Values CatLog read from this paper
A conserved glutamate controls the commitment to acyl-adenylate formation in asparagine synthetase
PMID 20853825doi:10.1021/bi1010688Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (48 values) from this paper: asparagine synthetase B (AS-B) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| asparagine synthetase B (AS-B) | Escherichia coli | adenosine 5'-triphosphateglutamine-dependent synthetase activity | 0.9 | 0.1 | 9 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B), adenosine 5'-triphosphate |
| asparagine synthetase B (AS-B) | Escherichia coli | adenosine 5'-triphosphateammonia-dependent synthetase activity | 0.96 | 0.11 | 8.7 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B), adenosine 5'-triphosphate |
| asparagine synthetase B (AS-B) | Escherichia coli | L-aspartic acidglutamine-dependent synthetase activity | 0.67 | 0.58 | 1.2 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B), L-aspartic acid |
| asparagine synthetase B (AS-B) | Escherichia coli | L-aspartic acidammonia-dependent synthetase activity | 0.75 | 1.2 | 0.63 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B), L-aspartic acid |
| asparagine synthetase B (AS-B) | Escherichia coli | L-glutamineglutaminase activity (L-glutamine hydrolysis); no ATP | 6.2 | 5 | 1.19 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B), L-glutamine |
| asparagine synthetase B (AS-B) | Escherichia coli | L-glutamine | 6.6 | 1.7 | 3.88 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B), L-glutamine |
| asparagine synthetase B (AS-B)variant E348A | Escherichia coli | L-glutamineglutaminase activity (L-glutamine hydrolysis); no ATP | 6.37 | 5.8 | 1.1 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B) variant E348A, L-glutamine |
| asparagine synthetase B (AS-B)variant E348A | Escherichia coli | L-glutamine | 5.8 | 3.5 | 1.66 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B) variant E348A, L-glutamine |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | adenosine 5'-triphosphateglutamine-dependent synthetase activity | 0.51 | 0.013 | 39.2 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B) variant E348D, adenosine 5'-triphosphate |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | adenosine 5'-triphosphateammonia-dependent synthetase activity | 0.43 | 0.03 | 14.3 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B) variant E348D, adenosine 5'-triphosphate |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | L-aspartic acidglutamine-dependent synthetase activity | 0.45 | 0.13 | 3.45 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B) variant E348D, L-aspartic acid |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | L-aspartic acidammonia-dependent synthetase activity | 0.3 | 0.23 | 1.3 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B) variant E348D, L-aspartic acid |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | L-glutamineglutaminase activity (L-glutamine hydrolysis); no ATP | 4.1 | 2.7 | 1.51 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B) variant E348D, L-glutamine |
| asparagine synthetase B (AS-B)variant E348D | Escherichia coli | L-glutamine | 10.02 | 1.1 | 9.1 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B) variant E348D, L-glutamine |
| asparagine synthetase B (AS-B)variant E348Q | Escherichia coli | L-glutamineglutaminase activity (L-glutamine hydrolysis); no ATP | 4 | 9 | 0.45 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B) variant E348Q, L-glutamine |
| asparagine synthetase B (AS-B)variant E348Q | Escherichia coli | L-glutamine | 4.45 | 3.9 | 1.14 | pH 8, 37 °C | Open record: asparagine synthetase B (AS-B) variant E348Q, L-glutamine |