Values CatLog read from this paper
A conserved tyrosyl-glutamyl catalytic dyad in evolutionarily linked enzymes: carbapenam synthetase and beta-lactam synthetase
PMID 19371088doi:10.1021/bi900432nOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (35 values) from this paper: carbapenam synthetase (CPS), β-lactam synthetase (β-LS) from Pectobacterium carotovorum, Streptomyces clavuligerus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| carbapenam synthetase (CPS)variant E380A | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.0067 | 0.49 | 0.014 | pH 8, 25 °C | Open record: carbapenam synthetase (CPS) variant E380A, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant E380D | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.26 | 0.36 | 0.72 | pH 8, 25 °C | Open record: carbapenam synthetase (CPS) variant E380D, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant E380Q | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.02 | 0.075 | 0.27 | pH 8, 25 °C | Open record: carbapenam synthetase (CPS) variant E380Q, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant Y345A | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.011 | 1.7 | 0.0063 | pH 8, 25 °C | Open record: carbapenam synthetase (CPS) variant Y345A, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant Y345A/E380A | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.0051 | 0.4 | 0.012 | pH 8, 25 °C | Open record: carbapenam synthetase (CPS) variant Y345A/E380A, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant Y345F | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.17 | 0.27 | 0.63 | pH 8, 25 °C | Open record: carbapenam synthetase (CPS) variant Y345F, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant Y345F/E380D | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.0059 | 0.49 | 0.012 | pH 8, 25 °C | Open record: carbapenam synthetase (CPS) variant Y345F/E380D, (2S,5S)-carboxymethylproline |
| carbapenam synthetase (CPS)variant Y345F/E380Q | Pectobacterium carotovorum | (2S,5S)-carboxymethylproline | 0.011 | 0.9 | 0.011 | pH 8, 25 °C | Open record: carbapenam synthetase (CPS) variant Y345F/E380Q, (2S,5S)-carboxymethylproline |
| β-lactam synthetase (β-LS) | Streptomyces clavuligerus | (2S,5S)-carboxymethylproline | 0.026 | 4.9 | 0.0053 calc. | pH 8.3, 25 °C | Open record: β-lactam synthetase (β-LS), (2S,5S)-carboxymethylproline |
| β-lactam synthetase (β-LS)variant E382D | Streptomyces clavuligerus | N2-(2-carboxyethyl)-L-arginine | 0.193 | 2.8 | 0.069 | pH 8.8, 25 °C | Open record: β-lactam synthetase (β-LS) variant E382D, N2-(2-carboxyethyl)-L-arginine |
| β-lactam synthetase (β-LS)variant Y348A | Streptomyces clavuligerus | N2-(2-carboxyethyl)-L-arginine | 0.0082 | 0.19 | 0.043 | pH 8.8, 25 °C | Open record: β-lactam synthetase (β-LS) variant Y348A, N2-(2-carboxyethyl)-L-arginine |
| β-lactam synthetase (β-LS)variant Y348F | Streptomyces clavuligerus | N2-(2-carboxyethyl)-L-arginine | 0.155 | 1.4 | 0.11 | pH 8.8, 25 °C | Open record: β-lactam synthetase (β-LS) variant Y348F, N2-(2-carboxyethyl)-L-arginine |