Values CatLog read from this paper
Esters of mandelic acid as substrates for (S)-mandelate dehydrogenase from Pseudomonas putida: implications for the reaction mechanism
PMID 14967029doi:10.1021/bi036021yOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (20 values) from this paper: (S)-mandelate dehydrogenase from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| (S)-mandelate dehydrogenase | Pseudomonas putida | ethyl (S)-2-hydroxy-2-phenylacetate | 204 | 2.9 | 70 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase, ethyl (S)-2-hydroxy-2-phenylacetate |
| (S)-mandelate dehydrogenase | Pseudomonas putida | ethyl (S)-2-hydroxy-2-phenylacetate | 78 | 2.4 | 32 calc. | pH 7.5, 4 °C | Open record: (S)-mandelate dehydrogenase, ethyl (S)-2-hydroxy-2-phenylacetate |
| (S)-mandelate dehydrogenase | Pseudomonas putida | methyl (S)-2-hydroxy-2-phenylacetate | 151 | 11.3 | 13.4 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase, methyl (S)-2-hydroxy-2-phenylacetate |
| (S)-mandelate dehydrogenase | Pseudomonas putida | methyl (S)-2-hydroxy-2-phenylacetate | 55.9 | 7.7 | 7.3 calc. | pH 7.5, 4 °C | Open record: (S)-mandelate dehydrogenase, methyl (S)-2-hydroxy-2-phenylacetate |
| (S)-mandelate dehydrogenasevariant R277G | Pseudomonas putida | ethyl (S)-2-hydroxy-2-phenylacetate | 0.3 | 3.7 | 0.081 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R277G, ethyl (S)-2-hydroxy-2-phenylacetate |
| (S)-mandelate dehydrogenasevariant R277G | Pseudomonas putida | ethyl (S)-2-hydroxy-2-phenylacetate | 10 | 7 | 1.4 calc. | pH 7.5 | Open record: (S)-mandelate dehydrogenase variant R277G, ethyl (S)-2-hydroxy-2-phenylacetate |
| (S)-mandelate dehydrogenasevariant R277G | Pseudomonas putida | methyl (S)-2-hydroxy-2-phenylacetate | 2.5 | 7.4 | 0.34 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R277G, methyl (S)-2-hydroxy-2-phenylacetate |
| (S)-mandelate dehydrogenasevariant R277G | Pseudomonas putida | methyl (S)-2-hydroxy-2-phenylacetate | 8 | 5.5 | 1.5 calc. | pH 7.5 | Open record: (S)-mandelate dehydrogenase variant R277G, methyl (S)-2-hydroxy-2-phenylacetate |
| (S)-mandelate dehydrogenasevariant R277K | Pseudomonas putida | ethyl (S)-2-hydroxy-2-phenylacetate | 2.6 | 2.7 | 0.96 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R277K, ethyl (S)-2-hydroxy-2-phenylacetate |
| (S)-mandelate dehydrogenasevariant R277K | Pseudomonas putida | methyl (S)-2-hydroxy-2-phenylacetate | 2.3 | 10.3 | 0.22 calc. | pH 7.5, 20 °C | Open record: (S)-mandelate dehydrogenase variant R277K, methyl (S)-2-hydroxy-2-phenylacetate |