Values CatLog read from this paper
Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1
PMID 25136511doi:10.1186/s13568-014-0059-2Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: Aqualysin I (AQN) from Thermus aquaticus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Aqualysin I (AQN) | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 91.6 | 0.79 | 116 | pH 7.5, 40 °C | Open record: Aqualysin I (AQN), N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant D138N | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 59.5 | 0.77 | 77.3 | pH 7.5, 40 °C | Open record: Aqualysin I (AQN) variant D138N, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant D17N | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 110 | 0.98 | 113 | pH 7.5, 40 °C | Open record: Aqualysin I (AQN) variant D17N, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant D183N | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 53 | 0.74 | 71.6 | pH 7.5, 40 °C | Open record: Aqualysin I (AQN) variant D183N, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant D212N | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 75.2 | 1.1 | 68.6 | pH 7.5, 40 °C | Open record: Aqualysin I (AQN) variant D212N, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant D58N | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 68.5 | 0.99 | 70 | pH 7.5, 40 °C | Open record: Aqualysin I (AQN) variant D58N, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant E237Q | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 96.1 | 0.91 | 105 | pH 7.5, 40 °C | Open record: Aqualysin I (AQN) variant E237Q, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant G262D | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 61.3 | 0.93 | 66.2 | pH 7.5, 40 °C | Open record: Aqualysin I (AQN) variant G262D, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant G61D | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 47.2 | 0.82 | 63.6 | pH 7.5, 40 °C | Open record: Aqualysin I (AQN) variant G61D, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Aqualysin I (AQN)variant S277D | Thermus aquaticus | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 73.6 | 0.8 | 92.5 | pH 7.5, 40 °C | Open record: Aqualysin I (AQN) variant S277D, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |