Values CatLog read from this paper
Gly or Ala substitutions for Pro(210)Thr(211)Asn(212) at the β8-β9 turn of subtilisin Carlsberg increase the catalytic rate and decrease thermostability
PMID 22326746doi:10.1016/j.bbapap.2012.01.015Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (14 values) from this paper: subtilisin Carlsberg (sC) from Bacillus licheniformis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| subtilisin Carlsberg (sC) | Bacillus licheniformis | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 419 | 0.233 | 1.80 × 10³ calc. | pH 8, 25 °C | Open record: subtilisin Carlsberg (sC), N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Carlsberg (sC)variant N212G | Bacillus licheniformis | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 475 | 0.227 | 2.09 × 10³ calc. | pH 8, 25 °C | Open record: subtilisin Carlsberg (sC) variant N212G, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Carlsberg (sC)variant P210A | Bacillus licheniformis | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 715 | 0.325 | 2.20 × 10³ calc. | pH 8, 25 °C | Open record: subtilisin Carlsberg (sC) variant P210A, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Carlsberg (sC)variant P210G | Bacillus licheniformis | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 638 | 0.278 | 2.29 × 10³ calc. | pH 8, 25 °C | Open record: subtilisin Carlsberg (sC) variant P210G, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Carlsberg (sC)variant P210G/T211G | Bacillus licheniformis | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 697 | 0.29 | 2.4 × 10³ calc. | pH 8, 25 °C | Open record: subtilisin Carlsberg (sC) variant P210G/T211G, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Carlsberg (sC)variant P210G/T211G/N212G | Bacillus licheniformis | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 759 | 0.301 | 2.52 × 10³ calc. | pH 8, 25 °C | Open record: subtilisin Carlsberg (sC) variant P210G/T211G/N212G, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Carlsberg (sC)variant T211G | Bacillus licheniformis | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 462 | 0.279 | 1.66 × 10³ calc. | pH 8, 25 °C | Open record: subtilisin Carlsberg (sC) variant T211G, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |