Values CatLog read from this paper
Identification and designing of the S3 site of aqualysin I, a thermophilic subtilisin-related serine protease
PMID 10348901doi:10.1093/oxfordjournals.jbchem.a022380Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (52 values) from this paper: aqualysin I from Thermus aquaticus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| aqualysin Ivariant G131D | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 2.4 | 2.1 | 1.1 | pH 7.5, 40 °C | Open record: aqualysin I variant G131D, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G131D | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 7.9 | 0.49 | 16 | pH 7.5, 40 °C | Open record: aqualysin I variant G131D, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G131D | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | — | — | 13 | pH 7.5, 40 °C | Open record: aqualysin I variant G131D, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G131H | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 7.3 | 7.1 | 1 | pH 7.5, 40 °C | Open record: aqualysin I variant G131H, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G131H | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 8.9 | 1.9 | 4.7 | pH 7.5, 40 °C | Open record: aqualysin I variant G131H, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G131H | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 1.1 | 0.52 | 2.1 | pH 7.5, 40 °C | Open record: aqualysin I variant G131H, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G131K | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 5.4 | 2.3 | 2.4 | pH 7.5, 40 °C | Open record: aqualysin I variant G131K, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G131K | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 9 | 2 | 4.4 | pH 7.5, 40 °C | Open record: aqualysin I variant G131K, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G131K | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 1.5 | 0.51 | 3 | pH 7.5, 40 °C | Open record: aqualysin I variant G131K, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102E | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 0.5 | 3.8 | 0.13 | pH 7.5, 40 °C | Open record: aqualysin I variant S102E, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102E | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 25 | 1.1 | 24 | pH 7.5, 40 °C | Open record: aqualysin I variant S102E, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant S102E | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 0.11 | 0.34 | 0.33 | pH 7.5, 40 °C | Open record: aqualysin I variant S102E, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102H | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 1.6 | 2.1 | 0.76 | pH 7.5, 40 °C | Open record: aqualysin I variant S102H, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102H | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 54 | 1.8 | 30 | pH 7.5, 40 °C | Open record: aqualysin I variant S102H, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant S102H | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 0.11 | 0.24 | 0.45 | pH 7.5, 40 °C | Open record: aqualysin I variant S102H, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102K | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 8.5 | 10 | 0.83 | pH 7.5, 40 °C | Open record: aqualysin I variant S102K, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102K | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 145 | 1.7 | 85 | pH 7.5, 40 °C | Open record: aqualysin I variant S102K, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant S102K | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 2.9 | 0.15 | 20 | pH 7.5, 40 °C | Open record: aqualysin I variant S102K, succinyl-Phe-Ala-Ala-p-nitroanilide |