EC 3.4.21.111: aqualysin 1
HydrolasesOpen in Browse
CatLog read 53 records for EC 3.4.21.111 from 4 papers, for 1 organism. Names in these papers: aqualysin I, Aqualysin I (AQN).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 11 records for EC 3.4.21.111 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.111 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Identification and designing of the S3 site of aqualysin I, a thermophilic subtilisin-related serine protease | Thermus aquaticus | 18 |
| Engineering of S2 site of aqualysin I; alteration of P2 specificity by excluding P2 side chain | Thermus aquaticus | 13 |
| Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1: Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg | Thermus aquaticus | 12 |
| Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1 | Thermus aquaticus | 10 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| aqualysin I | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 1.4 | 1.3 | 1.1 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Ala-Ala-Phe-p-nitroanilide | 2.5 | 0.91 | 2.8 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Ala-Ala-Phe-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 33 | 1.2 | 27 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Ala-Ala-Val-p-nitroanilide | 0.047 | 1.6 | 0.029 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Ala-Ala-Val-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Ala-Pro-Ala-p-nitroanilide | 0.48 | 0.79 | 0.61 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Ala-Pro-Ala-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Gly-Gly-Phe-p-nitroanilide | 0.15 | 1.6 | 0.095 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Gly-Gly-Phe-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Ile-Val-Ala-p-nitroanilide | 5 | 0.064 | 78 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Ile-Val-Ala-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 10 | 0.04 | 260 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Phe-Leu-Ala-p-nitroanilide | 3.6 | 0.087 | 41 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Phe-Leu-Ala-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Phe-Nle-Ala-p-nitroanilide | 8.8 | 0.036 | 240 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Phe-Nle-Ala-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Phe-Val-Ala-p-nitroanilide | 4.7 | 0.039 | 120 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Phe-Val-Ala-p-nitroanilide |
| aqualysin I | Thermus aquaticus | succinyl-Phe-Val-Leu-p-nitroanilide | 0.069 | 0.41 | 0.17 | pH 7.5, 40 °C | PMID 27393587 | Open record: aqualysin I, succinyl-Phe-Val-Leu-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Ala-Ala-Phe-p-nitroanilide | 0.22 | 1.7 | 0.13 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101A, succinyl-Ala-Ala-Phe-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 0.88 | 1.3 | 0.68 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101A, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Gly-Gly-Phe-p-nitroanilide | 0.17 | 4.2 | 0.04 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101A, succinyl-Gly-Gly-Phe-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | — | — | 0.0065 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101A, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Phe-Leu-Ala-p-nitroanilide | — | — | 0.027 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101A, succinyl-Phe-Leu-Ala-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Phe-Nle-Ala-p-nitroanilide | 0.18 | 0.73 | 0.25 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101A, succinyl-Phe-Nle-Ala-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Phe-Val-Ala-p-nitroanilide | 0.18 | 1.2 | 0.15 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101A, succinyl-Phe-Val-Ala-p-nitroanilide |
| aqualysin Ivariant G101L | Thermus aquaticus | succinyl-Ala-Ala-Phe-p-nitroanilide | 0.009 | 1.5 | 0.0062 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101L, succinyl-Ala-Ala-Phe-p-nitroanilide |
| aqualysin Ivariant G101L | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 0.014 | 0.67 | 0.021 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101L, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G101L | Thermus aquaticus | succinyl-Gly-Gly-Phe-p-nitroanilide | 0.024 | 5.3 | 0.0045 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101L, succinyl-Gly-Gly-Phe-p-nitroanilide |
| aqualysin Ivariant G101V | Thermus aquaticus | succinyl-Ala-Ala-Phe-p-nitroanilide | 0.091 | 1 | 0.09 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101V, succinyl-Ala-Ala-Phe-p-nitroanilide |
| aqualysin Ivariant G101V | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 0.9 | 0.73 | 1.2 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101V, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G101V | Thermus aquaticus | succinyl-Gly-Gly-Phe-p-nitroanilide | 0.25 | 3.4 | 0.072 | pH 7.5, 40 °C | PMID 9860855 | Open record: aqualysin I variant G101V, succinyl-Gly-Gly-Phe-p-nitroanilide |
| aqualysin Ivariant G131D | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 2.4 | 2.1 | 1.1 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant G131D, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G131D | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 7.9 | 0.49 | 16 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant G131D, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G131D | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | — | — | 13 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant G131D, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G131H | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 7.3 | 7.1 | 1 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant G131H, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G131H | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 8.9 | 1.9 | 4.7 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant G131H, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G131H | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 1.1 | 0.52 | 2.1 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant G131H, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G131K | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 5.4 | 2.3 | 2.4 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant G131K, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G131K | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 9 | 2 | 4.4 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant G131K, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G131K | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 1.5 | 0.51 | 3 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant G131K, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102E | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 0.5 | 3.8 | 0.13 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant S102E, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102E | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 25 | 1.1 | 24 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant S102E, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant S102E | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 0.11 | 0.34 | 0.33 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant S102E, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102H | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 1.6 | 2.1 | 0.76 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant S102H, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102H | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 54 | 1.8 | 30 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant S102H, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant S102H | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 0.11 | 0.24 | 0.45 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant S102H, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102K | Thermus aquaticus | succinyl-Ala-Ala-Ala-p-nitroanilide | 8.5 | 10 | 0.83 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant S102K, succinyl-Ala-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant S102K | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 145 | 1.7 | 85 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant S102K, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant S102K | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | 2.9 | 0.15 | 20 | pH 7.5, 40 °C | PMID 10348901 | Open record: aqualysin I variant S102K, succinyl-Phe-Ala-Ala-p-nitroanilide |
| Aqualysin I (AQN) | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 91.6 | 0.79 | 116 | pH 7.5, 40 °C | PMID 25136511 | Open record: Aqualysin I (AQN), N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant D138N | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 59.5 | 0.77 | 77.3 | pH 7.5, 40 °C | PMID 25136511 | Open record: Aqualysin I (AQN) variant D138N, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant D17N | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 110 | 0.98 | 113 | pH 7.5, 40 °C | PMID 25136511 | Open record: Aqualysin I (AQN) variant D17N, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant D183N | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 53 | 0.74 | 71.6 | pH 7.5, 40 °C | PMID 25136511 | Open record: Aqualysin I (AQN) variant D183N, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant D212N | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 75.2 | 1.1 | 68.6 | pH 7.5, 40 °C | PMID 25136511 | Open record: Aqualysin I (AQN) variant D212N, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant D58N | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 68.5 | 0.99 | 70 | pH 7.5, 40 °C | PMID 25136511 | Open record: Aqualysin I (AQN) variant D58N, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant E237Q | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 96.1 | 0.91 | 105 | pH 7.5, 40 °C | PMID 25136511 | Open record: Aqualysin I (AQN) variant E237Q, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant G262D | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 61.3 | 0.93 | 66.2 | pH 7.5, 40 °C | PMID 25136511 | Open record: Aqualysin I (AQN) variant G262D, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant G61D | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 47.2 | 0.82 | 63.6 | pH 7.5, 40 °C | PMID 25136511 | Open record: Aqualysin I (AQN) variant G61D, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant S277D | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 73.6 | 0.8 | 92.5 | pH 7.5, 40 °C | PMID 25136511 | Open record: Aqualysin I (AQN) variant S277D, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |