EC 3.4.21.14: microbial serine proteases
HydrolasesOpen in Browse
CatLog read 87 records for EC 3.4.21.14 from 3 papers, for 3 organisms. Names in these papers: subtilisin Bacillus lentus (SBL), subtilisin Carlsberg, subtilisin BPN'.
EC 3.4.21.14 is no longer current; BRENDA: “transferred to EC 3.4.21.62, EC 3.4.21.63, EC 3.4.21.64, EC 3.4.21.65, EC 3.4.21.66, EC 3.4.21.67”.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Kinetic specificities of BPN' and Carlsberg subtilisins. Mapping the aromatic binding site | Bacillus amyloliquefaciens, Bacillus licheniformis | 49 |
| Toward tailoring the specificity of the S1 pocket of subtilisin B. lentus: chemical modification of mutant enzymes as a strategy for removing specificity limitations | Bacillus lentus | 21 |
| Site-directed mutagenesis combined with chemical modification as a strategy for altering the specificity of the S1 and S1' pockets of subtilisin Bacillus lentus | Bacillus lentus | 17 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| subtilisin Bacillus lentus (SBL) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide | 17.7 | 2 | 8.8 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL), succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 0.16 | 7.2 | 0.022 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-glutamyl-para-nitroanilide | 1.75 | 4.4 | 0.4 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL), succinyl-alanyl-alanyl-prolyl-glutamyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 153 | 0.73 | 209 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant M222C-S-b | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 7.2 | 0.63 | 11 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant M222C-S-b, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant M222C-S-c | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 0.49 | 0.69 | 0.71 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant M222C-S-c, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant M222C-S-d | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 6.9 | 0.67 | 10.3 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant M222C-S-d, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 43 | 0.65 | 66 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S156C, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-a | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 30 | 0.79 | 38 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-a, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-b | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 23.4 | 0.68 | 34 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-b, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-c | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 24.2 | 0.6 | 40 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-c, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-d | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 21.8 | 0.54 | 40 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-d, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-e | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 31.6 | 0.78 | 40 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-e, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-f | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 39 | 0.86 | 45 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-f, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 14.2 | 0.51 | 28 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S166C, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2(CH2)2CH2COO- (S166C-S-h) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 0.35 | 8.2 | 0.041 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2(CH2)2CH2COO- (S166C-S-h), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2(CH2)8CH3 (S166C-S-e) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide | 6.8 | 1.9 | 3.6 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2(CH2)8CH3 (S166C-S-e), succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2(CH2)8CH3 (S166C-S-e) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 82 | 1.09 | 75 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2(CH2)8CH3 (S166C-S-e), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H11 (S166C-S-f) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide | 28.2 | 1.9 | 14.8 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H11 (S166C-S-f), succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H11 (S166C-S-f) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 4.8 | 0.7 | 6.9 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H11 (S166C-S-f), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H4-3,5-(COO-)2 (S166C-S-d) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 0.68 | 5.5 | 0.12 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H4-3,5-(COO-)2 (S166C-S-d), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H4-3,5-(COO-)2 (S166C-S-d) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 47 | 1.6 | 29 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H4-3,5-(COO-)2 (S166C-S-d), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H5 (S166C-S-c) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide | 6.8 | 0.8 | 9 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H5 (S166C-S-c), succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H5 (S166C-S-c) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 23.1 | 1.17 | 20 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H5 (S166C-S-c), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2C(CH3)(COO-)2 (S166C-S-i) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 0.43 | 5.3 | 0.08 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2C(CH3)(COO-)2 (S166C-S-i), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2C(COO-)3 (S166C-S-j) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 1.06 | 5.2 | 0.2 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2C(COO-)3 (S166C-S-j), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2NH3+ (S166C-S-a) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-glutamyl-para-nitroanilide | 14.5 | 1.9 | 7.6 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2NH3+ (S166C-S-a), succinyl-alanyl-alanyl-prolyl-glutamyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2NH3+ (S166C-S-a) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 50 | 0.68 | 74 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2NH3+ (S166C-S-a), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2SO3- (S166C-S-b) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 0.17 | 3.4 | 0.05 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2SO3- (S166C-S-b), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2SO3- (S166C-S-b) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 25 | 1.34 | 19 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2SO3- (S166C-S-b), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-a | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 17.4 | 0.39 | 45 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-a, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-b | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 11.8 | 0.92 | 12.8 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-b, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-c | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 31 | 0.72 | 47 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-c, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-d | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 6.9 | 0.74 | 9 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-d, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-e | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 3.8 | 0.7 | 5.4 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-e, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-f | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 16.3 | 0.6 | 27 | pH 7.5, 25 °C | PMID 9558332 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-f, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-steroidyl (S166C-S-g) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide | 9.65 | 1.74 | 5.54 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-steroidyl (S166C-S-g), succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-steroidyl (S166C-S-g) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 29 | 0.74 | 41 | pH 8.6, 25 °C | PMID 10529215 | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-steroidyl (S166C-S-g), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin BPN' | Bacillus amyloliquefaciens | N,O-diacetyl-3-nitro-L-tyrosine methyl ester | 6.9 | 0.63 | 11 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N,O-diacetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N,O-diacetyl-3-nitro-L-tyrosine methyl ester | 0.9 | 0.39 | 2.3 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin BPN', N,O-diacetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N,O-diacetyl-L-tyrosine methyl ester | 22.7 | 1.31 | 17 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N,O-diacetyl-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N,O-diacetyl-L-tyrosine methyl ester | 2.5 | 0.8 | 3 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin BPN', N,O-diacetyl-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-1-formyl-L-tryptophan methyl ester | 46.3 | 10.6 | 4.4 | pH 7.2, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-1-formyl-L-tryptophan methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-1-formyl-L-tryptophan methyl ester | 15 | 4.2 | 3.6 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-1-formyl-L-tryptophan methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-2-hydroxy-L-phenylalanine methyl ester | 1.61 | 0.53 | 3 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-2-hydroxy-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-2-hydroxy-L-tryptophan methyl ester | 1.3 | 3.2 | 0.41 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-2-hydroxy-L-tryptophan methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester | 80.2 | 5.6 | 14 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester | 8.4 | 4.1 | 2 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3-hydroxy-L-phenylalanine methyl ester | 59.1 | 5.03 | 12 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-3-hydroxy-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3-hydroxy-L-phenylalanine methyl ester | 16 | 12.5 | 1.3 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-3-hydroxy-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3-nitro-L-tyrosine methyl ester | 38 | 7.9 | 4.8 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-3-nitro-L-tyrosine methyl ester | 31.4 | 5.1 | 6.2 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-4-acetamido-L-phenylalanine methyl ester | 2.9 | 1.1 | 2.6 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-4-acetamido-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-4-nitro-L-phenylalanine methyl ester | 421 | 16.7 | 25 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-4-nitro-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-4-nitro-L-phenylalanine methyl ester | 25.1 | 7.8 | 3.2 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-4-nitro-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-phenylalanine methyl ester | 76.6 | 9.3 | 8.2 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-phenylalanine methyl ester | 6.9 | 6.2 | 1.1 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-L-phenylalanine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-tryptophan methyl ester | 37.9 | 4 | 9.5 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-L-tryptophan methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-tryptophan methyl ester | 2.4 | 2 | 1.2 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-L-tryptophan methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-tyrosine methyl ester | 418 | 18.6 | 23 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-L-tyrosine methyl ester | 36.4 | 17.2 | 2.1 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-L-tyrosine methyl ester |
| subtilisin BPN' | Bacillus amyloliquefaciens | N-acetyl-O-[4-[(E)-phenyldiazenyl]benzoyl]-L-tyrosine methyl ester | 3.8 | 0.65 | 5.9 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin BPN', N-acetyl-O-[4-[(E)-phenyldiazenyl]benzoyl]-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N,O-diacetyl-3-nitro-L-tyrosine methyl ester | 163 | 6.1 | 27 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N,O-diacetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N,O-diacetyl-3-nitro-L-tyrosine methyl ester | 66 | 22.7 | 2.9 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N,O-diacetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N,O-diacetyl-L-tyrosine methyl ester | 721 | 9.5 | 76 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N,O-diacetyl-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N,O-diacetyl-L-tyrosine methyl ester | 59 | 6.2 | 9.5 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N,O-diacetyl-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-1-formyl-L-tryptophan methyl ester | 265 | 9.3 | 28 | pH 7.2, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-1-formyl-L-tryptophan methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-1-formyl-L-tryptophan methyl ester | 38.1 | 5.1 | 7.5 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-1-formyl-L-tryptophan methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-2-hydroxy-L-phenylalanine methyl ester | 5.3 | 3 | 1.8 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-2-hydroxy-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-2-hydroxy-L-tryptophan methyl ester | 3.7 | 1.4 | 2.6 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-2-hydroxy-L-tryptophan methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester | 370 | 14 | 26 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester | 27 | 8.7 | 3.1 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-3,4-dihydroxy-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3-hydroxy-L-phenylalanine methyl ester | 187 | 3 | 62 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-3-hydroxy-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3-hydroxy-L-phenylalanine methyl ester | 19.9 | 4 | 5 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-3-hydroxy-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3-nitro-L-tyrosine methyl ester | 349 | 31.2 | 11 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-3-nitro-L-tyrosine methyl ester | 22 | 2.2 | 10 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-3-nitro-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-4-acetamido-L-phenylalanine methyl ester | 221 | 13.4 | 16 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-4-acetamido-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-4-acetamido-L-phenylalanine methyl ester | 18.3 | 10.5 | 1.7 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-4-acetamido-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-4-nitro-L-phenylalanine methyl ester | 5.52 × 10³ | 32.6 | 169 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-4-nitro-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-4-nitro-L-phenylalanine methyl ester | 805 | 37.3 | 22 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-4-nitro-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-phenylalanine methyl ester | 353 | 7.9 | 45 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-phenylalanine methyl ester | 18.8 | 3.4 | 5.5 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-L-phenylalanine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-tryptophan methyl ester | 444 | 7.2 | 62 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-L-tryptophan methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-tryptophan methyl ester | 54 | 13 | 4.2 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-L-tryptophan methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-tyrosine methyl ester | 862 | 16.4 | 53 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-L-tyrosine methyl ester | 88 | 15.9 | 5.5 | pH 6.5, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-L-tyrosine methyl ester |
| subtilisin Carlsberg | Bacillus licheniformis | N-acetyl-O-[4-[(E)-phenyldiazenyl]benzoyl]-L-tyrosine methyl ester | 27.1 | 1.3 | 21 | pH 7.8, 25 °C | PMID 102640 | Open record: subtilisin Carlsberg, N-acetyl-O-[4-[(E)-phenyldiazenyl]benzoyl]-L-tyrosine methyl ester |