Values CatLog read from this paper
Indoleglycerol phosphate synthase-phosphoribosyl anthranilate isomerase: comparison of the bifunctional enzyme from Escherichia coli with engineered monofunctional domains
PMID 7727400doi:10.1021/bi00016a013Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (28 values) from this paper: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI), phosphoribosyl anthranilate isomerase (monofunctional PRAI domain of IGPS:PRAI) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase) | Escherichia coli | 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate | 3.6 | 4.2 × 10⁻⁴ | 8.6 × 10³ | pH 7.5, 25 °C | Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate |
| IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase) | Escherichia coli | 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate | 4 | 5.1 × 10⁻⁴ | 7.8 × 10³ | pH 7.8, 25 °C | Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate |
| IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase) | Escherichia coli | 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphateinitial-velocity analysis | 3.24 | 4.2 × 10⁻⁴ | 7.7 × 10³ calc. | pH 7.5, 25 °C | Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate |
| IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase) | Escherichia coli | 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate1.2 µM CdRP; Figure 2 | 3.2 | 3.2 × 10⁻⁴ | 1.0 × 10⁴ calc. | pH 7.5, 25 °C | Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate |
| IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase) | Escherichia coli | 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphateFigure 3 | — | 5.1 × 10⁻⁴ | — | pH 7.5, 25 °C | Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate |
| IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase) | Escherichia coli | N-(5-phospho-beta-D-ribosyl)anthranilate | 40 | 0.0049 | 8.2 × 10³ | pH 7.5, 25 °C | Open record: IGPS:PRAI (bifunctional indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase), N-(5-phospho-beta-D-ribosyl)anthranilate |
| indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI) | Escherichia coli | 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate | 3.2 | 4.7 × 10⁻⁴ | 6.8 × 10³ | pH 7.5, 25 °C | Open record: indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate |
| indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI) | Escherichia coli | 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphateinitial-velocity analysis | 2.4 | 4.6 × 10⁻⁴ | 5.2 × 10³ calc. | pH 7.5, 25 °C | Open record: indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate |
| indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI) | Escherichia coli | 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate1.2 µM CdRP; Figure 2 | 2.5 | 4.4 × 10⁻⁴ | 5.7 × 10³ calc. | pH 7.5, 25 °C | Open record: indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate |
| indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI) | Escherichia coli | 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphateFigure 3 | — | 5.4 × 10⁻⁴ | — | pH 7.5, 25 °C | Open record: indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate |
| indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI)variant L253F/L254A (IGPS[1-252FAG]) | Escherichia coli | 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate | 4.3 | 0.013 | 330 | pH 7.8, 25 °C | Open record: indoleglycerol phosphate synthase (monofunctional IGPS domain of IGPS:PRAI) variant L253F/L254A (IGPS[1-252FAG]), 1-[(2-carboxyphenyl)amino]-1-deoxy-D-ribulose 5-phosphate |
| phosphoribosyl anthranilate isomerase (monofunctional PRAI domain of IGPS:PRAI)variant V330A (PRAI[ML256-452]) | Escherichia coli | N-(5-phospho-beta-D-ribosyl)anthranilate | 32 | 0.0047 | 6.8 × 10³ | pH 7.5, 25 °C | Open record: phosphoribosyl anthranilate isomerase (monofunctional PRAI domain of IGPS:PRAI) variant V330A (PRAI[ML256-452]), N-(5-phospho-beta-D-ribosyl)anthranilate |