Values CatLog read from this paper
Restriction of substrate specificity of subtilisin E by introduction of a side chain into a conserved glycine residue
PMID 8898079doi:10.1016/0014-5793(96)01014-9Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: subtilisin E from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| subtilisin E | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide | 2.89 | 0.83 | 3.44 | pH 8.5, 37 °C | Open record: subtilisin E, N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide |
| subtilisin E | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide | 19.7 | 1.03 | 19.1 | pH 8.5, 37 °C | Open record: subtilisin E, N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide |
| subtilisin E | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide | 17.6 | 1.85 | 9.48 | pH 8.5, 37 °C | Open record: subtilisin E, N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide |
| subtilisin Evariant G127A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide | 1.41 | 1.79 | 0.79 | pH 8.5, 37 °C | Open record: subtilisin E variant G127A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide |
| subtilisin Evariant G127A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide | 2.75 | 3.23 | 0.85 | pH 8.5, 37 °C | Open record: subtilisin E variant G127A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide |
| subtilisin Evariant G127S | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide | 0.44 | 1.79 | 0.25 | pH 8.5, 37 °C | Open record: subtilisin E variant G127S, N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide |
| subtilisin Evariant G127S | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide | 0.57 | 3.23 | 0.18 | pH 8.5, 37 °C | Open record: subtilisin E variant G127S, N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide |
| subtilisin Evariant G127V | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide | 0.75 | 1.72 | 0.44 | pH 8.5, 37 °C | Open record: subtilisin E variant G127V, N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide |
| subtilisin Evariant G127V | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide | 0.36 | 3.33 | 0.11 | pH 8.5, 37 °C | Open record: subtilisin E variant G127V, N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide |
| subtilisin Evariant G127V | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide | 0.55 | 2.63 | 0.21 | pH 8.5, 37 °C | Open record: subtilisin E variant G127V, N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide |