Values CatLog read from this paper

(S)-Mandelate dehydrogenase from Pseudomonas putida: mechanistic studies with alternate substrates and pH and kinetic isotope effects

PMID 10231535doi:10.1021/bi990024mOpen in Browse

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CatLog read 20 records (25 values) from this paper: (S)-mandelate dehydrogenase from Pseudomonas putida.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.99.31

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-deuterio-2-hydroxy-2-phenylacetate	105	—	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-deuterio-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-2-(1H-indol-3-yl)acetate	—	0.4	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-2-(1H-indol-3-yl)acetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-2-phenylacetate	260	0.33	790 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-3-(1H-indol-3-yl)propanoate	—	0.9	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-3-(1H-indol-3-yl)propanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-3-methylbutanoate	—	9.5	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-3-methylbutanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-3-phenylpropanoate	—	2	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-3-phenylpropanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-3-phenylpropanoate	1.1	—	—	20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-3-phenylpropanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-3-phenylpropanoate	1.8	—	—	20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-3-phenylpropanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-4-methylpentanoate	—	4.3	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-4-methylpentanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxybut-3-enoate	—	15.4	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxybut-3-enoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxybut-3-ynoate	—	22	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxybut-3-ynoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxybutanoate	—	32	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxybutanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxyhexanoate	—	4.9	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxyhexanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxyoctanoate	—	0.75	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxyoctanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxypentanoate	—	15.3	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxypentanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(S)-2-deuterio-2-hydroxy-2-phenylacetate	118	0.167	707 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (S)-2-deuterio-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(S)-2-hydroxy-2-phenylacetatein H2O	290	0.16	1.8 × 10³ calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(S)-2-hydroxy-2-phenylacetatein D2O (pD 7.5)	240	0.11	2.2 × 10³ calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(S)-2-hydroxy-2-phenylacetate	301	—	545	20 °C	Open record: (S)-mandelate dehydrogenase, (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	hexacyanoferrate(III)	—	9	—	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, hexacyanoferrate(III)