CatLogenzyme kinetics catalog

Values CatLog read from this paper

Conformational changes involving ammonia tunnel formation and allosteric control in GMP synthetase

PMID 24434004doi:10.1016/j.abb.2014.01.004Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 23 records (69 values) from this paper: GMP synthetase (GMPS) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.3.5.2

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
GMP synthetase (GMPS)Escherichia coliglutamine13.71.68Open
GMP synthetase (GMPS)Escherichia coliglutamine29.31.7516.7pH 8.5, 37 °COpen
GMP synthetase (GMPS)Escherichia colixanthosine 5'-monophosphateglutamine-dependent; cosubstrate glutamine, ATP230.053430Open
GMP synthetase (GMPS)Escherichia colixanthosine 5'-monophosphateammonia-dependent (200 mM NH4Cl replacing glutamine)13.30.043310Open
GMP synthetase (GMPS)variant E383AEscherichia coliglutamine3.23.21Open
GMP synthetase (GMPS)variant E383AEscherichia coliglutaminebasal glutaminase (no XMP; water replaced XMP)0.0021401.5 × 10⁻⁵pH 8.5, 37 °COpen
GMP synthetase (GMPS)variant E383AEscherichia coliglutamine200 µM XMP; cosubstrate XMP, ATP5.52.72pH 8.5, 37 °COpen
GMP synthetase (GMPS)variant E383AEscherichia colixanthosine 5'-monophosphateglutamine-dependent; cosubstrate glutamine, ATP3.20.022140Open
GMP synthetase (GMPS)variant E383AEscherichia colixanthosine 5'-monophosphateammonia-dependent (200 mM NH4Cl replacing glutamine)70.056120Open
GMP synthetase (GMPS)variant H186AEscherichia coliglutamine16800.21Open
GMP synthetase (GMPS)variant H186AEscherichia coliglutamine5.2600.08pH 8.5, 37 °COpen
GMP synthetase (GMPS)variant H186AEscherichia colixanthosine 5'-monophosphateglutamine-dependent; cosubstrate glutamine, ATP140.045310Open
GMP synthetase (GMPS)variant H186AEscherichia colixanthosine 5'-monophosphateammonia-dependent (200 mM NH4Cl replacing glutamine)15.60.05310Open
GMP synthetase (GMPS)variant H186A/E383AEscherichia coliglutamine4.8990.049Open
GMP synthetase (GMPS)variant H186A/E383AEscherichia coliglutaminebasal glutaminase (no XMP; water replaced XMP)0.0051304.1 × 10⁻⁵pH 8.5, 37 °COpen
GMP synthetase (GMPS)variant H186A/E383AEscherichia coliglutamine200 µM XMP; cosubstrate XMP, ATP5.3700.07pH 8.5, 37 °COpen
GMP synthetase (GMPS)variant H186A/E383AEscherichia colixanthosine 5'-monophosphateglutamine-dependent; cosubstrate glutamine, ATP0.850.007110Open
GMP synthetase (GMPS)variant H186A/E383AEscherichia colixanthosine 5'-monophosphateammonia-dependent (200 mM NH4Cl replacing glutamine)90.074120Open
GMP synthetase (GMPS)variant H186E/E383HEscherichia coliglutamine4.7670.07Open
GMP synthetase (GMPS)variant H186E/E383HEscherichia coliglutaminebasal glutaminase (no XMP; water replaced XMP)0.0081705 × 10⁻⁵pH 8.5, 37 °COpen
GMP synthetase (GMPS)variant H186E/E383HEscherichia coliglutamine200 µM XMP; cosubstrate XMP, ATP7460.15pH 8.5, 37 °COpen
GMP synthetase (GMPS)variant H186E/E383HEscherichia colixanthosine 5'-monophosphateglutamine-dependent; cosubstrate glutamine, ATP0.810.008100Open
GMP synthetase (GMPS)variant H186E/E383HEscherichia colixanthosine 5'-monophosphateammonia-dependent (200 mM NH4Cl replacing glutamine)6.10.05120Open