EC 6.3.5.4: asparagine synthase (glutamine-hydrolysing)
LigasesOpen in Browse
CatLog read 38 records for EC 6.3.5.4 from 2 papers, for 3 organisms. Names in these papers: asparagine synthetase B (AS-B), asparagine synthetase ZmAsnS2, asparagine synthetase ZmAsnS1, asparagine synthetase ZmAsnS3 and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 81 records for EC 6.3.5.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.5.4 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| A kinetic comparison of asparagine synthetase isozymes from higher plants | Glycine max, Zea mays | 22 |
| A conserved glutamate controls the commitment to acyl-adenylate formation in asparagine synthetase | Escherichia coli | 16 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| asparagine synthetase B (AS-B) | Escherichia coli | adenosine 5'-triphosphateglutamine-dependent synthetase activity | 0.9 | 0.1 | 9 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B), adenosine 5'-triphosphate |
| asparagine synthetase B (AS-B) | Escherichia coli | adenosine 5'-triphosphateammonia-dependent synthetase activity | 0.96 | 0.11 | 8.7 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B), adenosine 5'-triphosphate |
| asparagine synthetase B (AS-B) | Escherichia coli | L-aspartic acidglutamine-dependent synthetase activity | 0.67 | 0.58 | 1.2 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B), L-aspartic acid |
| asparagine synthetase B (AS-B) | Escherichia coli | L-aspartic acidammonia-dependent synthetase activity | 0.75 | 1.2 | 0.63 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B), L-aspartic acid |
| asparagine synthetase B (AS-B) | Escherichia coli | L-glutamineglutaminase activity (L-glutamine hydrolysis); no ATP | 6.2 | 5 | 1.19 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B), L-glutamine |
| asparagine synthetase B (AS-B) | Escherichia coli | L-glutamine | 6.6 | 1.7 | 3.88 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B), L-glutamine |
| asparagine synthetase B (AS-B)variant E348A | Escherichia coli | L-glutamineglutaminase activity (L-glutamine hydrolysis); no ATP | 6.37 | 5.8 | 1.1 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B) variant E348A, L-glutamine |
| asparagine synthetase B (AS-B)variant E348A | Escherichia coli | L-glutamine | 5.8 | 3.5 | 1.66 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B) variant E348A, L-glutamine |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | adenosine 5'-triphosphateglutamine-dependent synthetase activity | 0.51 | 0.013 | 39.2 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B) variant E348D, adenosine 5'-triphosphate |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | adenosine 5'-triphosphateammonia-dependent synthetase activity | 0.43 | 0.03 | 14.3 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B) variant E348D, adenosine 5'-triphosphate |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | L-aspartic acidglutamine-dependent synthetase activity | 0.45 | 0.13 | 3.45 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B) variant E348D, L-aspartic acid |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | L-aspartic acidammonia-dependent synthetase activity | 0.3 | 0.23 | 1.3 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B) variant E348D, L-aspartic acid |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | L-glutamineglutaminase activity (L-glutamine hydrolysis); no ATP | 4.1 | 2.7 | 1.51 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B) variant E348D, L-glutamine |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | L-glutamine | 10.02 | 1.1 | 9.1 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B) variant E348D, L-glutamine |
| asparagine synthetase B (AS-B)variant E348Q | Escherichia coli | L-glutamineglutaminase activity (L-glutamine hydrolysis); no ATP | 4 | 9 | 0.45 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B) variant E348Q, L-glutamine |
| asparagine synthetase B (AS-B)variant E348Q | Escherichia coli | L-glutamine | 4.45 | 3.9 | 1.14 | pH 8, 37 °C | PMID 20853825 | Open record: asparagine synthetase B (AS-B) variant E348Q, L-glutamine |
| asparagine synthetase GmAsnS | Glycine max | adenosine 5'-triphosphate | — | 0.106 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase GmAsnS, adenosine 5'-triphosphate |
| asparagine synthetase GmAsnS | Glycine max | aspartate | — | 0.85 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase GmAsnS, aspartate |
| asparagine synthetase GmAsnS | Glycine max | glutamine | — | 0.254 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase GmAsnS, glutamine |
| asparagine synthetase ZmAsnS1 | Zea mays | adenosine 5'-triphosphate | — | 0.11 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS1, adenosine 5'-triphosphate |
| asparagine synthetase ZmAsnS1 | Zea mays | ammonium | — | 9.9 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS1, ammonium |
| asparagine synthetase ZmAsnS1 | Zea mays | aspartate | — | 0.98 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS1, aspartate |
| asparagine synthetase ZmAsnS1 | Zea mays | glutamine | — | 0.543 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS1, glutamine |
| asparagine synthetase ZmAsnS2 | Zea mays | adenosine 5'-triphosphatenon-tagged | — | 0.125 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS2, adenosine 5'-triphosphate |
| asparagine synthetase ZmAsnS2 | Zea mays | adenosine 5'-triphosphateC-terminal His-tagged | — | 0.128 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS2, adenosine 5'-triphosphate |
| asparagine synthetase ZmAsnS2 | Zea mays | ammonium | — | 0.75 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS2, ammonium |
| asparagine synthetase ZmAsnS2 | Zea mays | aspartatenon-tagged | — | 0.91 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS2, aspartate |
| asparagine synthetase ZmAsnS2 | Zea mays | aspartateC-terminal His-tagged | — | 0.98 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS2, aspartate |
| asparagine synthetase ZmAsnS2 | Zea mays | glutaminenon-tagged | — | 0.11 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS2, glutamine |
| asparagine synthetase ZmAsnS2 | Zea mays | glutamineC-terminal His-tagged | — | 0.09 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS2, glutamine |
| asparagine synthetase ZmAsnS3 | Zea mays | adenosine 5'-triphosphate | — | 0.097 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS3, adenosine 5'-triphosphate |
| asparagine synthetase ZmAsnS3 | Zea mays | ammonium | — | 8.4 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS3, ammonium |
| asparagine synthetase ZmAsnS3 | Zea mays | aspartate | — | 1.2 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS3, aspartate |
| asparagine synthetase ZmAsnS3 | Zea mays | glutamine | — | 0.423 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS3, glutamine |
| asparagine synthetase ZmAsnS4 | Zea mays | adenosine 5'-triphosphate | — | 0.128 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS4, adenosine 5'-triphosphate |
| asparagine synthetase ZmAsnS4 | Zea mays | ammonium | — | 9 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS4, ammonium |
| asparagine synthetase ZmAsnS4 | Zea mays | aspartate | — | 0.93 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS4, aspartate |
| asparagine synthetase ZmAsnS4 | Zea mays | glutamine | — | 0.233 | — | pH 7.6 | PMID 21276727 | Open record: asparagine synthetase ZmAsnS4, glutamine |