CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mutagenesis of histidinol dehydrogenase reveals roles for conserved histidine residues

PMID 10353848doi:10.1021/bi982758pOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (32 values) from this paper: L-histidinol dehydrogenase (HDH) from Salmonella typhimurium.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.23

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
L-histidinol dehydrogenase (HDH)Salmonella typhimuriumimidazolyl propanediol0.080.240.33 calc.pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)Salmonella typhimuriumL-histidinol130.015870 calc.pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)Salmonella typhimuriumNAD+NADH production at 340 nm (standard assay, Loper method)—1.1—pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)Salmonella typhimuriumNAD+NAD 0.2-10 mM with 2 mM imidazolyl propanediol—7.3—pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H261NSalmonella typhimuriumL-histidinol0.00190.03110.061 calc.pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H261NSalmonella typhimuriumNAD+—2.1—pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H326NSalmonella typhimuriumimidazolyl propanediol0.0030.0260.12 calc.pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H326NSalmonella typhimuriumL-histidinolNADH production at 340 nm (standard assay, Loper method)0.0250.001715 calc.pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H326NSalmonella typhimuriumL-histidinol0.012——pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H326NSalmonella typhimuriumNAD+NADH production at 340 nm (standard assay, Loper method)—0.9—pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H326NSalmonella typhimuriumNAD+NAD 0.2-10 mM with 2 mM imidazolyl propanediol—0.7—pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H366NSalmonella typhimuriumimidazolyl propanediol0.00270.90.0030 calc.pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H366NSalmonella typhimuriumL-histidinol4.90.111944 calc.pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H366NSalmonella typhimuriumNAD+NADH production at 340 nm (standard assay, Loper method)—1.3—pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H366NSalmonella typhimuriumNAD+NAD 0.2-10 mM with 10 mM imidazolyl propanediol—0.9—pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H418QSalmonella typhimuriumimidazolyl propanediol0.0010.450.0022 calc.pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H418QSalmonella typhimuriumL-histidinol0.580.016535 calc.pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H418QSalmonella typhimuriumNAD+NADH production at 340 nm (standard assay, Loper method)—15.3—pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H418QSalmonella typhimuriumNAD+NAD 0.2-10 mM with 5.8 mM imidazolyl propanediol—14—pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H98NSalmonella typhimuriumimidazolyl propanediol0.04——pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H98NSalmonella typhimuriumL-histidinol3.30.0156210 calc.pH 9, 30 °COpen
L-histidinol dehydrogenase (HDH)variant H98NSalmonella typhimuriumNAD+—0.6—pH 9, 30 °COpen