EC 1.1.99.31: (S)-mandelate dehydrogenase

OxidoreductasesOpen in Browse

CatLog read 86 records for EC 1.1.99.31 from 6 papers, for 2 organisms. Names in these papers: (S)-mandelate dehydrogenase, L-(+)-mandelate dehydrogenase (L-MDH), L-mandelate dehydrogenase (flavocytochrome b2).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 39 records for EC 1.1.99.31 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.1.99.31 in Browse.

Papers

Papers with records for EC 1.1.99.31
Paper	Organisms	Records
Role of glycine 81 in (S)-mandelate dehydrogenase from Pseudomonas putida in substrate specificity and oxidase activity	Pseudomonas putida	26
(S)-Mandelate dehydrogenase from Pseudomonas putida: mechanistic studies with alternate substrates and pH and kinetic isotope effects	Pseudomonas putida	20
Substrate analogues as probes of the catalytic mechanism of L-mandelate dehydrogenase from Rhodotorula graminis	Rhodotorula graminis	11
Arginine 165/arginine 277 pair in (S)-mandelate dehydrogenase from Pseudomonas putida: role in catalysis and substrate binding	Pseudomonas putida	10
Esters of mandelic acid as substrates for (S)-mandelate dehydrogenase from Pseudomonas putida: implications for the reaction mechanism	Pseudomonas putida	10
L-Mandelate dehydrogenase from Rhodotorula graminis: cloning, sequencing and kinetic characterization of the recombinant enzyme and its independently expressed flavin domain	Rhodotorula graminis	9

Records

Records for EC 1.1.99.31 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-deuterio-2-hydroxy-2-phenylacetate	105	—	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-deuterio-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-2-(1H-indol-3-yl)acetate	—	0.4	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-2-(1H-indol-3-yl)acetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-2-phenylacetate	260	0.33	790 calc.	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-3-(1H-indol-3-yl)propanoate	—	0.9	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-3-(1H-indol-3-yl)propanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-3-methylbutanoate	—	9.5	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-3-methylbutanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-3-phenylpropanoate	—	2	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-3-phenylpropanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-3-phenylpropanoate	1.1	—	—	20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-3-phenylpropanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-3-phenylpropanoate	1.8	—	—	20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-3-phenylpropanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxy-4-methylpentanoate	—	4.3	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxy-4-methylpentanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxybut-3-enoate	—	15.4	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxybut-3-enoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxybut-3-ynoate	—	22	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxybut-3-ynoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxybutanoate	—	32	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxybutanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxyhexanoate	—	4.9	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxyhexanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxyoctanoate	—	0.75	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxyoctanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(RS)-2-hydroxypentanoate	—	15.3	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (RS)-2-hydroxypentanoate
(S)-mandelate dehydrogenase	Pseudomonas putida	(S)-2-deuterio-2-hydroxy-2-phenylacetate	118	0.167	707 calc.	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (S)-2-deuterio-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(S)-2-hydroxy-2-phenylacetatein H2O	290	0.16	1.8 × 10³ calc.	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(S)-2-hydroxy-2-phenylacetatein D2O (pD 7.5)	240	0.11	2.2 × 10³ calc.	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(S)-2-hydroxy-2-phenylacetate	301	—	545	20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	(S)-mandelate	360	0.12	3.0 × 10³ calc.	pH 7.5, 20 °C	PMID 12369819	Open record: (S)-mandelate dehydrogenase, (S)-mandelate
(S)-mandelate dehydrogenase	Pseudomonas putida	4-chloromandelate	334	0.27	1237	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase, 4-chloromandelate
(S)-mandelate dehydrogenase	Pseudomonas putida	dioxygen	1.2	3.2	0.38 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase, dioxygen
(S)-mandelate dehydrogenase	Pseudomonas putida	ethyl (S)-2-hydroxy-2-phenylacetate	204	2.9	70 calc.	pH 7.5, 20 °C	PMID 14967029	Open record: (S)-mandelate dehydrogenase, ethyl (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	ethyl (S)-2-hydroxy-2-phenylacetate	78	2.4	32 calc.	pH 7.5, 4 °C	PMID 14967029	Open record: (S)-mandelate dehydrogenase, ethyl (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	hexacyanoferrate(III)	—	9	—	pH 7.5, 20 °C	PMID 10231535	Open record: (S)-mandelate dehydrogenase, hexacyanoferrate(III)
(S)-mandelate dehydrogenase	Pseudomonas putida	mandelate	350	0.24	1458	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase, mandelate
(S)-mandelate dehydrogenase	Pseudomonas putida	methyl (S)-2-hydroxy-2-phenylacetate	151	11.3	13.4 calc.	pH 7.5, 20 °C	PMID 14967029	Open record: (S)-mandelate dehydrogenase, methyl (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenase	Pseudomonas putida	methyl (S)-2-hydroxy-2-phenylacetate	55.9	7.7	7.3 calc.	pH 7.5, 4 °C	PMID 14967029	Open record: (S)-mandelate dehydrogenase, methyl (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenaseMDH-GOX2 (chimera)	Pseudomonas putida	(S)-mandelate	205	0.09	2.3 × 10³ calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase MDH-GOX2 (chimera), (S)-mandelate
(S)-mandelate dehydrogenaseMDH-GOX2 (chimera)	Pseudomonas putida	dioxygen	0.03	0.68	0.044 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase MDH-GOX2 (chimera), dioxygen
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	(S)-mandelate	19.2	0.15	128	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, (S)-mandelate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxy-3-butynoate	14.8	4.3	3.4	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxy-3-butynoate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxybutyrate	0.37	4.4	0.08	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxybutyrate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxyhexanoate	0.19	1.4	0.14	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxyhexanoate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxyisocaproate	0.48	1.6	0.3	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxyisocaproate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxyoctanoate	0.26	0.14	1.9	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxyoctanoate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxyvalerate	0.27	6.4	0.04	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxyvalerate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	3-indolelactate	0.27	0.17	1.6	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, 3-indolelactate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	3-phenyllactate	0.15	0.37	0.4	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, 3-phenyllactate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	4-chloromandelate	9.3	0.43	21.6	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, 4-chloromandelate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	dioxygen	3.4	1.1	3.1 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, dioxygen
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	indoleglycolate	1.2	0.63	1.9	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, indoleglycolate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	mandelate	15.2	0.24	63.3	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A, mandelate
(S)-mandelate dehydrogenasevariant G81A (G81A/MDH-GOX2 chimera)	Pseudomonas putida	(S)-mandelate	2.3	0.04	58 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A (G81A/MDH-GOX2 chimera), (S)-mandelate
(S)-mandelate dehydrogenasevariant G81A (G81A/MDH-GOX2 chimera)	Pseudomonas putida	dioxygen	0.22	0.49	0.45 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81A (G81A/MDH-GOX2 chimera), dioxygen
(S)-mandelate dehydrogenasevariant G81D	Pseudomonas putida	(S)-mandelate	0.11	2	0.055 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81D, (S)-mandelate
(S)-mandelate dehydrogenasevariant G81D	Pseudomonas putida	dioxygen	0.05	0.22	0.23 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81D, dioxygen
(S)-mandelate dehydrogenasevariant G81S	Pseudomonas putida	(S)-mandelate	2.8	2.3	1.2 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81S, (S)-mandelate
(S)-mandelate dehydrogenasevariant G81S	Pseudomonas putida	dioxygen	0.61	1.4	0.44 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81S, dioxygen
(S)-mandelate dehydrogenasevariant G81V	Pseudomonas putida	(S)-mandelate	0.05	0.17	0.29 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81V, (S)-mandelate
(S)-mandelate dehydrogenasevariant G81V	Pseudomonas putida	dioxygen	0.07	0.04	1.8 calc.	pH 7.5, 20 °C	PMID 15311930	Open record: (S)-mandelate dehydrogenase variant G81V, dioxygen
(S)-mandelate dehydrogenasevariant R165E	Pseudomonas putida	(S)-mandelate	4.4	12	0.37 calc.	pH 7.5, 20 °C	PMID 12369819	Open record: (S)-mandelate dehydrogenase variant R165E, (S)-mandelate
(S)-mandelate dehydrogenasevariant R165G	Pseudomonas putida	(S)-mandelate	13.2	5.8	2.3 calc.	pH 7.5, 20 °C	PMID 12369819	Open record: (S)-mandelate dehydrogenase variant R165G, (S)-mandelate
(S)-mandelate dehydrogenasevariant R165G/R277K	Pseudomonas putida	(S)-mandelate	0.04	31.4	0.0013 calc.	pH 7.5, 20 °C	PMID 12369819	Open record: (S)-mandelate dehydrogenase variant R165G/R277K, (S)-mandelate
(S)-mandelate dehydrogenasevariant R165K	Pseudomonas putida	(S)-mandelate	120	1.5	80 calc.	pH 7.5, 20 °C	PMID 12369819	Open record: (S)-mandelate dehydrogenase variant R165K, (S)-mandelate
(S)-mandelate dehydrogenasevariant R165K/R277G	Pseudomonas putida	(S)-mandelate	0.53	17.6	0.030 calc.	pH 7.5, 20 °C	PMID 12369819	Open record: (S)-mandelate dehydrogenase variant R165K/R277G, (S)-mandelate
(S)-mandelate dehydrogenasevariant R165K/R277K	Pseudomonas putida	(S)-mandelate	1.1	15.2	0.072 calc.	pH 7.5, 20 °C	PMID 12369819	Open record: (S)-mandelate dehydrogenase variant R165K/R277K, (S)-mandelate
(S)-mandelate dehydrogenasevariant R165M	Pseudomonas putida	(S)-mandelate	18.4	4.4	4.2 calc.	pH 7.5, 20 °C	PMID 12369819	Open record: (S)-mandelate dehydrogenase variant R165M, (S)-mandelate
(S)-mandelate dehydrogenasevariant R277G	Pseudomonas putida	(S)-mandelate	0.23	17	0.014 calc.	pH 7.5, 20 °C	PMID 12369819	Open record: (S)-mandelate dehydrogenase variant R277G, (S)-mandelate
(S)-mandelate dehydrogenasevariant R277G	Pseudomonas putida	ethyl (S)-2-hydroxy-2-phenylacetate	0.3	3.7	0.081 calc.	pH 7.5, 20 °C	PMID 14967029	Open record: (S)-mandelate dehydrogenase variant R277G, ethyl (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenasevariant R277G	Pseudomonas putida	ethyl (S)-2-hydroxy-2-phenylacetate	10	7	1.4 calc.	pH 7.5	PMID 14967029	Open record: (S)-mandelate dehydrogenase variant R277G, ethyl (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenasevariant R277G	Pseudomonas putida	methyl (S)-2-hydroxy-2-phenylacetate	2.5	7.4	0.34 calc.	pH 7.5, 20 °C	PMID 14967029	Open record: (S)-mandelate dehydrogenase variant R277G, methyl (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenasevariant R277G	Pseudomonas putida	methyl (S)-2-hydroxy-2-phenylacetate	8	5.5	1.5 calc.	pH 7.5	PMID 14967029	Open record: (S)-mandelate dehydrogenase variant R277G, methyl (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenasevariant R277K	Pseudomonas putida	(S)-mandelate	66	5.6	12 calc.	pH 7.5, 20 °C	PMID 12369819	Open record: (S)-mandelate dehydrogenase variant R277K, (S)-mandelate
(S)-mandelate dehydrogenasevariant R277K	Pseudomonas putida	ethyl (S)-2-hydroxy-2-phenylacetate	2.6	2.7	0.96 calc.	pH 7.5, 20 °C	PMID 14967029	Open record: (S)-mandelate dehydrogenase variant R277K, ethyl (S)-2-hydroxy-2-phenylacetate
(S)-mandelate dehydrogenasevariant R277K	Pseudomonas putida	methyl (S)-2-hydroxy-2-phenylacetate	2.3	10.3	0.22 calc.	pH 7.5, 20 °C	PMID 14967029	Open record: (S)-mandelate dehydrogenase variant R277K, methyl (S)-2-hydroxy-2-phenylacetate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	3-hydroxy-D,L-mandelate	108	0.15	740	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), 3-hydroxy-D,L-mandelate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	3-methoxy-D,L-mandelate	106	0.12	870	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), 3-methoxy-D,L-mandelate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	4-bromo-D,L-mandelate	108	0.26	410	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), 4-bromo-D,L-mandelate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	4-chloro-D,L-mandelate	116	0.38	300	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), 4-chloro-D,L-mandelate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	4-fluoro-D,L-mandelate	98	0.16	600	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), 4-fluoro-D,L-mandelate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	4-hydroxy-D,L-mandelate	146	0.08	1.9 × 10³	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), 4-hydroxy-D,L-mandelate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	4-methoxy-D,L-mandelate	68	0.12	570	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), 4-methoxy-D,L-mandelate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	4-methyl-D,L-mandelate	80	0.17	480	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), 4-methyl-D,L-mandelate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	D,L-mandelate	94	0.35	260	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), D,L-mandelate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	DL-[2-2H]Mandelate	93	0.74	130	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), DL-[2-2H]Mandelate
L-(+)-mandelate dehydrogenase (L-MDH)	Rhodotorula graminis	L-(+)-mandelate	114	0.24	470	pH 7.5, 25 °C	PMID 8110205	Open record: L-(+)-mandelate dehydrogenase (L-MDH), L-(+)-mandelate
L-mandelate dehydrogenase (flavocytochrome b2)	Rhodotorula graminis	DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate)1 mM ferricyanide (saturating); cosubstrate ferricyanide	88	0.53	170 calc.	pH 7.5, 25 °C	PMID 9639569	Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate)
L-mandelate dehydrogenase (flavocytochrome b2)	Rhodotorula graminis	DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate)6 mM ferricyanide (saturating); cosubstrate ferricyanide	99	0.79	130 calc.	pH 7.5, 25 °C	PMID 9639569	Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate)
L-mandelate dehydrogenase (flavocytochrome b2)	Rhodotorula graminis	DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate)35 µM cytochrome c (horse type VI, saturating)	92	1.33	69 calc.	pH 7.5, 25 °C	PMID 9639569	Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate)
L-mandelate dehydrogenase (flavocytochrome b2)	Rhodotorula graminis	DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition)1 mM ferricyanide (saturating); cosubstrate ferricyanide	300	0.47	640 calc.	pH 7.5, 25 °C	PMID 9639569	Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition)
L-mandelate dehydrogenase (flavocytochrome b2)	Rhodotorula graminis	DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition)6 mM ferricyanide (saturating); cosubstrate ferricyanide	316	0.74	430 calc.	pH 7.5, 25 °C	PMID 9639569	Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition)
L-mandelate dehydrogenase (flavocytochrome b2)	Rhodotorula graminis	DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition)35 µM cytochrome c (horse type VI, saturating)	155	0.78	200 calc.	pH 7.5, 25 °C	PMID 9639569	Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition)
L-mandelate dehydrogenase (flavocytochrome b2)	Rhodotorula graminis	L-2-hydroxy-2-phenylacetate1 mM ferricyanide (saturating); cosubstrate ferricyanide	550	0.35	1.6 × 10³ calc.	pH 7.5, 25 °C	PMID 9639569	Open record: L-mandelate dehydrogenase (flavocytochrome b2), L-2-hydroxy-2-phenylacetate
L-mandelate dehydrogenase (flavocytochrome b2)	Rhodotorula graminis	L-2-hydroxy-2-phenylacetate6 mM ferricyanide (saturating); cosubstrate ferricyanide	500	0.33	1.5 × 10³ calc.	pH 7.5, 25 °C	PMID 9639569	Open record: L-mandelate dehydrogenase (flavocytochrome b2), L-2-hydroxy-2-phenylacetate
L-mandelate dehydrogenase (flavocytochrome b2)	Rhodotorula graminis	L-2-hydroxy-2-phenylacetate35 µM cytochrome c (horse type VI, saturating)	225	0.35	640 calc.	pH 7.5, 25 °C	PMID 9639569	Open record: L-mandelate dehydrogenase (flavocytochrome b2), L-2-hydroxy-2-phenylacetate