EC 6.3.5.1: NAD+ synthase (glutamine-hydrolysing)
LigasesOpen in Browse
CatLog read 39 records for EC 6.3.5.1 from 4 papers, for 5 organisms. Names in these papers: NAD+ synthetase (hsNadE), glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM), NAD synthetase NADsyn1 (glutamine-dependent), NAD+ synthetase (tbNadE) and 3 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 38 records for EC 6.3.5.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.5.1 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Different ways to transport ammonia in human and Mycobacterium tuberculosis NAD+ synthetases | Homo sapiens, Mycobacterium tuberculosis | 18 |
| An ancestral glutamine-dependent NAD(+) synthetase revealed by poor kinetic synergism | Thermotoga maritima | 8 |
| Molecular identification of human glutamine- and ammonia-dependent NAD synthetases. Carbon-nitrogen hydrolase domain confers glutamine dependency | Homo sapiens | 7 |
| Kinetics and structural features of dimeric glutamine-dependent bacterial NAD+ synthetases suggest evolutionary adaptation to available metabolites | Azospirillum brasilense, Herbaspirillum seropedicae | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM) | Thermotoga maritima | ammonia | 0.24 | 0.33 | 0.7 | pH 8.3, 37 °C | PMID 19647806 | Open record: glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM), ammonia |
| glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM) | Thermotoga maritima | ATP | 0.15 | 0.095 | 1.6 | pH 8.3, 37 °C | PMID 19647806 | Open record: glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM), ATP |
| glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM) | Thermotoga maritima | glutamineNAD+ formation assay (alcohol dehydrogenase detection); ATP | 0.288 | 0.42 | 0.68 | pH 8.3, 37 °C | PMID 19647806 | Open record: glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM), glutamine |
| glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM) | Thermotoga maritima | glutaminefixed substrates: NaAD+; ATP; cosubstrate NaAD+, ATP | 4.6 | 3 | 1.5 | pH 8.3, 37 °C | PMID 19647806 | Open record: glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM), glutamine |
| glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM) | Thermotoga maritima | glutaminefixed substrates: NaAD+; cosubstrate NaAD+ | 2.4 | 7 | 0.3 | pH 8.3, 37 °C | PMID 19647806 | Open record: glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM), glutamine |
| glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM) | Thermotoga maritima | glutaminefixed substrates: ATP; cosubstrate ATP | 1.9 | 6 | 0.3 | pH 8.3, 37 °C | PMID 19647806 | Open record: glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM), glutamine |
| glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM) | Thermotoga maritima | glutaminefixed substrates: none; basal glutaminase activity | 0.43 | 11 | 0.04 | pH 8.3, 37 °C | PMID 19647806 | Open record: glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM), glutamine |
| glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM) | Thermotoga maritima | nicotinic acid adenine dinucleotide | 0.16 | 0.021 | 7.6 | pH 8.3, 37 °C | PMID 19647806 | Open record: glutamine-dependent NAD+ synthetase (NAD+ synthetaseTM), nicotinic acid adenine dinucleotide |
| NAD synthetase NADsyn1 (glutamine-dependent) | Homo sapiens | adenosine 5'-triphosphate | — | 0.089 | — | pH 7.5 | PMID 12547821 | Open record: NAD synthetase NADsyn1 (glutamine-dependent), adenosine 5'-triphosphate |
| NAD synthetase NADsyn1 (glutamine-dependent) | Homo sapiens | ammonium chloride | — | 13.1 | — | pH 8.8 | PMID 12547821 | Open record: NAD synthetase NADsyn1 (glutamine-dependent), ammonium chloride |
| NAD synthetase NADsyn1 (glutamine-dependent) | Homo sapiens | L-glutamine | — | 1.44 | — | pH 7.5 | PMID 12547821 | Open record: NAD synthetase NADsyn1 (glutamine-dependent), L-glutamine |
| NAD synthetase NADsyn1 (glutamine-dependent) | Homo sapiens | nicotinic acid adenine dinucleotide | — | 0.49 | — | pH 7.5 | PMID 12547821 | Open record: NAD synthetase NADsyn1 (glutamine-dependent), nicotinic acid adenine dinucleotide |
| NAD synthetase NADsyn1 (glutamine-dependent)variant C175S | Homo sapiens | adenosine 5'-triphosphate | — | 0.12 | — | pH 8.8 | PMID 12547821 | Open record: NAD synthetase NADsyn1 (glutamine-dependent) variant C175S, adenosine 5'-triphosphate |
| NAD synthetase NADsyn1 (glutamine-dependent)variant C175S | Homo sapiens | ammonium chloride | — | 23.9 | — | pH 8.8 | PMID 12547821 | Open record: NAD synthetase NADsyn1 (glutamine-dependent) variant C175S, ammonium chloride |
| NAD synthetase NADsyn1 (glutamine-dependent)variant C175S | Homo sapiens | nicotinic acid adenine dinucleotide | — | 0.2 | — | pH 8.8 | PMID 12547821 | Open record: NAD synthetase NADsyn1 (glutamine-dependent) variant C175S, nicotinic acid adenine dinucleotide |
| NAD+ synthetase (hsNadE) | Homo sapiens | adenosine 5'-triphosphateglutamine-dependent reaction; saturating L-glutamine 100 mM | 3.79 | 0.17 | 22 | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), adenosine 5'-triphosphate |
| NAD+ synthetase (hsNadE) | Homo sapiens | adenosine 5'-triphosphateammonia-dependent reaction (NH4Cl as ammonia source) | 18.5 | 0.22 | 84 | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), adenosine 5'-triphosphate |
| NAD+ synthetase (hsNadE) | Homo sapiens | ammonia | 20 | 42 | 0.49 | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), ammonia |
| NAD+ synthetase (hsNadE) | Homo sapiens | L-glutamineglutamine-dependent reaction; saturating NaAD+ 5 mM; Table 1 | 3.72 | 8.3 | 0.45 | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), L-glutamine |
| NAD+ synthetase (hsNadE) | Homo sapiens | L-glutamineglutamine-dependent reaction; saturating NaAD+ 5 mM; Table 1 | 5.6 | 11.6 | 0.48 | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), L-glutamine |
| NAD+ synthetase (hsNadE) | Homo sapiens | L-glutamineglutamine-dependent reaction; no NaAD+ or ATP; Table 1 | 0.18 | 34 | 0.005 | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), L-glutamine |
| NAD+ synthetase (hsNadE) | Homo sapiens | L-glutamineeffectors: NaAD+ (5 mM); Table 2 | 0.316 | — | — | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), L-glutamine |
| NAD+ synthetase (hsNadE) | Homo sapiens | L-glutamineeffectors: NAD+ (4 mM); Table 2 | 0.214 | — | — | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), L-glutamine |
| NAD+ synthetase (hsNadE) | Homo sapiens | L-glutamineeffectors: ATP/Mg2+ (ATP 4 mM); Table 2 | 0.091 | — | — | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), L-glutamine |
| NAD+ synthetase (hsNadE) | Homo sapiens | L-glutamineeffectors: PPi/Mg2+ (PPi 2.5 mM); Table 2 | 0.185 | — | — | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), L-glutamine |
| NAD+ synthetase (hsNadE) | Homo sapiens | L-glutamineeffectors: NAD+ (4 mM); ATP/Mg2+ (ATP 4 mM); Table 2 | 0.113 | — | — | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), L-glutamine |
| NAD+ synthetase (hsNadE) | Homo sapiens | L-glutamineeffectors: NaAD+ (5 mM); AMP/Mg2+ (AMP 5 mM); Table 2 | 0.375 | — | — | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), L-glutamine |
| NAD+ synthetase (hsNadE) | Homo sapiens | L-glutamineeffectors: NaAD+ (5 mM); AMP (5 mM); PPi/Mg2+ (PPi 2.5 mM) | 2.2 | — | — | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), L-glutamine |
| NAD+ synthetase (hsNadE) | Homo sapiens | nicotinic acid adenine dinucleotideglutamine-dependent reaction; saturating L-glutamine 100 mM | 3.2 | 0.26 | 12 | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), nicotinic acid adenine dinucleotide |
| NAD+ synthetase (hsNadE) | Homo sapiens | nicotinic acid adenine dinucleotideammonia-dependent reaction (NH4Cl as ammonia source) | 23.4 | 0.52 | 45 | pH 8, 37 °C | PMID 31911602 | Open record: NAD+ synthetase (hsNadE), nicotinic acid adenine dinucleotide |
| NAD+ synthetase (tbNadE) | Mycobacterium tuberculosis | L-glutamineeffectors: no effectors | 0.0038 | — | — | PMID 31911602 | Open record: NAD+ synthetase (tbNadE), L-glutamine | |
| NAD+ synthetase (tbNadE) | Mycobacterium tuberculosis | L-glutamineeffectors: SFI (5 mM) | 0.0021 | — | — | PMID 31911602 | Open record: NAD+ synthetase (tbNadE), L-glutamine | |
| NAD+ synthetase (tbNadE) | Mycobacterium tuberculosis | L-glutamineeffectors: SFI (5 mM); PPi/Mg2+ (PPi 2.5 mM) | 0.061 | — | — | PMID 31911602 | Open record: NAD+ synthetase (tbNadE), L-glutamine | |
| NadE1^Gln (octameric glutamine-dependent NAD+ synthetase 1, AbNadE1) | Azospirillum brasilense | ammonium | 0.23 | 10.01 | 0.02 | pH 8.5, 30 °C | PMID 29581233 | Open record: NadE1^Gln (octameric glutamine-dependent NAD+ synthetase 1, AbNadE1), ammonium |
| NadE1^Gln (octameric glutamine-dependent NAD+ synthetase 1, AbNadE1) | Azospirillum brasilense | L-glutamine | 0.23 | 0.08 | 2.8 | pH 8.5, 30 °C | PMID 29581233 | Open record: NadE1^Gln (octameric glutamine-dependent NAD+ synthetase 1, AbNadE1), L-glutamine |
| NadE2^Gln (dimeric glutamine-dependent NAD+ synthetase 2, AbNadE2) | Azospirillum brasilense | ammonium | 0.06 | 1.85 | 0.03 | pH 8.5, 30 °C | PMID 29581233 | Open record: NadE2^Gln (dimeric glutamine-dependent NAD+ synthetase 2, AbNadE2), ammonium |
| NadE2^Gln (dimeric glutamine-dependent NAD+ synthetase 2, AbNadE2) | Azospirillum brasilense | L-glutamine | 0.04 | 0.82 | 0.05 | pH 8.5, 30 °C | PMID 29581233 | Open record: NadE2^Gln (dimeric glutamine-dependent NAD+ synthetase 2, AbNadE2), L-glutamine |
| NadE2^Gln (dimeric glutamine-dependent NAD+ synthetase 2, HsNadE2) | Herbaspirillum seropedicae | ammonium | 0.07 | 0.24 | 0.29 | pH 8.5, 30 °C | PMID 29581233 | Open record: NadE2^Gln (dimeric glutamine-dependent NAD+ synthetase 2, HsNadE2), ammonium |
| NadE2^Gln (dimeric glutamine-dependent NAD+ synthetase 2, HsNadE2) | Herbaspirillum seropedicae | L-glutamine | 0.09 | 0.13 | 0.69 | pH 8.5, 30 °C | PMID 29581233 | Open record: NadE2^Gln (dimeric glutamine-dependent NAD+ synthetase 2, HsNadE2), L-glutamine |