Values CatLog read from this paper

Probing the importance of hydrogen bonds in the active site of the subtilisin nattokinase by site-directed mutagenesis and molecular dynamics simulation

PMID 16411898doi:10.1042/bj20050772Open in Browse

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CatLog read 5 records (15 values) from this paper: nattokinase (subtilisin NK) from Bacillus subtilis var. natto.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.21.62

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
nattokinase (subtilisin NK)	Bacillus subtilis var. natto	succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	52.1	0.196	265.3	pH 8.6, 25 °C	Open record: nattokinase (subtilisin NK), succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
nattokinase (subtilisin NK)variant D60A	Bacillus subtilis var. natto	succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	3.7	0.527	7	pH 8.6, 25 °C	Open record: nattokinase (subtilisin NK) variant D60A, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
nattokinase (subtilisin NK)variant S33A	Bacillus subtilis var. natto	succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	16.5	0.358	46.1	pH 8.6, 25 °C	Open record: nattokinase (subtilisin NK) variant S33A, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
nattokinase (subtilisin NK)variant S62A	Bacillus subtilis var. natto	succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	8.2	0.386	21.2	pH 8.6, 25 °C	Open record: nattokinase (subtilisin NK) variant S62A, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
nattokinase (subtilisin NK)variant T220A	Bacillus subtilis var. natto	succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	6.5	0.432	15	pH 8.6, 25 °C	Open record: nattokinase (subtilisin NK) variant T220A, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide