Values CatLog read from this paper
Functional analysis of propeptide as an intramolecular chaperone for in vivo folding of subtilisin nattokinase
PMID 21074529doi:10.1016/j.febslet.2010.11.011Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (18 values) from this paper: subtilisin nattokinase (NK) from Bacillus subtilis var. natto.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| subtilisin nattokinase (NK) | Bacillus subtilis var. natto | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 9.15 | 0.161 | 56.8 | pH 8, 25 °C | Open record: subtilisin nattokinase (NK), succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin nattokinase (NK)variant G34D (propeptide mutation, propeptide numbering; mature subtilisin sequence is wild type) | Bacillus subtilis var. natto | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 0.64 | 0.197 | 3.23 | pH 8, 25 °C | Open record: subtilisin nattokinase (NK) variant G34D (propeptide mutation, propeptide numbering; mature subtilisin sequence is wild type), succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin nattokinase (NK)variant I11V (propeptide mutation, propeptide numbering; mature subtilisin sequence is wild type) | Bacillus subtilis var. natto | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 3.08 | 0.183 | 16.8 | pH 8, 25 °C | Open record: subtilisin nattokinase (NK) variant I11V (propeptide mutation, propeptide numbering; mature subtilisin sequence is wild type), succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin nattokinase (NK)variant I30V (propeptide mutation, propeptide numbering; mature subtilisin sequence is wild type) | Bacillus subtilis var. natto | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 9.14 | 0.164 | 55.8 | pH 8, 25 °C | Open record: subtilisin nattokinase (NK) variant I30V (propeptide mutation, propeptide numbering; mature subtilisin sequence is wild type), succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin nattokinase (NK)variant W106Y | Bacillus subtilis var. natto | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 1.98 | 0.087 | 22.7 | pH 8, 25 °C | Open record: subtilisin nattokinase (NK) variant W106Y, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin nattokinase (NK)variant W113Y | Bacillus subtilis var. natto | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 3.71 | 0.129 | 28.7 | pH 8, 25 °C | Open record: subtilisin nattokinase (NK) variant W113Y, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |