Values CatLog read from this paper
Toward tailoring the specificity of the S1 pocket of subtilisin B. lentus: chemical modification of mutant enzymes as a strategy for removing specificity limitations
PMID 10529215doi:10.1021/bi990861oOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (63 values) from this paper: subtilisin Bacillus lentus (SBL) from Bacillus lentus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| subtilisin Bacillus lentus (SBL) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide | 17.7 | 2 | 8.8 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL), succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 0.16 | 7.2 | 0.022 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-glutamyl-para-nitroanilide | 1.75 | 4.4 | 0.4 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL), succinyl-alanyl-alanyl-prolyl-glutamyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 153 | 0.73 | 209 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2(CH2)2CH2COO- (S166C-S-h) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 0.35 | 8.2 | 0.041 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2(CH2)2CH2COO- (S166C-S-h), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2(CH2)8CH3 (S166C-S-e) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide | 6.8 | 1.9 | 3.6 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2(CH2)8CH3 (S166C-S-e), succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2(CH2)8CH3 (S166C-S-e) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 82 | 1.09 | 75 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2(CH2)8CH3 (S166C-S-e), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H11 (S166C-S-f) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide | 28.2 | 1.9 | 14.8 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H11 (S166C-S-f), succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H11 (S166C-S-f) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 4.8 | 0.7 | 6.9 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H11 (S166C-S-f), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H4-3,5-(COO-)2 (S166C-S-d) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 0.68 | 5.5 | 0.12 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H4-3,5-(COO-)2 (S166C-S-d), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H4-3,5-(COO-)2 (S166C-S-d) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 47 | 1.6 | 29 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H4-3,5-(COO-)2 (S166C-S-d), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H5 (S166C-S-c) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide | 6.8 | 0.8 | 9 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H5 (S166C-S-c), succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2C6H5 (S166C-S-c) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 23.1 | 1.17 | 20 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2C6H5 (S166C-S-c), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2C(CH3)(COO-)2 (S166C-S-i) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 0.43 | 5.3 | 0.08 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2C(CH3)(COO-)2 (S166C-S-i), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2C(COO-)3 (S166C-S-j) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 1.06 | 5.2 | 0.2 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2C(COO-)3 (S166C-S-j), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2NH3+ (S166C-S-a) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-glutamyl-para-nitroanilide | 14.5 | 1.9 | 7.6 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2NH3+ (S166C-S-a), succinyl-alanyl-alanyl-prolyl-glutamyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2NH3+ (S166C-S-a) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 50 | 0.68 | 74 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2NH3+ (S166C-S-a), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2SO3- (S166C-S-b) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide | 0.17 | 3.4 | 0.05 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2SO3- (S166C-S-b), succinyl-alanyl-alanyl-prolyl-arginyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-CH2CH2SO3- (S166C-S-b) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 25 | 1.34 | 19 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-CH2CH2SO3- (S166C-S-b), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-steroidyl (S166C-S-g) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide | 9.65 | 1.74 | 5.54 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-steroidyl (S166C-S-g), succinyl-alanyl-alanyl-prolyl-alanyl-para-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-steroidyl (S166C-S-g) | Bacillus lentus | succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide | 29 | 0.74 | 41 | pH 8.6, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-steroidyl (S166C-S-g), succinyl-alanyl-alanyl-prolyl-phenylalanyl-para-nitroanilide |