Values CatLog read from this paper
Functional identification of the general acid and base in the dehydration step of indole-3-glycerol phosphate synthase catalysis
PMID 23900843doi:10.1074/jbc.m113.487447Open in Browse
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CatLog read 5 records (13 values) from this paper: indole-3-glycerol phosphate synthase (ssIGPS) from Sulfolobus solfataricus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| indole-3-glycerol phosphate synthase (ssIGPS)variant E210Q | Sulfolobus solfataricus | 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate | 0.97 | 3.86 × 10⁻⁴ | 2.5 × 10³ | pH 7.5, 75 °C | Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant E210Q, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate |
| indole-3-glycerol phosphate synthase (ssIGPS)variant E51Q | Sulfolobus solfataricus | 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate | 0.007 | 1.3 × 10⁻⁵ | 540 | pH 7.5, 75 °C | Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant E51Q, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate |
| indole-3-glycerol phosphate synthase (ssIGPS)variant K53Q | Sulfolobus solfataricus | 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate | 0.001 | — | — | pH 7.5, 75 °C | Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant K53Q, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate |
| indole-3-glycerol phosphate synthase (ssIGPS)variant K53R | Sulfolobus solfataricus | 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate | 0.06 | 0.00244 | 30 | pH 7.5, 75 °C | Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant K53R, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate |
| indole-3-glycerol phosphate synthase (ssIGPS)variant R182A | Sulfolobus solfataricus | 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate | 0.41 | 0.001062 | 380 | pH 7.5, 75 °C | Open record: indole-3-glycerol phosphate synthase (ssIGPS) variant R182A, 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate |