Values CatLog read from this paper
Engineering of S2 site of aqualysin I; alteration of P2 specificity by excluding P2 side chain
PMID 9860855doi:10.1021/bi981533bOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (35 values) from this paper: aqualysin I from Thermus aquaticus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Ala-Ala-Phe-p-nitroanilide | 0.22 | 1.7 | 0.13 | pH 7.5, 40 °C | Open record: aqualysin I variant G101A, succinyl-Ala-Ala-Phe-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 0.88 | 1.3 | 0.68 | pH 7.5, 40 °C | Open record: aqualysin I variant G101A, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Gly-Gly-Phe-p-nitroanilide | 0.17 | 4.2 | 0.04 | pH 7.5, 40 °C | Open record: aqualysin I variant G101A, succinyl-Gly-Gly-Phe-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Phe-Ala-Ala-p-nitroanilide | — | — | 0.0065 | pH 7.5, 40 °C | Open record: aqualysin I variant G101A, succinyl-Phe-Ala-Ala-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Phe-Leu-Ala-p-nitroanilide | — | — | 0.027 | pH 7.5, 40 °C | Open record: aqualysin I variant G101A, succinyl-Phe-Leu-Ala-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Phe-Nle-Ala-p-nitroanilide | 0.18 | 0.73 | 0.25 | pH 7.5, 40 °C | Open record: aqualysin I variant G101A, succinyl-Phe-Nle-Ala-p-nitroanilide |
| aqualysin Ivariant G101A | Thermus aquaticus | succinyl-Phe-Val-Ala-p-nitroanilide | 0.18 | 1.2 | 0.15 | pH 7.5, 40 °C | Open record: aqualysin I variant G101A, succinyl-Phe-Val-Ala-p-nitroanilide |
| aqualysin Ivariant G101L | Thermus aquaticus | succinyl-Ala-Ala-Phe-p-nitroanilide | 0.009 | 1.5 | 0.0062 | pH 7.5, 40 °C | Open record: aqualysin I variant G101L, succinyl-Ala-Ala-Phe-p-nitroanilide |
| aqualysin Ivariant G101L | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 0.014 | 0.67 | 0.021 | pH 7.5, 40 °C | Open record: aqualysin I variant G101L, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G101L | Thermus aquaticus | succinyl-Gly-Gly-Phe-p-nitroanilide | 0.024 | 5.3 | 0.0045 | pH 7.5, 40 °C | Open record: aqualysin I variant G101L, succinyl-Gly-Gly-Phe-p-nitroanilide |
| aqualysin Ivariant G101V | Thermus aquaticus | succinyl-Ala-Ala-Phe-p-nitroanilide | 0.091 | 1 | 0.09 | pH 7.5, 40 °C | Open record: aqualysin I variant G101V, succinyl-Ala-Ala-Phe-p-nitroanilide |
| aqualysin Ivariant G101V | Thermus aquaticus | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 0.9 | 0.73 | 1.2 | pH 7.5, 40 °C | Open record: aqualysin I variant G101V, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| aqualysin Ivariant G101V | Thermus aquaticus | succinyl-Gly-Gly-Phe-p-nitroanilide | 0.25 | 3.4 | 0.072 | pH 7.5, 40 °C | Open record: aqualysin I variant G101V, succinyl-Gly-Gly-Phe-p-nitroanilide |