Values CatLog read from this paper
Site-directed mutagenesis combined with chemical modification as a strategy for altering the specificity of the S1 and S1' pockets of subtilisin Bacillus lentus
PMID 9558332doi:10.1021/bi9727951Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 17 records (51 values) from this paper: subtilisin Bacillus lentus (SBL) from Bacillus lentus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| subtilisin Bacillus lentus (SBL)variant M222C-S-b | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 7.2 | 0.63 | 11 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant M222C-S-b, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant M222C-S-c | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 0.49 | 0.69 | 0.71 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant M222C-S-c, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant M222C-S-d | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 6.9 | 0.67 | 10.3 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant M222C-S-d, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 43 | 0.65 | 66 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S156C, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-a | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 30 | 0.79 | 38 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-a, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-b | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 23.4 | 0.68 | 34 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-b, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-c | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 24.2 | 0.6 | 40 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-c, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-d | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 21.8 | 0.54 | 40 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-d, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-e | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 31.6 | 0.78 | 40 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-e, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S156C-S-f | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 39 | 0.86 | 45 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S156C-S-f, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 14.2 | 0.51 | 28 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-a | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 17.4 | 0.39 | 45 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-a, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-b | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 11.8 | 0.92 | 12.8 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-b, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-c | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 31 | 0.72 | 47 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-c, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-d | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 6.9 | 0.74 | 9 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-d, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-e | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 3.8 | 0.7 | 5.4 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-e, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| subtilisin Bacillus lentus (SBL)variant S166C-S-f | Bacillus lentus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 16.3 | 0.6 | 27 | pH 7.5, 25 °C | Open record: subtilisin Bacillus lentus (SBL) variant S166C-S-f, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |