Values CatLog read from this paper
Engineering a substrate-specific cold-adapted subtilisin
PMID 15047911doi:10.1093/protein/gzh019Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: savinase from Bacillus lentus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| savinase | Bacillus lentus | N-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide | 47 | 1.992 | 24 | pH 9, 24 °C | Open record: savinase, N-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide |
| savinase | Bacillus lentus | N-succinyl-Ala-Ala-Val-Ala-p-nitroanilide | 12 | 0.039 | 294 | pH 9, 24 °C | Open record: savinase, N-succinyl-Ala-Ala-Val-Ala-p-nitroanilide |
| savinase | Bacillus lentus | N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide | 185 | 1.156 | 160 | pH 9, 24 °C | Open record: savinase, N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide |
| savinase | Bacillus lentus | N-succinyl-Phe-Ala-Ala-Phe-p-nitroanilide | 90 | 0.223 | 418 | pH 9, 24 °C | Open record: savinase, N-succinyl-Phe-Ala-Ala-Phe-p-nitroanilide |
| savinase | Bacillus lentus | N-succinyl-Tyr-Val-Ala-Asp-p-nitroanilide | 10 | 0.618 | 16 | pH 9, 24 °C | Open record: savinase, N-succinyl-Tyr-Val-Ala-Asp-p-nitroanilide |
| savinasevariant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid) | Bacillus lentus | N-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide | 31 | 0.983 | 30 | pH 9, 24 °C | Open record: savinase variant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid), N-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide |
| savinasevariant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid) | Bacillus lentus | N-succinyl-Ala-Ala-Val-Ala-p-nitroanilide | 10 | 0.053 | 184 | pH 9, 24 °C | Open record: savinase variant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid), N-succinyl-Ala-Ala-Val-Ala-p-nitroanilide |
| savinasevariant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid) | Bacillus lentus | N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide | 403 | 1.926 | 209 | pH 9, 24 °C | Open record: savinase variant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid), N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide |
| savinasevariant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid) | Bacillus lentus | N-succinyl-Phe-Ala-Ala-Phe-p-nitroanilide | 189 | 0.241 | 767 | pH 9, 24 °C | Open record: savinase variant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid), N-succinyl-Phe-Ala-Ala-Phe-p-nitroanilide |
| savinasevariant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid) | Bacillus lentus | N-succinyl-Tyr-Val-Ala-Asp-p-nitroanilide | 4 | 0.746 | 6 | pH 9, 24 °C | Open record: savinase variant V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I (H5 hybrid), N-succinyl-Tyr-Val-Ala-Asp-p-nitroanilide |