EC 6.3.3.6: carbapenam-3-carboxylate synthase
LigasesOpen in Browse
CatLog read 20 records for EC 6.3.3.6 from 2 papers, for 2 organisms. Names in these papers: carbapenam synthetase (CarA), carbapenam synthetase (CPS).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Inhibition and alternate substrate studies on the mechanism of carbapenam synthetase from Erwinia carotovora | Erwinia carotovora | 12 |
| A conserved tyrosyl-glutamyl catalytic dyad in evolutionarily linked enzymes: carbapenam synthetase and beta-lactam synthetase | Pectobacterium carotovorum | 8 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| carbapenam synthetase (CarA) | Erwinia carotovora | (2R,5R)-5-carboxymethyl proline | 0.122 | 4.6 | 0.026 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), (2R,5R)-5-carboxymethyl proline |
| carbapenam synthetase (CarA) | Erwinia carotovora | (2S,5R)-5-carboxymethyl proline | 0.2 | 7 | 0.027 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), (2S,5R)-5-carboxymethyl proline |
| carbapenam synthetase (CarA) | Erwinia carotovora | (2S,5S)-5-carboxyethyl proline | 0.46 | 3.4 | 0.13 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), (2S,5S)-5-carboxyethyl proline |
| carbapenam synthetase (CarA) | Erwinia carotovora | (2S,5S)-5-carboxymethyl proline | 0.28 | 0.23 | 1.2 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), (2S,5S)-5-carboxymethyl proline |
| carbapenam synthetase (CarA) | Erwinia carotovora | adenosine 5'-triphosphate | 0.28 | 0.11 | 2.5 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), adenosine 5'-triphosphate |
| carbapenam synthetase (CarA) | Erwinia carotovora | D,L-2-aminopimelic acid | 0.037 | 9 | 0.0042 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), D,L-2-aminopimelic acid |
| carbapenam synthetase (CarA) | Erwinia carotovora | D-2-aminoadipic acid | 0.2 | 32 | 0.0063 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), D-2-aminoadipic acid |
| carbapenam synthetase (CarA) | Erwinia carotovora | heptanedioic acid | 0.033 | 6 | 0.006 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), heptanedioic acid |
| carbapenam synthetase (CarA) | Erwinia carotovora | hexanedioic acid | 0.082 | 20 | 0.004 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), hexanedioic acid |
| carbapenam synthetase (CarA) | Erwinia carotovora | L-2-aminoadipic acid | 0.19 | 17 | 0.011 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), L-2-aminoadipic acid |
| carbapenam synthetase (CarA) | Erwinia carotovora | L-glutamic acid | 0.028 | 29 | 0.001 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), L-glutamic acid |
| carbapenam synthetase (CarA) | Erwinia carotovora | pentanedioic acid | 0.04 | 27 | 0.0015 | pH 7.8, 22 °C | PMID 12820893 | Open record: carbapenam synthetase (CarA), pentanedioic acid |
| carbapenam synthetase (CPS)variant E380A | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.0067 | 0.49 | 0.014 | pH 8, 25 °C | PMID 19371088 | Open record: carbapenam synthetase (CPS) variant E380A, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant E380D | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.26 | 0.36 | 0.72 | pH 8, 25 °C | PMID 19371088 | Open record: carbapenam synthetase (CPS) variant E380D, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant E380Q | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.02 | 0.075 | 0.27 | pH 8, 25 °C | PMID 19371088 | Open record: carbapenam synthetase (CPS) variant E380Q, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant Y345A | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.011 | 1.7 | 0.0063 | pH 8, 25 °C | PMID 19371088 | Open record: carbapenam synthetase (CPS) variant Y345A, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant Y345A/E380A | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.0051 | 0.4 | 0.012 | pH 8, 25 °C | PMID 19371088 | Open record: carbapenam synthetase (CPS) variant Y345A/E380A, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant Y345F | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.17 | 0.27 | 0.63 | pH 8, 25 °C | PMID 19371088 | Open record: carbapenam synthetase (CPS) variant Y345F, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant Y345F/E380D | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.0059 | 0.49 | 0.012 | pH 8, 25 °C | PMID 19371088 | Open record: carbapenam synthetase (CPS) variant Y345F/E380D, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant Y345F/E380Q | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.011 | 0.9 | 0.011 | pH 8, 25 °C | PMID 19371088 | Open record: carbapenam synthetase (CPS) variant Y345F/E380Q, (2S,5S)-carboxymethylproline |