CatLogenzyme kinetics catalog

EC 6.3.5.2: GMP synthase (glutamine-hydrolysing)

LigasesOpen in Browse

CatLog read 26 records for EC 6.3.5.2 from 2 papers, for 2 organisms. Names in these papers: GMP synthetase (GMPS), GMP synthase (Gua1).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 48 records for EC 6.3.5.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.5.2 in Browse.

Papers

PaperOrganismsRecords
Conformational changes involving ammonia tunnel formation and allosteric control in GMP synthetaseEscherichia coli23
GMP Synthase Is Required for Virulence Factor Production and Infection by Cryptococcus neoformansCryptococcus neoformans3

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
GMP synthase (Gua1)Cryptococcus neoformansadenosine 5'-triphosphate—0.0775—pH 7.5, 40 °CPMID 28062578Open
GMP synthase (Gua1)Cryptococcus neoformansL-glutamine—1.13—pH 7.5, 40 °CPMID 28062578Open
GMP synthase (Gua1)Cryptococcus neoformansxanthosine 5'-monophosphate0.410.06596.2 calc.pH 7.5, 40 °CPMID 28062578Open
GMP synthetase (GMPS)Escherichia coliglutamine13.71.68PMID 24434004Open
GMP synthetase (GMPS)Escherichia coliglutamine29.31.7516.7pH 8.5, 37 °CPMID 24434004Open
GMP synthetase (GMPS)Escherichia colixanthosine 5'-monophosphateglutamine-dependent; cosubstrate glutamine, ATP230.053430PMID 24434004Open
GMP synthetase (GMPS)Escherichia colixanthosine 5'-monophosphateammonia-dependent (200 mM NH4Cl replacing glutamine)13.30.043310PMID 24434004Open
GMP synthetase (GMPS)variant E383AEscherichia coliglutamine3.23.21PMID 24434004Open
GMP synthetase (GMPS)variant E383AEscherichia coliglutaminebasal glutaminase (no XMP; water replaced XMP)0.0021401.5 × 10⁻⁵pH 8.5, 37 °CPMID 24434004Open
GMP synthetase (GMPS)variant E383AEscherichia coliglutamine200 µM XMP; cosubstrate XMP, ATP5.52.72pH 8.5, 37 °CPMID 24434004Open
GMP synthetase (GMPS)variant E383AEscherichia colixanthosine 5'-monophosphateglutamine-dependent; cosubstrate glutamine, ATP3.20.022140PMID 24434004Open
GMP synthetase (GMPS)variant E383AEscherichia colixanthosine 5'-monophosphateammonia-dependent (200 mM NH4Cl replacing glutamine)70.056120PMID 24434004Open
GMP synthetase (GMPS)variant H186AEscherichia coliglutamine16800.21PMID 24434004Open
GMP synthetase (GMPS)variant H186AEscherichia coliglutamine5.2600.08pH 8.5, 37 °CPMID 24434004Open
GMP synthetase (GMPS)variant H186AEscherichia colixanthosine 5'-monophosphateglutamine-dependent; cosubstrate glutamine, ATP140.045310PMID 24434004Open
GMP synthetase (GMPS)variant H186AEscherichia colixanthosine 5'-monophosphateammonia-dependent (200 mM NH4Cl replacing glutamine)15.60.05310PMID 24434004Open
GMP synthetase (GMPS)variant H186A/E383AEscherichia coliglutamine4.8990.049PMID 24434004Open
GMP synthetase (GMPS)variant H186A/E383AEscherichia coliglutaminebasal glutaminase (no XMP; water replaced XMP)0.0051304.1 × 10⁻⁵pH 8.5, 37 °CPMID 24434004Open
GMP synthetase (GMPS)variant H186A/E383AEscherichia coliglutamine200 µM XMP; cosubstrate XMP, ATP5.3700.07pH 8.5, 37 °CPMID 24434004Open
GMP synthetase (GMPS)variant H186A/E383AEscherichia colixanthosine 5'-monophosphateglutamine-dependent; cosubstrate glutamine, ATP0.850.007110PMID 24434004Open
GMP synthetase (GMPS)variant H186A/E383AEscherichia colixanthosine 5'-monophosphateammonia-dependent (200 mM NH4Cl replacing glutamine)90.074120PMID 24434004Open
GMP synthetase (GMPS)variant H186E/E383HEscherichia coliglutamine4.7670.07PMID 24434004Open
GMP synthetase (GMPS)variant H186E/E383HEscherichia coliglutaminebasal glutaminase (no XMP; water replaced XMP)0.0081705 × 10⁻⁵pH 8.5, 37 °CPMID 24434004Open
GMP synthetase (GMPS)variant H186E/E383HEscherichia coliglutamine200 µM XMP; cosubstrate XMP, ATP7460.15pH 8.5, 37 °CPMID 24434004Open
GMP synthetase (GMPS)variant H186E/E383HEscherichia colixanthosine 5'-monophosphateglutamine-dependent; cosubstrate glutamine, ATP0.810.008100PMID 24434004Open
GMP synthetase (GMPS)variant H186E/E383HEscherichia colixanthosine 5'-monophosphateammonia-dependent (200 mM NH4Cl replacing glutamine)6.10.05120PMID 24434004Open