Values CatLog read from this paper
L-Mandelate dehydrogenase from Rhodotorula graminis: cloning, sequencing and kinetic characterization of the recombinant enzyme and its independently expressed flavin domain
PMID 9639569doi:10.1042/bj3330107Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (18 values) from this paper: L-mandelate dehydrogenase (flavocytochrome b2) from Rhodotorula graminis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| L-mandelate dehydrogenase (flavocytochrome b2) | Rhodotorula graminis | DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate)1 mM ferricyanide (saturating); cosubstrate ferricyanide | 88 | 0.53 | 170 calc. | pH 7.5, 25 °C | Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate) |
| L-mandelate dehydrogenase (flavocytochrome b2) | Rhodotorula graminis | DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate)6 mM ferricyanide (saturating); cosubstrate ferricyanide | 99 | 0.79 | 130 calc. | pH 7.5, 25 °C | Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate) |
| L-mandelate dehydrogenase (flavocytochrome b2) | Rhodotorula graminis | DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate)35 µM cytochrome c (horse type VI, saturating) | 92 | 1.33 | 69 calc. | pH 7.5, 25 °C | Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-[2-2H]mandelic acid (racemic 2-deuterio-2-hydroxy-2-phenylacetate) |
| L-mandelate dehydrogenase (flavocytochrome b2) | Rhodotorula graminis | DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition)1 mM ferricyanide (saturating); cosubstrate ferricyanide | 300 | 0.47 | 640 calc. | pH 7.5, 25 °C | Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition) |
| L-mandelate dehydrogenase (flavocytochrome b2) | Rhodotorula graminis | DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition)6 mM ferricyanide (saturating); cosubstrate ferricyanide | 316 | 0.74 | 430 calc. | pH 7.5, 25 °C | Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition) |
| L-mandelate dehydrogenase (flavocytochrome b2) | Rhodotorula graminis | DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition)35 µM cytochrome c (horse type VI, saturating) | 155 | 0.78 | 200 calc. | pH 7.5, 25 °C | Open record: L-mandelate dehydrogenase (flavocytochrome b2), DL-mandelic acid (racemic 2-hydroxy-2-phenylacetate, normal isotopic composition) |
| L-mandelate dehydrogenase (flavocytochrome b2) | Rhodotorula graminis | L-2-hydroxy-2-phenylacetate1 mM ferricyanide (saturating); cosubstrate ferricyanide | 550 | 0.35 | 1.6 × 10³ calc. | pH 7.5, 25 °C | Open record: L-mandelate dehydrogenase (flavocytochrome b2), L-2-hydroxy-2-phenylacetate |
| L-mandelate dehydrogenase (flavocytochrome b2) | Rhodotorula graminis | L-2-hydroxy-2-phenylacetate6 mM ferricyanide (saturating); cosubstrate ferricyanide | 500 | 0.33 | 1.5 × 10³ calc. | pH 7.5, 25 °C | Open record: L-mandelate dehydrogenase (flavocytochrome b2), L-2-hydroxy-2-phenylacetate |
| L-mandelate dehydrogenase (flavocytochrome b2) | Rhodotorula graminis | L-2-hydroxy-2-phenylacetate35 µM cytochrome c (horse type VI, saturating) | 225 | 0.35 | 640 calc. | pH 7.5, 25 °C | Open record: L-mandelate dehydrogenase (flavocytochrome b2), L-2-hydroxy-2-phenylacetate |