EC 6.3.2.4: D-Alanine-D-alanine ligase
LigasesOpen in Browse
CatLog read 54 records for EC 6.3.2.4 from 2 papers, for 2 organisms. Names in these papers: D-Ala-D-Ala ligase B (DdlB), UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 58 records for EC 6.3.2.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.2.4 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Gain of D-alanyl-D-lactate or D-lactyl-D-alanine synthetase activities in three active-site mutants of the Escherichia coli D-alanyl-D-alanine ligase B | Escherichia coli | 32 |
| ENZYMATIC SYNTHESIS OF ANALOGS OF THE CELL-WALL PRECURSOR. I. KINETICS AND SPECIFICITY OF URIDINE DIPHOSPHO-N-ACETYLMURAMYL-L-ALANYL-D-GLUTAMYL-L-LYSINE:D-ALANYL-D-ALANINE LIGASE (ADENOSINE… | Streptococcus faecalis | 22 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| D-Ala-D-Ala ligase B (DdlB) | Escherichia coli | D-alanine | — | 0.11 | — | pH 6, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB), D-alanine |
| D-Ala-D-Ala ligase B (DdlB) | Escherichia coli | D-alanine | 11.8 | 8 | 1.48 | pH 6, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB), D-alanine |
| D-Ala-D-Ala ligase B (DdlB) | Escherichia coli | D-alanine | — | 0.003 | — | pH 7.5, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB), D-alanine |
| D-Ala-D-Ala ligase B (DdlB) | Escherichia coli | D-alanine | 14.7 | 2 | 7.3 | pH 7.5, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB), D-alanine |
| D-Ala-D-Ala ligase B (DdlB) | Escherichia coli | D-alanine | — | 0.004 | — | pH 9.2, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB), D-alanine |
| D-Ala-D-Ala ligase B (DdlB) | Escherichia coli | D-alanine | 27 | 1.1 | 25 | pH 9.2, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB), D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant E15Q | Escherichia coli | D-alanine | — | 11 | — | pH 6, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant E15Q, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant E15Q | Escherichia coli | D-alanine | 3.3 | 82 | 0.04 | pH 6, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant E15Q, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant E15Q | Escherichia coli | D-alanine | — | 0.51 | — | pH 7.5, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant E15Q, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant E15Q | Escherichia coli | D-alanine | 13.7 | 74 | 0.18 | pH 7.5, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant E15Q, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant E15Q | Escherichia coli | D-alanine | — | 0.51 | — | pH 9.2, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant E15Q, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant E15Q | Escherichia coli | D-alanine | 18 | 65 | 0.28 | pH 9.2, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant E15Q, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant E15Q | Escherichia coli | D-lactate | — | — | 1.7 × 10⁻⁴ | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant E15Q, D-lactate | |
| D-Ala-D-Ala ligase B (DdlB)variant S150A | Escherichia coli | D-2-hydroxybutyrate | 0.018 | 5.3 | 0.0035 | pH 6 | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant S150A, D-2-hydroxybutyrate |
| D-Ala-D-Ala ligase B (DdlB)variant S150A | Escherichia coli | D-alanine | — | 6.2 | — | pH 6, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant S150A, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant S150A | Escherichia coli | D-alanine | 3.3 | 140 | 0.023 | pH 6, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant S150A, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant S150A | Escherichia coli | D-alanine | — | 0.85 | — | pH 7.5, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant S150A, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant S150A | Escherichia coli | D-alanine | 16.3 | 80 | 0.2 | pH 7.5, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant S150A, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant S150A | Escherichia coli | D-alanine | — | 0.26 | — | pH 9.2, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant S150A, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant S150A | Escherichia coli | D-alanine | 18 | 44 | 0.43 | pH 9.2, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant S150A, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant S150A | Escherichia coli | D-lactate | 0.023 | 1.6 | 0.0145 | pH 6, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant S150A, D-lactate |
| D-Ala-D-Ala ligase B (DdlB)variant S150A | Escherichia coli | D-lactate | 0.057 | 2.2 | 0.025 | pH 7.5, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant S150A, D-lactate |
| D-Ala-D-Ala ligase B (DdlB)variant Y216F | Escherichia coli | D-2-hydroxybutyrate | 0.088 | 8.9 | 0.01 | pH 6 | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant Y216F, D-2-hydroxybutyrate |
| D-Ala-D-Ala ligase B (DdlB)variant Y216F | Escherichia coli | D-2-hydroxybutyrate | 0.103 | 40 | 0.0027 | pH 7.5 | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant Y216F, D-2-hydroxybutyrate |
| D-Ala-D-Ala ligase B (DdlB)variant Y216F | Escherichia coli | D-alanine | — | 0.26 | — | pH 6, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant Y216F, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant Y216F | Escherichia coli | D-alanine | 7.5 | 150 | 0.05 | pH 6, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant Y216F, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant Y216F | Escherichia coli | D-alanine | — | 0.33 | — | pH 7.5, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant Y216F, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant Y216F | Escherichia coli | D-alanine | 10.5 | 16 | 0.65 | pH 7.5, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant Y216F, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant Y216F | Escherichia coli | D-alanine | — | 0.17 | — | pH 9.2, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant Y216F, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant Y216F | Escherichia coli | D-alanine | 20 | 4.8 | 4.3 | pH 9.2, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant Y216F, D-alanine |
| D-Ala-D-Ala ligase B (DdlB)variant Y216F | Escherichia coli | D-lactate | 0.13 | 5.1 | 0.025 | pH 6, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant Y216F, D-lactate |
| D-Ala-D-Ala ligase B (DdlB)variant Y216F | Escherichia coli | D-lactate | 0.7 | 27 | 0.027 | pH 7.5, 37 °C | PMID 8756703 | Open record: D-Ala-D-Ala ligase B (DdlB) variant Y216F, D-lactate |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | ATP | — | 0.096 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), ATP |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-alanineLineweaver-Burk plot (Figure 5); Table IV | — | 0.16 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-alanine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-alanineDixon plot (Figure 8); Table IV | — | 0.16 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-alanine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-alanineKmi' calculated with equation (6); Table IV | — | 0.11 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-alanine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-alanineKmi' calculated with equation (6); Table IV | — | 0.14 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-alanine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-alaninereciprocal plots (Figure 6a); in the text | — | 0.16 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-alanine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-alpha-amino-n-butyric acidLineweaver-Burk plot (Figure 5) | — | 0.76 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-alpha-amino-n-butyric acid |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-alpha-amino-n-butyric acidDixon plot; UDP-NAc-muramyl-(Ala, Glu, Lys); MgATP2⁻ | — | 0.88 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-alpha-amino-n-butyric acid |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-alpha-amino-n-butyric acidKmi' calculated with equation (6) | — | 0.89 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-alpha-amino-n-butyric acid |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-serineLineweaver-Burk plot (Figure 5) | — | 0.91 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-serine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-serineDixon plot; UDP-NAc-muramyl-(Ala, Glu, Lys); MgATP2⁻ | — | 0.9 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-serine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-serineKmi' calculated with equation (6) | — | 0.91 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-serine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-threonineLineweaver-Burk plot (Figure 5) | — | 3.3 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-threonine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-threonineDixon plot; UDP-NAc-muramyl-(Ala, Glu, Lys); MgATP2⁻ | — | 2.7 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-threonine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-threonineKmi' calculated with equation (6) | — | 3.6 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-threonine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alanyl-D-valine | — | 2.6 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alanyl-D-valine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alpha-amino-n-butyryl-D-alanine | — | 0.09 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alpha-amino-n-butyryl-D-alanine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-alpha-amino-n-butyryl-D-alpha-amino-n-butyric acid | — | 0.7 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-alpha-amino-n-butyryl-D-alpha-amino-n-butyric acid |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-norvalyl-D-alanine | — | 0.12 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-norvalyl-D-alanine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-seryl-D-alanine | — | 0.26 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-seryl-D-alanine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | D-valyl-D-alanine | — | 0.39 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), D-valyl-D-alanine |
| UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme) | Streptococcus faecalis | uridine diphospho-N-acetylmuramyl-L-alanyl-D-glutamyl-L-lysine | — | 0.08 | — | pH 7.6, 37 °C | PMID 14285229 | Open record: UDP-NAc-muramyl-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase (ADP) (D-Ala-D-Ala adding enzyme), uridine diphospho-N-acetylmuramyl-L-alanyl-D-glutamyl-L-lysine |