CatLogenzyme kinetics catalog

Values CatLog read from this paper

Purification and characterization of Ak.1 protease, a thermostable subtilisin with a disulphide bond in the substrate-binding cleft

PMID 10926860doi:10.1042/bj3500321Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 21 records (62 values) from this paper: Ak.1 protease from Bacillus sp..

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 3.4.21.62

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
Ak.1 proteaseBacillus sp.N-benzyloxycarbonyl-Gly p-nitrophenyl estercontrol: no DTT (disulphide bond intact)1.9 × 10³8.3230pH 7.5, 20 °COpen
Ak.1 proteaseBacillus sp.N-benzyloxycarbonyl-Gly p-nitrophenyl ester+10 mM DTT (disulphide bond reduced)2.5 × 10³8.3300pH 7.5, 20 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Ala-p-nitroanilide2220.28790pH 7.5, 5 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Ala-p-nitroanilide2.2 × 10⁴0.633.5 × 10⁴pH 7.5, 40 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Ala-p-nitroanilide9.7 × 10⁴2.254.3 × 10⁴pH 7.5, 75 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Ala-p-nitroanilidecontrol: no DTT (disulphide bond intact)1.2 × 10³0.323.8 × 10³pH 7.5, 20 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Ala-p-nitroanilide+10 mM DTT (disulphide bond reduced)7500.581.3 × 10³pH 7.5, 20 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-p-nitroanilide3.7 × 10³17220pH 7.5, 75 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-p-nitroanilidecontrol: no DTT (disulphide bond intact)93424pH 7.5, 20 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-p-nitroanilide+10 mM DTT (disulphide bond reduced)936.6714pH 7.5, 20 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide2220.33670pH 7.5, 5 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide8.8 × 10³0.332.7 × 10⁴pH 7.5, 40 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Ala-p-nitroanilide1.3 × 10⁵0.921.4 × 10⁵pH 7.5, 75 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide3890.68570pH 7.5, 5 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide8.8 × 10⁴1.018.7 × 10⁴pH 7.5, 40 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide8.2 × 10⁶3.372.4 × 10⁶pH 7.5, 75 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide1480.072.1 × 10³pH 7.5, 5 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide1.3 × 10⁵0.34.3 × 10⁵pH 7.5, 40 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide1.1 × 10⁷11.1 × 10⁷ calc.pH 7.5, 75 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilidecontrol: no DTT (disulphide bond intact)1 × 10⁴0.156.7 × 10⁴pH 7.5, 20 °COpen
Ak.1 proteaseBacillus sp.N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide+10 mM DTT (disulphide bond reduced)2.9 × 10³0.446.6 × 10³pH 7.5, 20 °COpen