14

Records

Records for EC 6.3.2.28 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
BacD	Bacillus subtilis	L-alaninecleaved off with TEV protease	—	1.7	—	pH 7.7, 37 °C	PMID 22407814	Open record: BacD, L-alanine
BacD	Bacillus subtilis	L-alaninetag not cleaved off	—	2	—	pH 7.7, 37 °C	PMID 22407814	Open record: BacD, L-alanine
BacD	Bacillus subtilis	L-alanine	—	2.6	—	pH 7.1, 37 °C	PMID 22407814	Open record: BacD, L-alanine
BacD	Bacillus subtilis	L-phenylalaninecleaved off with TEV protease	10.5	20	0.53 calc.	pH 7.7, 37 °C	PMID 22407814	Open record: BacD, L-phenylalanine
BacD	Bacillus subtilis	L-phenylalaninetag not cleaved off	9.67	18	0.54 calc.	pH 7.7, 37 °C	PMID 22407814	Open record: BacD, L-phenylalanine
BacD	Bacillus subtilis	L-phenylalanine	7.67	44	0.17 calc.	pH 7.1, 37 °C	PMID 22407814	Open record: BacD, L-phenylalanine
BacDvariant S184A	Bacillus subtilis	L-alanine	—	4.6	—	pH 7.7, 37 °C	PMID 22407814	Open record: BacD variant S184A, L-alanine
BacDvariant S184A	Bacillus subtilis	L-alanine	—	4.9	—	pH 7.1, 37 °C	PMID 22407814	Open record: BacD variant S184A, L-alanine
BacDvariant S184A	Bacillus subtilis	L-phenylalanine	10.83	16	0.68 calc.	pH 7.7, 37 °C	PMID 22407814	Open record: BacD variant S184A, L-phenylalanine
BacDvariant S184A	Bacillus subtilis	L-phenylalanine	7.83	38	0.21 calc.	pH 7.1, 37 °C	PMID 22407814	Open record: BacD variant S184A, L-phenylalanine
BacDvariant Y75F	Bacillus subtilis	L-alanine	—	1.7	—	pH 7.7, 37 °C	PMID 22407814	Open record: BacD variant Y75F, L-alanine
BacDvariant Y75F	Bacillus subtilis	L-alanine	—	1.5	—	pH 7.1, 37 °C	PMID 22407814	Open record: BacD variant Y75F, L-alanine
BacDvariant Y75F	Bacillus subtilis	L-phenylalanine	9.67	45	0.21 calc.	pH 7.7, 37 °C	PMID 22407814	Open record: BacD variant Y75F, L-phenylalanine
BacDvariant Y75F	Bacillus subtilis	L-phenylalanine	7.17	92	0.078 calc.	pH 7.1, 37 °C	PMID 22407814	Open record: BacD variant Y75F, L-phenylalanine