Values CatLog read from this paper
Random mutagenesis into the conserved Gly154 of subtilisin E: isolation and characterization of the revertant enzymes
PMID 9930669doi:10.1093/protein/11.12.1205Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (48 values) from this paper: subtilisin E from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| subtilisin E | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide | 2.5 | 0.81 | 3.1 | pH 8.5, 37 °C | Open record: subtilisin E, N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide |
| subtilisin E | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide | 15 | 0.97 | 16 | pH 8.5, 37 °C | Open record: subtilisin E, N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide |
| subtilisin E | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide | 18 | 2 | 9.3 | pH 8.5, 37 °C | Open record: subtilisin E, N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide |
| subtilisin E | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide | 1.2 | 0.29 | 4.4 | pH 8.5, 37 °C | Open record: subtilisin E, N-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide |
| subtilisin Evariant G154A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide | 3.1 | 3.3 | 0.96 | pH 8.5, 37 °C | Open record: subtilisin E variant G154A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide |
| subtilisin Evariant G154A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide | 20 | 3 | 6.6 | pH 8.5, 37 °C | Open record: subtilisin E variant G154A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide |
| subtilisin Evariant G154A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide | 6.7 | 1.6 | 4.3 | pH 8.5, 37 °C | Open record: subtilisin E variant G154A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide |
| subtilisin Evariant G154A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide | 1.5 | 0.47 | 3.1 | pH 8.5, 37 °C | Open record: subtilisin E variant G154A, N-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide |
| subtilisin Evariant Y-1A/I31L/G127A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide | 10 | 1.7 | 6.2 | pH 8.5, 37 °C | Open record: subtilisin E variant Y-1A/I31L/G127A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide |
| subtilisin Evariant Y-1A/I31L/G127A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide | 31 | 3.4 | 9.1 | pH 8.5, 37 °C | Open record: subtilisin E variant Y-1A/I31L/G127A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide |
| subtilisin Evariant Y-1A/I31L/G127A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide | 0.3 | 4.6 | 0.067 | pH 8.5, 37 °C | Open record: subtilisin E variant Y-1A/I31L/G127A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide |
| subtilisin Evariant Y-1A/I31L/G127A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide | 0.87 | 0.15 | 6 | pH 8.5, 37 °C | Open record: subtilisin E variant Y-1A/I31L/G127A, N-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide |
| subtilisin Evariant Y-1A/I31L/G127A/G154A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide | 6.4 | 0.57 | 12 | pH 8.5, 37 °C | Open record: subtilisin E variant Y-1A/I31L/G127A/G154A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide |
| subtilisin Evariant Y-1A/I31L/G127A/G154A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide | 25 | 0.89 | 29 | pH 8.5, 37 °C | Open record: subtilisin E variant Y-1A/I31L/G127A/G154A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Met-p-nitroanilide |
| subtilisin Evariant Y-1A/I31L/G127A/G154A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide | 0.35 | 0.82 | 0.62 | pH 8.5, 37 °C | Open record: subtilisin E variant Y-1A/I31L/G127A/G154A, N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide |
| subtilisin Evariant Y-1A/I31L/G127A/G154A | Bacillus subtilis | N-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide | 1.7 | 0.078 | 22 | pH 8.5, 37 °C | Open record: subtilisin E variant Y-1A/I31L/G127A/G154A, N-succinyl-L-Ala-L-Ala-L-Val-L-Ala-p-nitroanilide |