Values CatLog read from this paper
Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis
PMID 22407814doi:10.1002/pro.2058Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (21 values) from this paper: BacD from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| BacD | Bacillus subtilis | L-alaninecleaved off with TEV protease | — | 1.7 | — | pH 7.7, 37 °C | Open record: BacD, L-alanine |
| BacD | Bacillus subtilis | L-alaninetag not cleaved off | — | 2 | — | pH 7.7, 37 °C | Open record: BacD, L-alanine |
| BacD | Bacillus subtilis | L-alanine | — | 2.6 | — | pH 7.1, 37 °C | Open record: BacD, L-alanine |
| BacD | Bacillus subtilis | L-phenylalaninecleaved off with TEV protease | 10.5 | 20 | 0.53 calc. | pH 7.7, 37 °C | Open record: BacD, L-phenylalanine |
| BacD | Bacillus subtilis | L-phenylalaninetag not cleaved off | 9.67 | 18 | 0.54 calc. | pH 7.7, 37 °C | Open record: BacD, L-phenylalanine |
| BacD | Bacillus subtilis | L-phenylalanine | 7.67 | 44 | 0.17 calc. | pH 7.1, 37 °C | Open record: BacD, L-phenylalanine |
| BacDvariant S184A | Bacillus subtilis | L-alanine | — | 4.6 | — | pH 7.7, 37 °C | Open record: BacD variant S184A, L-alanine |
| BacDvariant S184A | Bacillus subtilis | L-alanine | — | 4.9 | — | pH 7.1, 37 °C | Open record: BacD variant S184A, L-alanine |
| BacDvariant S184A | Bacillus subtilis | L-phenylalanine | 10.83 | 16 | 0.68 calc. | pH 7.7, 37 °C | Open record: BacD variant S184A, L-phenylalanine |
| BacDvariant S184A | Bacillus subtilis | L-phenylalanine | 7.83 | 38 | 0.21 calc. | pH 7.1, 37 °C | Open record: BacD variant S184A, L-phenylalanine |
| BacDvariant Y75F | Bacillus subtilis | L-alanine | — | 1.7 | — | pH 7.7, 37 °C | Open record: BacD variant Y75F, L-alanine |
| BacDvariant Y75F | Bacillus subtilis | L-alanine | — | 1.5 | — | pH 7.1, 37 °C | Open record: BacD variant Y75F, L-alanine |
| BacDvariant Y75F | Bacillus subtilis | L-phenylalanine | 9.67 | 45 | 0.21 calc. | pH 7.7, 37 °C | Open record: BacD variant Y75F, L-phenylalanine |
| BacDvariant Y75F | Bacillus subtilis | L-phenylalanine | 7.17 | 92 | 0.078 calc. | pH 7.1, 37 °C | Open record: BacD variant Y75F, L-phenylalanine |