Values CatLog read from this paper
Arginine 165/arginine 277 pair in (S)-mandelate dehydrogenase from Pseudomonas putida: role in catalysis and substrate binding
PMID 12369819doi:10.1021/bi026258eOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (20 values) from this paper: (S)-mandelate dehydrogenase from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| (S)-mandelate dehydrogenase | Pseudomonas putida | (S)-mandelate | 360 | 0.12 | 3.0 × 10³ calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase, (S)-mandelate |
| (S)-mandelate dehydrogenasevariant R165E | Pseudomonas putida | (S)-mandelate | 4.4 | 12 | 0.37 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R165E, (S)-mandelate |
| (S)-mandelate dehydrogenasevariant R165G | Pseudomonas putida | (S)-mandelate | 13.2 | 5.8 | 2.3 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R165G, (S)-mandelate |
| (S)-mandelate dehydrogenasevariant R165G/R277K | Pseudomonas putida | (S)-mandelate | 0.04 | 31.4 | 0.0013 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R165G/R277K, (S)-mandelate |
| (S)-mandelate dehydrogenasevariant R165K | Pseudomonas putida | (S)-mandelate | 120 | 1.5 | 80 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R165K, (S)-mandelate |
| (S)-mandelate dehydrogenasevariant R165K/R277G | Pseudomonas putida | (S)-mandelate | 0.53 | 17.6 | 0.030 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R165K/R277G, (S)-mandelate |
| (S)-mandelate dehydrogenasevariant R165K/R277K | Pseudomonas putida | (S)-mandelate | 1.1 | 15.2 | 0.072 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R165K/R277K, (S)-mandelate |
| (S)-mandelate dehydrogenasevariant R165M | Pseudomonas putida | (S)-mandelate | 18.4 | 4.4 | 4.2 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R165M, (S)-mandelate |
| (S)-mandelate dehydrogenasevariant R277G | Pseudomonas putida | (S)-mandelate | 0.23 | 17 | 0.014 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R277G, (S)-mandelate |
| (S)-mandelate dehydrogenasevariant R277K | Pseudomonas putida | (S)-mandelate | 66 | 5.6 | 12 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R277K, (S)-mandelate |