Values CatLog read from this paper

Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1: Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg

PMID 27393587doi:10.1271/bbb.62.2161Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 38 records (114 values) from this paper: aqualysin I, proteinase K, subtilisin Carlsberg and 1 more from Thermus aquaticus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.21.111

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
aqualysin I	Thermus aquaticus	succinyl-Ala-Ala-Ala-p-nitroanilide	1.4	1.3	1.1	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Ala-Ala-Ala-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Ala-Ala-Phe-p-nitroanilide	2.5	0.91	2.8	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Ala-Ala-Phe-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	33	1.2	27	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Ala-Ala-Val-p-nitroanilide	0.047	1.6	0.029	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Ala-Ala-Val-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Ala-Pro-Ala-p-nitroanilide	0.48	0.79	0.61	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Ala-Pro-Ala-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Gly-Gly-Phe-p-nitroanilide	0.15	1.6	0.095	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Gly-Gly-Phe-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Ile-Val-Ala-p-nitroanilide	5	0.064	78	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Ile-Val-Ala-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Phe-Ala-Ala-p-nitroanilide	10	0.04	260	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Phe-Ala-Ala-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Phe-Leu-Ala-p-nitroanilide	3.6	0.087	41	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Phe-Leu-Ala-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Phe-Nle-Ala-p-nitroanilide	8.8	0.036	240	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Phe-Nle-Ala-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Phe-Val-Ala-p-nitroanilide	4.7	0.039	120	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Phe-Val-Ala-p-nitroanilide
aqualysin I	Thermus aquaticus	succinyl-Phe-Val-Leu-p-nitroanilide	0.069	0.41	0.17	pH 7.5, 40 °C	Open record: aqualysin I, succinyl-Phe-Val-Leu-p-nitroanilide
proteinase K	—	succinyl-Ala-Ala-Ala-p-nitroanilide	2.7	0.2	13	pH 7.5, 40 °C	Open record: proteinase K, succinyl-Ala-Ala-Ala-p-nitroanilide
proteinase K	—	succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	1.1 × 10⁴	0.34	3.2 × 10⁴	pH 7.5, 40 °C	Open record: proteinase K, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
proteinase K	—	succinyl-Ala-Ala-Val-p-nitroanilide	0.006	0.18	0.033	pH 7.5, 40 °C	Open record: proteinase K, succinyl-Ala-Ala-Val-p-nitroanilide
proteinase K	—	succinyl-Ile-Val-Ala-p-nitroanilide	7.3	0.34	21	pH 7.5, 40 °C	Open record: proteinase K, succinyl-Ile-Val-Ala-p-nitroanilide
proteinase K	—	succinyl-Phe-Ala-Ala-p-nitroanilide	11	0.055	210	pH 7.5, 40 °C	Open record: proteinase K, succinyl-Phe-Ala-Ala-p-nitroanilide
proteinase K	—	succinyl-Phe-Leu-Ala-p-nitroanilide	2.4	0.13	18	pH 7.5, 40 °C	Open record: proteinase K, succinyl-Phe-Leu-Ala-p-nitroanilide
proteinase K	—	succinyl-Phe-Nle-Ala-p-nitroanilide	7.9	0.06	130	pH 7.5, 40 °C	Open record: proteinase K, succinyl-Phe-Nle-Ala-p-nitroanilide
proteinase K	—	succinyl-Phe-Val-Ala-p-nitroanilide	2	0.76	27	pH 7.5, 40 °C	Open record: proteinase K, succinyl-Phe-Val-Ala-p-nitroanilide
proteinase K	—	succinyl-Phe-Val-Leu-p-nitroanilide	0.6	0.35	1.7	pH 7.5, 40 °C	Open record: proteinase K, succinyl-Phe-Val-Leu-p-nitroanilide
subtilisin BPN'	—	succinyl-Ala-Ala-Ala-p-nitroanilide	2.1	3.2	0.67	pH 7.5, 40 °C	Open record: subtilisin BPN', succinyl-Ala-Ala-Ala-p-nitroanilide
subtilisin BPN'	—	succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	480	0.29	1.7 × 10³	pH 7.5, 40 °C	Open record: subtilisin BPN', succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
subtilisin BPN'	—	succinyl-Ala-Ala-Val-p-nitroanilide	0.022	2.4	0.009	pH 7.5, 40 °C	Open record: subtilisin BPN', succinyl-Ala-Ala-Val-p-nitroanilide
subtilisin BPN'	—	succinyl-Phe-Ala-Ala-p-nitroanilide	9.6	0.051	190	pH 7.5, 40 °C	Open record: subtilisin BPN', succinyl-Phe-Ala-Ala-p-nitroanilide
subtilisin BPN'	—	succinyl-Phe-Leu-Ala-p-nitroanilide	3.1	0.25	12	pH 7.5, 40 °C	Open record: subtilisin BPN', succinyl-Phe-Leu-Ala-p-nitroanilide
subtilisin BPN'	—	succinyl-Phe-Nle-Ala-p-nitroanilide	3.3	0.05	660	pH 7.5, 40 °C	Open record: subtilisin BPN', succinyl-Phe-Nle-Ala-p-nitroanilide
subtilisin BPN'	—	succinyl-Phe-Val-Ala-p-nitroanilide	3.7	0.16	24	pH 7.5, 40 °C	Open record: subtilisin BPN', succinyl-Phe-Val-Ala-p-nitroanilide
subtilisin BPN'	—	succinyl-Phe-Val-Leu-p-nitroanilide	0.79	0.07	11	pH 7.5, 40 °C	Open record: subtilisin BPN', succinyl-Phe-Val-Leu-p-nitroanilide
subtilisin Carlsberg	—	succinyl-Ala-Ala-Ala-p-nitroanilide	5.5	0.77	7.1	pH 7.5, 40 °C	Open record: subtilisin Carlsberg, succinyl-Ala-Ala-Ala-p-nitroanilide
subtilisin Carlsberg	—	succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	300	0.29	1 × 10³	pH 7.5, 40 °C	Open record: subtilisin Carlsberg, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
subtilisin Carlsberg	—	succinyl-Ala-Ala-Val-p-nitroanilide	0.16	1.5	0.11	pH 7.5, 40 °C	Open record: subtilisin Carlsberg, succinyl-Ala-Ala-Val-p-nitroanilide
subtilisin Carlsberg	—	succinyl-Ile-Val-Ala-p-nitroanilide	40	0.54	75	pH 7.5, 40 °C	Open record: subtilisin Carlsberg, succinyl-Ile-Val-Ala-p-nitroanilide
subtilisin Carlsberg	—	succinyl-Phe-Ala-Ala-p-nitroanilide	2.3	0.16	14	pH 7.5, 40 °C	Open record: subtilisin Carlsberg, succinyl-Phe-Ala-Ala-p-nitroanilide
subtilisin Carlsberg	—	succinyl-Phe-Leu-Ala-p-nitroanilide	20	0.27	74	pH 7.5, 40 °C	Open record: subtilisin Carlsberg, succinyl-Phe-Leu-Ala-p-nitroanilide
subtilisin Carlsberg	—	succinyl-Phe-Nle-Ala-p-nitroanilide	28	0.024	1.2 × 10³	pH 7.5, 40 °C	Open record: subtilisin Carlsberg, succinyl-Phe-Nle-Ala-p-nitroanilide
subtilisin Carlsberg	—	succinyl-Phe-Val-Ala-p-nitroanilide	12	0.079	150	pH 7.5, 40 °C	Open record: subtilisin Carlsberg, succinyl-Phe-Val-Ala-p-nitroanilide
subtilisin Carlsberg	—	succinyl-Phe-Val-Leu-p-nitroanilide	6.1	0.11	57	pH 7.5, 40 °C	Open record: subtilisin Carlsberg, succinyl-Phe-Val-Leu-p-nitroanilide