Values CatLog read from this paper

Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation

PMID 3922976doi:10.1016/s0021-9258(18)88811-1Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 5 records (15 values) from this paper: subtilisin from Bacillus amyloliquefaciens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.21.62

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
subtilisin	Bacillus amyloliquefaciens	N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide	50	0.14	360	pH 8.6, 25 °C	Open record: subtilisin, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide
subtilisinvariant M222A	Bacillus amyloliquefaciens	N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide	40	0.73	50	pH 8.6, 25 °C	Open record: subtilisin variant M222A, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide
subtilisinvariant M222C	Bacillus amyloliquefaciens	N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide	84	0.48	200	pH 8.6, 25 °C	Open record: subtilisin variant M222C, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide
subtilisinvariant M222L	Bacillus amyloliquefaciens	N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide	5	0.26	20	pH 8.6, 25 °C	Open record: subtilisin variant M222L, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide
subtilisinvariant M222S	Bacillus amyloliquefaciens	N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide	27	0.63	40	pH 8.6, 25 °C	Open record: subtilisin variant M222S, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide