EC 4.1.1.48: indole-3-glycerol-phosphate synthase

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CatLog read 50 records for EC 4.1.1.48 from 7 papers, for 3 organisms. Names in these papers: indole-3-glycerol phosphate synthase (ssIGPS), indoleglycerol phosphate synthase (tIGPS), IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), indole-3-glycerol phosphate synthase (sIGPS) and 2 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 38 records for EC 4.1.1.48 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.1.1.48 in Browse.

Papers

Papers with records for EC 4.1.1.48
PaperOrganismsRecords
Loop-loop interactions govern multiple steps in indole-3-glycerol phosphate synthase catalysisSulfolobus solfataricus11
Indoleglycerol phosphate synthase-phosphoribosyl anthranilate isomerase: comparison of the bifunctional enzyme from Escherichia coli with engineered monofunctional domainsEscherichia coli10
The hyperthermostable indoleglycerol phosphate synthase from Thermotoga maritima is destabilized by mutational disruption of two solvent-exposed salt bridgesThermotoga maritima7
Differences in the catalytic mechanisms of mesophilic and thermophilic indole-3-glycerol phosphate synthase enzymes at their adaptive temperaturesEscherichia coli, Sulfolobus solfataricus6
Relationship of Catalysis and Active Site Loop Dynamics in the (βα)8-Barrel Enzyme Indole-3-glycerol Phosphate SynthaseSulfolobus solfataricus6
Kinetic mechanism of indole-3-glycerol phosphate synthaseSulfolobus solfataricus5
Functional identification of the general acid and base in the dehydration step of indole-3-glycerol phosphate synthase catalysisSulfolobus solfataricus5

Records

Records for EC 4.1.1.48 read from the paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaperOpen record
IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase)Escherichia coli1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate3.64.2 × 10⁻⁴8.6 × 10³pH 7.5, 25 °CPMID 7727400Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate
IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase)Escherichia coli1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate45.1 × 10⁻⁴7.8 × 10³pH 7.8, 25 °CPMID 7727400Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate
IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase)Escherichia coli1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphateinitial-velocity analysis3.244.2 × 10⁻⁴7.7 × 10³ calc.pH 7.5, 25 °CPMID 7727400Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate
IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase)Escherichia coli1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate1.2 µM CdRP; Figure 23.23.2 × 10⁻⁴1.0 × 10⁴ calc.pH 7.5, 25 °CPMID 7727400Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate
IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase)Escherichia coli1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphateFigure 3—5.1 × 10⁻⁴—pH 7.5, 25 °CPMID 7727400Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate
indole-3-glycerol phosphate synthase (ecIGPS)Escherichia coli1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate4.1——pH 7.5, 25 °CPMID 22274606Open record: indole-3-glycerol phosphate synthase (ecIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ecIGPS)Escherichia coli1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate9.30.00165.7 × 10³pH 7.5, 37 °CPMID 22274606Open record: indole-3-glycerol phosphate synthase (ecIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (sIGPS)Sulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.0625.1 × 10⁻⁵1.2 × 10³pH 7.5, 25 °CPMID 23214473Open record: indole-3-glycerol phosphate synthase (sIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (sIGPS)variant D61CSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphateAlexa Fluor 5550.0384.6 × 10⁻⁵830pH 7.5, 25 °CPMID 23214473Open record: indole-3-glycerol phosphate synthase (sIGPS) variant D61C, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (sIGPS)variant D61CSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphatePyMPO0.0651.09 × 10⁻⁴600pH 7.5, 25 °CPMID 23214473Open record: indole-3-glycerol phosphate synthase (sIGPS) variant D61C, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (sIGPS)variant R18CSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphateAlexa Fluor 5550.0425 × 10⁻⁵840pH 7.5, 25 °CPMID 23214473Open record: indole-3-glycerol phosphate synthase (sIGPS) variant R18C, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (sIGPS)variant R18CSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphatePyMPO0.044.5 × 10⁻⁵890pH 7.5, 25 °CPMID 23214473Open record: indole-3-glycerol phosphate synthase (sIGPS) variant R18C, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)Sulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.167.4 × 10⁻⁵2.2 × 10³pH 7.5, 25 °CPMID 22274606Open record: indole-3-glycerol phosphate synthase (ssIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)Sulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.428.8 × 10⁻⁵4.8 × 10³pH 7.5, 37 °CPMID 22274606Open record: indole-3-glycerol phosphate synthase (ssIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)Sulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.518.8 × 10⁻⁵5.8 × 10³pH 7.5, 50 °CPMID 22274606Open record: indole-3-glycerol phosphate synthase (ssIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)Sulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.674.4 × 10⁻⁵1.5 × 10⁴pH 7.5, 75 °CPMID 22274606Open record: indole-3-glycerol phosphate synthase (ssIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)Sulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.118.5 × 10⁻⁵1.18 × 10³pH 7.5, 25 °CPMID 29498826Open record: indole-3-glycerol phosphate synthase (ssIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)Sulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate2.11.05 × 10⁻⁴2 × 10⁴pH 7.5, 60 °CPMID 29498826Open record: indole-3-glycerol phosphate synthase (ssIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant E210QSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.973.86 × 10⁻⁴2.5 × 10³pH 7.5, 75 °CPMID 23900843Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant E210Q, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant E51QSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.0071.3 × 10⁻⁵540pH 7.5, 75 °CPMID 23900843Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant E51Q, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant F246SSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.320.0013240pH 7.5, 25 °CPMID 29498826Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant F246S, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant F89ASulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.060.00125075 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant F89A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant G212ESulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.160.02177.4 calc.pH 7.5, 25 °CPMID 29498826Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant G212E, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant K53QSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.001——pH 7.5, 75 °CPMID 23900843Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant K53Q, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant K53RSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.060.0024430pH 7.5, 75 °CPMID 23900843Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant K53R, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant L236QSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.20.003460pH 7.5, 25 °CPMID 29498826Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant L236Q, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant M237TSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.230.002980pH 7.5, 25 °CPMID 29498826Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant M237T, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant N90ASulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.043.6 × 10⁻⁵1 × 10³25 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant N90A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant N90ASulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.177.4 × 10⁻⁵2.3 × 10³37 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant N90A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant N90ASulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.557.9 × 10⁻⁵7 × 10³75 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant N90A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant N90QSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.31.32 × 10⁻⁴2.3 × 10³37 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant N90Q, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant N90QSulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.891.17 × 10⁻⁴7.6 × 10³75 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant N90Q, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant R182ASulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.410.001062380pH 7.5, 75 °CPMID 23900843Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant R182A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant R54ASulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.161.45 × 10⁻⁴2.2 × 10³25 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant R54A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant R54ASulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.359.5 × 10⁻⁵3.7 × 10³37 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant R54A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant R54ASulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate1.27.4 × 10⁻⁵1.6 × 10⁴75 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant R54A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant R54A/N90ASulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.192.3 × 10⁻⁵8.2 × 10³37 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant R54A/N90A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indole-3-glycerol phosphate synthase (ssIGPS)variant R54A/N90ASulfolobus solfataricus1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate0.511.07 × 10⁻⁴4.8 × 10³75 °CPMID 24403092Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant R54A/N90A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate
indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI)Escherichia coli1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate3.24.7 × 10⁻⁴6.8 × 10³pH 7.5, 25 °CPMID 7727400Open record: indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate
indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI)Escherichia coli1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphateinitial-velocity analysis2.44.6 × 10⁻⁴5.2 × 10³ calc.pH 7.5, 25 °CPMID 7727400Open record: indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate
indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI)Escherichia coli1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate1.2 µM CdRP; Figure 22.54.4 × 10⁻⁴5.7 × 10³ calc.pH 7.5, 25 °CPMID 7727400Open record: indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate
indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI)Escherichia coli1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphateFigure 3—5.4 × 10⁻⁴—pH 7.5, 25 °CPMID 7727400Open record: indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate
indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI)variant L253F/L254A (IGPS[1-252FAG])Escherichia coli1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate4.30.013330pH 7.8, 25 °CPMID 7727400Open record: indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI) variant L253F/L254A (IGPS[1-252FAG]), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate
indoleglycerol phosphate synthase (tIGPS)Thermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate0.116 × 10⁻⁶1.8 × 10⁴pH 7.5, 25 °CPMID 10329177Open record: indoleglycerol phosphate synthase (tIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate
indoleglycerol phosphate synthase (tIGPS)Thermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate0.751.4 × 10⁻⁵5.4 × 10⁴pH 7.5, 45 °CPMID 10329177Open record: indoleglycerol phosphate synthase (tIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate
indoleglycerol phosphate synthase (tIGPS)Thermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate1 nM enzyme; fluorometric assay (IGP formation)3.245.3 × 10⁻⁵6.1 × 10⁴pH 7.5, 60 °CPMID 10329177Open record: indoleglycerol phosphate synthase (tIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate
indoleglycerol phosphate synthase (tIGPS)Thermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphatein the presence of 1 mM phosphate; fluorometric assay2.51 × 10⁻⁴2.5 × 10⁴pH 7.5, 60 °CPMID 10329177Open record: indoleglycerol phosphate synthase (tIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate
indoleglycerol phosphate synthase (tIGPS)Thermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphatein the presence of 1 mM phosphate—2.6 × 10⁻⁴—pH 7.5, 60 °CPMID 10329177Open record: indoleglycerol phosphate synthase (tIGPS), 1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate
indoleglycerol phosphate synthase (tIGPS)variant D184AThermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate1.57.4 × 10⁻⁵2 × 10⁴pH 7.5, 60 °CPMID 10329177Open record: indoleglycerol phosphate synthase (tIGPS) variant D184A, 1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate
indoleglycerol phosphate synthase (tIGPS)variant R241AThermotoga maritima1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate2.51.17 × 10⁻⁴2.1 × 10⁴pH 7.5, 60 °CPMID 10329177Open record: indoleglycerol phosphate synthase (tIGPS) variant R241A, 1-(o-carboxyphenylamino)-1-deoxyribulose-5-phosphate