CatLogenzyme kinetics catalog

Values CatLog read from this paper

The hyperthermostable indoleglycerol phosphate synthase from Thermotoga maritima is destabilized by mutational disruption of two solvent-exposed salt bridges

PMID 10329177doi:10.1006/jmbi.1999.2709Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 7 records (19 values) from this paper: indoleglycerol phosphate synthase (tIGPS) from Thermotoga maritima.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.1.1.48

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
indoleglycerol phosphate synthase (tIGPS)Thermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate0.116 × 10⁻⁶1.8 × 10⁴pH 7.5, 25 °COpen
indoleglycerol phosphate synthase (tIGPS)Thermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate0.751.4 × 10⁻⁵5.4 × 10⁴pH 7.5, 45 °COpen
indoleglycerol phosphate synthase (tIGPS)Thermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate1 nM enzyme; fluorometric assay (IGP formation)3.245.3 × 10⁻⁵6.1 × 10⁴pH 7.5, 60 °COpen
indoleglycerol phosphate synthase (tIGPS)Thermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphatein the presence of 1 mM phosphate; fluorometric assay2.51 × 10⁻⁴2.5 × 10⁴pH 7.5, 60 °COpen
indoleglycerol phosphate synthase (tIGPS)Thermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphatein the presence of 1 mM phosphate—2.6 × 10⁻⁴—pH 7.5, 60 °COpen
indoleglycerol phosphate synthase (tIGPS)variant D184AThermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate1.57.4 × 10⁻⁵2 × 10⁴pH 7.5, 60 °COpen
indoleglycerol phosphate synthase (tIGPS)variant R241AThermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate2.51.17 × 10⁻⁴2.1 × 10⁴pH 7.5, 60 °COpen