CatLogenzyme kinetics catalog

EC 3.4.21.62: Subtilisin

HydrolasesOpen in Browse

CatLog read 114 records for EC 3.4.21.62 from 11 papers, for 6 organisms. Names in these papers: subtilisin E, subtilisin Carlsberg, Ak.1 protease, savinase and 5 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 75 records for EC 3.4.21.62 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.62 in Browse.

Papers

PaperOrganismsRecords
Redirecting catalysis from proteolysis to perhydrolysis in subtilisin Carlsberg—25
Purification and characterization of Ak.1 protease, a thermostable subtilisin with a disulphide bond in the substrate-binding cleftBacillus sp.21
Random mutagenesis into the conserved Gly154 of subtilisin E: isolation and characterization of the revertant enzymesBacillus subtilis16
Engineering a substrate-specific cold-adapted subtilisinBacillus lentus10
Restriction of substrate specificity of subtilisin E by introduction of a side chain into a conserved glycine residueBacillus subtilis10
Kinetic and molecular properties of Bacillus subtilis IBTC-3 subtilisinBacillus subtilis7
Gly or Ala substitutions for Pro(210)Thr(211)Asn(212) at the β8-β9 turn of subtilisin Carlsberg increase the catalytic rate and decrease thermostabilityBacillus licheniformis7
Functional analysis of propeptide as an intramolecular chaperone for in vivo folding of subtilisin nattokinaseBacillus subtilis var. natto6
Probing the importance of hydrogen bonds in the active site of the subtilisin nattokinase by site-directed mutagenesis and molecular dynamics simulationBacillus subtilis var. natto5
Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidationBacillus amyloliquefaciens5
Comprehensive biochemical, molecular and structural characterization of subtilisin with fibrinolytic potential in bioprocessingBacillus subtilis2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
Ak.1 proteaseBacillus sp.N-benzyloxycarbonyl-Gly p-nitrophenyl estercontrol: no DTT (disulphide bond intact)1.9 × 10³8.3230pH 7.5, 20 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-benzyloxycarbonyl-Gly p-nitrophenyl ester+10 mM DTT (disulphide bond reduced)2.5 × 10³8.3300pH 7.5, 20 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Ala-p-nitroanilide2220.28790pH 7.5, 5 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Ala-p-nitroanilide2.2 × 10⁴0.633.5 × 10⁴pH 7.5, 40 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Ala-p-nitroanilide9.7 × 10⁴2.254.3 × 10⁴pH 7.5, 75 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Ala-p-nitroanilidecontrol: no DTT (disulphide bond intact)1.2 × 10³0.323.8 × 10³pH 7.5, 20 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Ala-p-nitroanilide+10 mM DTT (disulphide bond reduced)7500.581.3 × 10³pH 7.5, 20 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-p-nitroanilide3.7 × 10³17220pH 7.5, 75 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-p-nitroanilidecontrol: no DTT (disulphide bond intact)93424pH 7.5, 20 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-p-nitroanilide+10 mM DTT (disulphide bond reduced)936.6714pH 7.5, 20 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide2220.33670pH 7.5, 5 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide8.8 × 10³0.332.7 × 10⁴pH 7.5, 40 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide1.3 × 10⁵0.921.4 × 10⁵pH 7.5, 75 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide3890.68570pH 7.5, 5 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide8.8 × 10⁴1.018.7 × 10⁴pH 7.5, 40 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide8.2 × 10⁶3.372.4 × 10⁶pH 7.5, 75 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide1480.072.1 × 10³pH 7.5, 5 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide1.3 × 10⁵0.34.3 × 10⁵pH 7.5, 40 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide1.1 × 10⁷11.1 × 10⁷ calc.pH 7.5, 75 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilidecontrol: no DTT (disulphide bond intact)1 × 10⁴0.156.7 × 10⁴pH 7.5, 20 °CPMID 10926860Open
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide+10 mM DTT (disulphide bond reduced)2.9 × 10³0.446.6 × 10³pH 7.5, 20 °CPMID 10926860Open
nattokinase (subtilisin NK)Bacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide52.10.196265.3pH 8.6, 25 °CPMID 16411898Open
nattokinase (subtilisin NK)variant D60ABacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide3.70.5277pH 8.6, 25 °CPMID 16411898Open
nattokinase (subtilisin NK)variant S33ABacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide16.50.35846.1pH 8.6, 25 °CPMID 16411898Open
nattokinase (subtilisin NK)variant S62ABacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide8.20.38621.2pH 8.6, 25 °CPMID 16411898Open
nattokinase (subtilisin NK)variant T220ABacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide6.50.43215pH 8.6, 25 °CPMID 16411898Open
savinaseBacillus lentusN-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide471.99224pH 9, 24 °CPMID 15047911Open
savinaseBacillus lentusN-succinyl-Ala-Ala-Val-Ala-p-nitroanilide120.039294pH 9, 24 °CPMID 15047911Open
savinaseBacillus lentusN-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide1851.156160pH 9, 24 °CPMID 15047911Open
savinaseBacillus lentusN-succinyl-Phe-Ala-Ala-Phe-p-nitroanilide900.223418pH 9, 24 °CPMID 15047911Open
savinaseBacillus lentusN-succinyl-Tyr-Val-Ala-Asp-p-nitroanilide100.61816pH 9, 24 °CPMID 15047911Open
savinasevariant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid)Bacillus lentusN-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide310.98330pH 9, 24 °CPMID 15047911Open
savinasevariant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid)Bacillus lentusN-succinyl-Ala-Ala-Val-Ala-p-nitroanilide100.053184pH 9, 24 °CPMID 15047911Open
savinasevariant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid)Bacillus lentusN-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide4031.926209pH 9, 24 °CPMID 15047911Open
savinasevariant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid)Bacillus lentusN-succinyl-Phe-Ala-Ala-Phe-p-nitroanilide1890.241767pH 9, 24 °CPMID 15047911Open
savinasevariant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid)Bacillus lentusN-succinyl-Tyr-Val-Ala-Asp-p-nitroanilide40.7466pH 9, 24 °CPMID 15047911Open
subtilisinBacillus amyloliquefaciensN-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide500.14360pH 8.6, 25 °CPMID 3922976Open
subtilisinvariant M222ABacillus amyloliquefaciensN-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide400.7350pH 8.6, 25 °CPMID 3922976Open
subtilisinvariant M222CBacillus amyloliquefaciensN-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide840.48200pH 8.6, 25 °CPMID 3922976Open
subtilisinvariant M222LBacillus amyloliquefaciensN-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide50.2620pH 8.6, 25 °CPMID 3922976Open
subtilisinvariant M222SBacillus amyloliquefaciensN-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide270.6340pH 8.6, 25 °CPMID 3922976Open
subtilisinBacillus subtiliscasein38.570.95240.5PMID 40117024Open
subtilisinBacillus subtilisN-succinyl-Phe-Ala-Ala-Phe-p-nitroanilide290.73139.7PMID 40117024Open
subtilisin Carlsberg—Hydrogen peroxide1.1410.0267pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsberg—Methyl-butyrate2.1117.70.0183pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsberg—Methyl-pentanoate1.235.20.0333pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsberg—Methyl-propionate1.1478.20.0023pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsberg—N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide172.60.59292.5pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165I/L216W (M2)—Hydrogen peroxide3.120.90.1467pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165I/L216W (M2)—Methyl-butyrate10.193.10.1083pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165I/L216W (M2)—Methyl-pentanoate6.329.20.217pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165I/L216W (M2)—Methyl-propionate3.4255.20.0133pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165I/L216W (M2)—N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide1.70.543.1pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165L/L216W (M1)—Hydrogen peroxide2.918.80.1533pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165L/L216W (M1)—Methyl-butyrate11.364.70.1733pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165L/L216W (M1)—Methyl-pentanoate6.433.30.1917pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165L/L216W (M1)—Methyl-propionate3265.10.0115pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165L/L216W (M1)—N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide15.10.4236pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165Y/L216W (M3)—Hydrogen peroxide3.518.70.1867pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165Y/L216W (M3)—Methyl-butyrate8.888.80.0983pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165Y/L216W (M3)—Methyl-pentanoate326.30.115pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165Y/L216W (M3)—Methyl-propionate4349.40.0113pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/G165Y/L216W (M3)—N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide22.80.2114pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/L216W (parent)—Hydrogen peroxide1.916.40.1183pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/L216W (parent)—Methyl-butyrate4.3122.60.035pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/L216W (parent)—Methyl-pentanoate2.838.30.095pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/L216W (parent)—Methyl-propionate2.7566.20.0048pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsbergvariant T58A/L216W (parent)—N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide33.10.15220.7pH 7.5, 30 °CPMID 23835157Open
subtilisin Carlsberg (sC)Bacillus licheniformisN-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide4190.2331.80 × 10³ calc.pH 8, 25 °CPMID 22326746Open
subtilisin Carlsberg (sC)variant N212GBacillus licheniformisN-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide4750.2272.09 × 10³ calc.pH 8, 25 °CPMID 22326746Open
subtilisin Carlsberg (sC)variant P210ABacillus licheniformisN-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide7150.3252.20 × 10³ calc.pH 8, 25 °CPMID 22326746Open
subtilisin Carlsberg (sC)variant P210GBacillus licheniformisN-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide6380.2782.29 × 10³ calc.pH 8, 25 °CPMID 22326746Open
subtilisin Carlsberg (sC)variant P210G/T211GBacillus licheniformisN-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide6970.292.4 × 10³ calc.pH 8, 25 °CPMID 22326746Open
subtilisin Carlsberg (sC)variant P210G/T211G/N212GBacillus licheniformisN-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide7590.3012.52 × 10³ calc.pH 8, 25 °CPMID 22326746Open
subtilisin Carlsberg (sC)variant T211GBacillus licheniformisN-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide4620.2791.66 × 10³ calc.pH 8, 25 °CPMID 22326746Open
subtilisin EBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide2.890.833.44pH 8.5, 37 °CPMID 8898079Open
subtilisin EBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide2.50.813.1pH 8.5, 37 °CPMID 9930669Open
subtilisin EBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide19.71.0319.1pH 8.5, 37 °CPMID 8898079Open
subtilisin EBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide150.9716pH 8.5, 37 °CPMID 9930669Open
subtilisin EBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide17.61.859.48pH 8.5, 37 °CPMID 8898079Open
subtilisin EBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide1829.3pH 8.5, 37 °CPMID 9930669Open
subtilisin EBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide1.20.294.4pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant G127ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide1.411.790.79pH 8.5, 37 °CPMID 8898079Open
subtilisin Evariant G127ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide2.753.230.85pH 8.5, 37 °CPMID 8898079Open
subtilisin Evariant G127SBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide0.441.790.25pH 8.5, 37 °CPMID 8898079Open
subtilisin Evariant G127SBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide0.573.230.18pH 8.5, 37 °CPMID 8898079Open
subtilisin Evariant G127VBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide0.751.720.44pH 8.5, 37 °CPMID 8898079Open
subtilisin Evariant G127VBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide0.363.330.11pH 8.5, 37 °CPMID 8898079Open
subtilisin Evariant G127VBacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide0.552.630.21pH 8.5, 37 °CPMID 8898079Open
subtilisin Evariant G154ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide3.13.30.96pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant G154ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide2036.6pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant G154ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide6.71.64.3pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant G154ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide1.50.473.1pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant Y-1A/I31L/G127ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide101.76.2pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant Y-1A/I31L/G127ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide313.49.1pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant Y-1A/I31L/G127ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide0.34.60.067pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant Y-1A/I31L/G127ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide0.870.156pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant Y-1A/I31L/G127A/G154ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide6.40.5712pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant Y-1A/I31L/G127A/G154ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide250.8929pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant Y-1A/I31L/G127A/G154ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide0.350.820.62pH 8.5, 37 °CPMID 9930669Open
subtilisin Evariant Y-1A/I31L/G127A/G154ABacillus subtilisN-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide1.70.07822pH 8.5, 37 °CPMID 9930669Open
subtilisin IBTC-3Bacillus subtilisN-acetyl-L-phenylalanine0.0220.850.02630 °CPMID 15649780Open
subtilisin IBTC-3Bacillus subtilisN-acetyl-L-phenylalanine ethyl ester3.40.2215.45pH 8, 30 °CPMID 15649780Open
subtilisin IBTC-3Bacillus subtilisN-acetyl-L-tyrosine0.140.02530 °CPMID 15649780Open
subtilisin IBTC-3Bacillus subtilisN-acetyl-L-tyrosine ethyl ester0.470.371.26pH 8, 30 °CPMID 15649780Open
subtilisin IBTC-3Bacillus subtilisN-benzoyl-L-arginine ethyl ester1.942.10.92pH 8, 30 °CPMID 15649780Open
subtilisin IBTC-3Bacillus subtilisN-benzoyl-L-tyrosine ethyl ester17.70.3945.4pH 8, 30 °CPMID 15649780Open
subtilisin IBTC-3Bacillus subtilisurea-denatured bovine hemoglobin8500.091 × 10⁴30 °CPMID 15649780Open
subtilisin nattokinase (NK)Bacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide9.150.16156.8pH 8, 25 °CPMID 21074529Open
subtilisin nattokinase (NK)variant G34D (propeptide mutation, propeptide numbering; mature subtilisin sequence is wild type)Bacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide0.640.1973.23pH 8, 25 °CPMID 21074529Open
subtilisin nattokinase (NK)variant I11V (propeptide mutation, propeptide numbering; mature subtilisin sequence is wild type)Bacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide3.080.18316.8pH 8, 25 °CPMID 21074529Open
subtilisin nattokinase (NK)variant I30V (propeptide mutation, propeptide numbering; mature subtilisin sequence is wild type)Bacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide9.140.16455.8pH 8, 25 °CPMID 21074529Open
subtilisin nattokinase (NK)variant W106YBacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide1.980.08722.7pH 8, 25 °CPMID 21074529Open
subtilisin nattokinase (NK)variant W113YBacillus subtilis var. nattosuccinyl-Ala-Ala-Pro-Phe-p-nitroanilide3.710.12928.7pH 8, 25 °CPMID 21074529Open