Values CatLog read from this paper
Redirecting catalysis from proteolysis to perhydrolysis in subtilisin Carlsberg
PMID 23835157doi:10.1016/j.jbiotec.2013.06.017Open in Browse
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CatLog read 25 records (75 values) from this paper: subtilisin Carlsberg.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| subtilisin Carlsberg | — | Hydrogen peroxide | 1.1 | 41 | 0.0267 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg, Hydrogen peroxide |
| subtilisin Carlsberg | — | Methyl-butyrate | 2.1 | 117.7 | 0.0183 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg, Methyl-butyrate |
| subtilisin Carlsberg | — | Methyl-pentanoate | 1.2 | 35.2 | 0.0333 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg, Methyl-pentanoate |
| subtilisin Carlsberg | — | Methyl-propionate | 1.1 | 478.2 | 0.0023 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg, Methyl-propionate |
| subtilisin Carlsberg | — | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 172.6 | 0.59 | 292.5 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Carlsbergvariant T58A/G165I/L216W (M2) | — | Hydrogen peroxide | 3.1 | 20.9 | 0.1467 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165I/L216W (M2), Hydrogen peroxide |
| subtilisin Carlsbergvariant T58A/G165I/L216W (M2) | — | Methyl-butyrate | 10.1 | 93.1 | 0.1083 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165I/L216W (M2), Methyl-butyrate |
| subtilisin Carlsbergvariant T58A/G165I/L216W (M2) | — | Methyl-pentanoate | 6.3 | 29.2 | 0.217 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165I/L216W (M2), Methyl-pentanoate |
| subtilisin Carlsbergvariant T58A/G165I/L216W (M2) | — | Methyl-propionate | 3.4 | 255.2 | 0.0133 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165I/L216W (M2), Methyl-propionate |
| subtilisin Carlsbergvariant T58A/G165I/L216W (M2) | — | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 1.7 | 0.54 | 3.1 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165I/L216W (M2), N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Carlsbergvariant T58A/G165L/L216W (M1) | — | Hydrogen peroxide | 2.9 | 18.8 | 0.1533 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165L/L216W (M1), Hydrogen peroxide |
| subtilisin Carlsbergvariant T58A/G165L/L216W (M1) | — | Methyl-butyrate | 11.3 | 64.7 | 0.1733 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165L/L216W (M1), Methyl-butyrate |
| subtilisin Carlsbergvariant T58A/G165L/L216W (M1) | — | Methyl-pentanoate | 6.4 | 33.3 | 0.1917 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165L/L216W (M1), Methyl-pentanoate |
| subtilisin Carlsbergvariant T58A/G165L/L216W (M1) | — | Methyl-propionate | 3 | 265.1 | 0.0115 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165L/L216W (M1), Methyl-propionate |
| subtilisin Carlsbergvariant T58A/G165L/L216W (M1) | — | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 15.1 | 0.42 | 36 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165L/L216W (M1), N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Carlsbergvariant T58A/G165Y/L216W (M3) | — | Hydrogen peroxide | 3.5 | 18.7 | 0.1867 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165Y/L216W (M3), Hydrogen peroxide |
| subtilisin Carlsbergvariant T58A/G165Y/L216W (M3) | — | Methyl-butyrate | 8.8 | 88.8 | 0.0983 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165Y/L216W (M3), Methyl-butyrate |
| subtilisin Carlsbergvariant T58A/G165Y/L216W (M3) | — | Methyl-pentanoate | 3 | 26.3 | 0.115 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165Y/L216W (M3), Methyl-pentanoate |
| subtilisin Carlsbergvariant T58A/G165Y/L216W (M3) | — | Methyl-propionate | 4 | 349.4 | 0.0113 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165Y/L216W (M3), Methyl-propionate |
| subtilisin Carlsbergvariant T58A/G165Y/L216W (M3) | — | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 22.8 | 0.2 | 114 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/G165Y/L216W (M3), N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Carlsbergvariant T58A/L216W (parent) | — | Hydrogen peroxide | 1.9 | 16.4 | 0.1183 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/L216W (parent), Hydrogen peroxide |
| subtilisin Carlsbergvariant T58A/L216W (parent) | — | Methyl-butyrate | 4.3 | 122.6 | 0.035 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/L216W (parent), Methyl-butyrate |
| subtilisin Carlsbergvariant T58A/L216W (parent) | — | Methyl-pentanoate | 2.8 | 38.3 | 0.095 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/L216W (parent), Methyl-pentanoate |
| subtilisin Carlsbergvariant T58A/L216W (parent) | — | Methyl-propionate | 2.7 | 566.2 | 0.0048 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/L216W (parent), Methyl-propionate |
| subtilisin Carlsbergvariant T58A/L216W (parent) | — | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 33.1 | 0.15 | 220.7 | pH 7.5, 30 °C | Open record: subtilisin Carlsberg variant T58A/L216W (parent), N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |