Values CatLog read from this paper

Role of glycine 81 in (S)-mandelate dehydrogenase from Pseudomonas putida in substrate specificity and oxidase activity

PMID 15311930doi:10.1021/bi049005pOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 26 records (66 values) from this paper: (S)-mandelate dehydrogenase from Pseudomonas putida.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.99.31

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
(S)-mandelate dehydrogenase	Pseudomonas putida	4-chloromandelate	334	0.27	1237	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, 4-chloromandelate
(S)-mandelate dehydrogenase	Pseudomonas putida	dioxygen	1.2	3.2	0.38 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, dioxygen
(S)-mandelate dehydrogenase	Pseudomonas putida	mandelate	350	0.24	1458	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase, mandelate
(S)-mandelate dehydrogenaseMDH-GOX2 (chimera)	Pseudomonas putida	(S)-mandelate	205	0.09	2.3 × 10³ calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase MDH-GOX2 (chimera), (S)-mandelate
(S)-mandelate dehydrogenaseMDH-GOX2 (chimera)	Pseudomonas putida	dioxygen	0.03	0.68	0.044 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase MDH-GOX2 (chimera), dioxygen
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	(S)-mandelate	19.2	0.15	128	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, (S)-mandelate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxy-3-butynoate	14.8	4.3	3.4	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxy-3-butynoate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxybutyrate	0.37	4.4	0.08	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxybutyrate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxyhexanoate	0.19	1.4	0.14	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxyhexanoate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxyisocaproate	0.48	1.6	0.3	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxyisocaproate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxyoctanoate	0.26	0.14	1.9	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxyoctanoate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	2-hydroxyvalerate	0.27	6.4	0.04	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, 2-hydroxyvalerate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	3-indolelactate	0.27	0.17	1.6	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, 3-indolelactate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	3-phenyllactate	0.15	0.37	0.4	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, 3-phenyllactate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	4-chloromandelate	9.3	0.43	21.6	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, 4-chloromandelate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	dioxygen	3.4	1.1	3.1 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, dioxygen
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	indoleglycolate	1.2	0.63	1.9	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, indoleglycolate
(S)-mandelate dehydrogenasevariant G81A	Pseudomonas putida	mandelate	15.2	0.24	63.3	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A, mandelate
(S)-mandelate dehydrogenasevariant G81A (G81A/MDH-GOX2 chimera)	Pseudomonas putida	(S)-mandelate	2.3	0.04	58 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A (G81A/MDH-GOX2 chimera), (S)-mandelate
(S)-mandelate dehydrogenasevariant G81A (G81A/MDH-GOX2 chimera)	Pseudomonas putida	dioxygen	0.22	0.49	0.45 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81A (G81A/MDH-GOX2 chimera), dioxygen
(S)-mandelate dehydrogenasevariant G81D	Pseudomonas putida	(S)-mandelate	0.11	2	0.055 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81D, (S)-mandelate
(S)-mandelate dehydrogenasevariant G81D	Pseudomonas putida	dioxygen	0.05	0.22	0.23 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81D, dioxygen
(S)-mandelate dehydrogenasevariant G81S	Pseudomonas putida	(S)-mandelate	2.8	2.3	1.2 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81S, (S)-mandelate
(S)-mandelate dehydrogenasevariant G81S	Pseudomonas putida	dioxygen	0.61	1.4	0.44 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81S, dioxygen
(S)-mandelate dehydrogenasevariant G81V	Pseudomonas putida	(S)-mandelate	0.05	0.17	0.29 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81V, (S)-mandelate
(S)-mandelate dehydrogenasevariant G81V	Pseudomonas putida	dioxygen	0.07	0.04	1.8 calc.	pH 7.5, 20 °C	Open record: (S)-mandelate dehydrogenase variant G81V, dioxygen