Values CatLog read from this paper
Kinetic specificities of BPN' and Carlsberg subtilisins. Mapping the aromatic binding site
PMID 102640doi:10.1093/oxfordjournals.jbchem.a132157Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 49 records (147 values) from this paper: subtilisin Carlsberg, subtilisin BPN' from Bacillus licheniformis, Bacillus amyloliquefaciens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| subtilisin BPN' | Bacillus amyloliquefaciens | N,O-diacetyl-3-nitro-L-tyrosine methyl ester | 6.9 | 0.63 | 11 | pH 7.8, 25 °C | Open record: subtilisin BPN', N,O-diacetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N,O-diacetyl-3-nitro-L-tyrosine methyl ester | 0.9 | 0.39 | 2.3 | pH 6.5, 25 °C | Open record: subtilisin BPN', N,O-diacetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N,O-diacetyl-L-tyrosine methyl ester | 22.7 | 1.31 | 17 | pH 7.8, 25 °C | Open record: subtilisin BPN', N,O-diacetyl-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N,O-diacetyl-L-tyrosine methyl ester | 2.5 | 0.8 | 3 | pH 6.5, 25 °C | Open record: subtilisin BPN', N,O-diacetyl-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-1-formyl-L-tryptophan methyl ester | 46.3 | 10.6 | 4.4 | pH 7.2, 25 °C | Open record: subtilisin BPN', N-acetyl-1-formyl-L-tryptophan methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-1-formyl-L-tryptophan methyl ester | 15 | 4.2 | 3.6 | pH 6.5, 25 °C | Open record: subtilisin BPN', N-acetyl-1-formyl-L-tryptophan methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-2-hydroxy-L-phenylalanine methyl ester | 1.61 | 0.53 | 3 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-2-hydroxy-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-2-hydroxy-L-tryptophan methyl ester | 1.3 | 3.2 | 0.41 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-2-hydroxy-L-tryptophan methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester | 80.2 | 5.6 | 14 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester | 8.4 | 4.1 | 2 | pH 6.5, 25 °C | Open record: subtilisin BPN', N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3-hydroxy-L-phenylalanine methyl ester | 59.1 | 5.03 | 12 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-3-hydroxy-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3-hydroxy-L-phenylalanine methyl ester | 16 | 12.5 | 1.3 | pH 6.5, 25 °C | Open record: subtilisin BPN', N-acetyl-3-hydroxy-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3-nitro-L-tyrosine methyl ester | 38 | 7.9 | 4.8 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3-nitro-L-tyrosine methyl ester | 31.4 | 5.1 | 6.2 | pH 6.5, 25 °C | Open record: subtilisin BPN', N-acetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-4-acetamido-L-phenylalanine methyl ester | 2.9 | 1.1 | 2.6 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-4-acetamido-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-4-nitro-L-phenylalanine methyl ester | 421 | 16.7 | 25 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-4-nitro-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-4-nitro-L-phenylalanine methyl ester | 25.1 | 7.8 | 3.2 | pH 6.5, 25 °C | Open record: subtilisin BPN', N-acetyl-4-nitro-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-phenylalanine methyl ester | 76.6 | 9.3 | 8.2 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-phenylalanine methyl ester | 6.9 | 6.2 | 1.1 | pH 6.5, 25 °C | Open record: subtilisin BPN', N-acetyl-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-tryptophan methyl ester | 37.9 | 4 | 9.5 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-L-tryptophan methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-tryptophan methyl ester | 2.4 | 2 | 1.2 | pH 6.5, 25 °C | Open record: subtilisin BPN', N-acetyl-L-tryptophan methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-tyrosine methyl ester | 418 | 18.6 | 23 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-tyrosine methyl ester | 36.4 | 17.2 | 2.1 | pH 6.5, 25 °C | Open record: subtilisin BPN', N-acetyl-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-O-[4-[(E)-phenyldiazenyl]benzoyl]-L-tyrosine methyl ester | 3.8 | 0.65 | 5.9 | pH 7.8, 25 °C | Open record: subtilisin BPN', N-acetyl-O-[4-[(E)-phenyldiazenyl]benzoyl]-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N,O-diacetyl-3-nitro-L-tyrosine methyl ester | 163 | 6.1 | 27 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N,O-diacetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N,O-diacetyl-3-nitro-L-tyrosine methyl ester | 66 | 22.7 | 2.9 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N,O-diacetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N,O-diacetyl-L-tyrosine methyl ester | 721 | 9.5 | 76 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N,O-diacetyl-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N,O-diacetyl-L-tyrosine methyl ester | 59 | 6.2 | 9.5 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N,O-diacetyl-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-1-formyl-L-tryptophan methyl ester | 265 | 9.3 | 28 | pH 7.2, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-1-formyl-L-tryptophan methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-1-formyl-L-tryptophan methyl ester | 38.1 | 5.1 | 7.5 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-1-formyl-L-tryptophan methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-2-hydroxy-L-phenylalanine methyl ester | 5.3 | 3 | 1.8 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-2-hydroxy-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-2-hydroxy-L-tryptophan methyl ester | 3.7 | 1.4 | 2.6 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-2-hydroxy-L-tryptophan methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester | 370 | 14 | 26 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester | 27 | 8.7 | 3.1 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3-hydroxy-L-phenylalanine methyl ester | 187 | 3 | 62 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-3-hydroxy-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3-hydroxy-L-phenylalanine methyl ester | 19.9 | 4 | 5 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-3-hydroxy-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3-nitro-L-tyrosine methyl ester | 349 | 31.2 | 11 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3-nitro-L-tyrosine methyl ester | 22 | 2.2 | 10 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-4-acetamido-L-phenylalanine methyl ester | 221 | 13.4 | 16 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-4-acetamido-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-4-acetamido-L-phenylalanine methyl ester | 18.3 | 10.5 | 1.7 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-4-acetamido-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-4-nitro-L-phenylalanine methyl ester | 5.52 × 10³ | 32.6 | 169 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-4-nitro-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-4-nitro-L-phenylalanine methyl ester | 805 | 37.3 | 22 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-4-nitro-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-phenylalanine methyl ester | 353 | 7.9 | 45 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-phenylalanine methyl ester | 18.8 | 3.4 | 5.5 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-tryptophan methyl ester | 444 | 7.2 | 62 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-L-tryptophan methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-tryptophan methyl ester | 54 | 13 | 4.2 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-L-tryptophan methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-tyrosine methyl ester | 862 | 16.4 | 53 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-tyrosine methyl ester | 88 | 15.9 | 5.5 | pH 6.5, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-O-[4-[(E)-phenyldiazenyl]benzoyl]-L-tyrosine methyl ester | 27.1 | 1.3 | 21 | pH 7.8, 25 °C | Open record: subtilisin Carlsberg, N-acetyl-O-[4-[(E)-phenyldiazenyl]benzoyl]-L-tyrosine methyl ester |