CatLogenzyme kinetics catalog

Values CatLog read from this paper

Gain of D-alanyl-D-lactate or D-lactyl-D-alanine synthetase activities in three active-site mutants of the Escherichia coli D-alanyl-D-alanine ligase B

PMID 8756703doi:10.1021/bi9603128Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 50 records (105 values) from this paper: D-Ala-D-Ala ligase B (DdlB), VanA (D-Ala-D-Lac ligase) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.3.2.4

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
D-Ala-D-Ala ligase B (DdlB)Escherichia coliD-alanine—0.11—pH 6, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)Escherichia coliD-alanine11.881.48pH 6, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)Escherichia coliD-alanine—0.003—pH 7.5, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)Escherichia coliD-alanine14.727.3pH 7.5, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)Escherichia coliD-alanine—0.004—pH 9.2, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)Escherichia coliD-alanine271.125pH 9.2, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant E15QEscherichia coliD-alanine—11—pH 6, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant E15QEscherichia coliD-alanine3.3820.04pH 6, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant E15QEscherichia coliD-alanine—0.51—pH 7.5, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant E15QEscherichia coliD-alanine13.7740.18pH 7.5, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant E15QEscherichia coliD-alanine—0.51—pH 9.2, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant E15QEscherichia coliD-alanine18650.28pH 9.2, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant E15QEscherichia coliD-lactate——1.7 × 10⁻⁴Open
D-Ala-D-Ala ligase B (DdlB)variant S150AEscherichia coliD-2-hydroxybutyrate0.0185.30.0035pH 6Open
D-Ala-D-Ala ligase B (DdlB)variant S150AEscherichia coliD-alanine—6.2—pH 6, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant S150AEscherichia coliD-alanine3.31400.023pH 6, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant S150AEscherichia coliD-alanine—0.85—pH 7.5, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant S150AEscherichia coliD-alanine16.3800.2pH 7.5, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant S150AEscherichia coliD-alanine—0.26—pH 9.2, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant S150AEscherichia coliD-alanine18440.43pH 9.2, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant S150AEscherichia coliD-lactate0.0231.60.0145pH 6, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant S150AEscherichia coliD-lactate0.0572.20.025pH 7.5, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant Y216FEscherichia coliD-2-hydroxybutyrate0.0888.90.01pH 6Open
D-Ala-D-Ala ligase B (DdlB)variant Y216FEscherichia coliD-2-hydroxybutyrate0.103400.0027pH 7.5Open
D-Ala-D-Ala ligase B (DdlB)variant Y216FEscherichia coliD-alanine—0.26—pH 6, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant Y216FEscherichia coliD-alanine7.51500.05pH 6, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant Y216FEscherichia coliD-alanine—0.33—pH 7.5, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant Y216FEscherichia coliD-alanine10.5160.65pH 7.5, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant Y216FEscherichia coliD-alanine—0.17—pH 9.2, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant Y216FEscherichia coliD-alanine204.84.3pH 9.2, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant Y216FEscherichia coliD-lactate0.135.10.025pH 6, 37 °COpen
D-Ala-D-Ala ligase B (DdlB)variant Y216FEscherichia coliD-lactate0.7270.027pH 7.5, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine——0.0023pH 6.4, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanineD-Ala-D-Ala ligase activity; cosubstrate ATP—0.1—pH 7.5, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanineD-Ala-D-Ala ligase activity; cosubstrate ATP15.7—0.028pH 7.5, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine—0.37—pH 7.9, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine15.72600.06pH 7.9, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine—0.31—pH 8.3, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine15690.22pH 8.3, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine—0.67—pH 8.6, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine17740.23pH 8.6, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine—0.21—pH 9.2, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine18470.4pH 9.2, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine—4.9—pH 6, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanine—1.9—pH 6.5, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-alanineD-Ala-D-Lac ligase activity; cosubstrate D-Lac, ATP—0.23—pH 7.5, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-lactate0.651.30.5pH 6, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-lactate0.671.10.63pH 6.5, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-lactate0.750.880.85pH 7.5, 37 °COpen
VanA (D-Ala-D-Lac ligase)—D-lactate1.121.50.75pH 8.3, 37 °COpen