Values CatLog read from this paper

Substrate recognition and selectivity of peptide deformylase. Similarities and differences with metzincins and thermolysin

PMID 10373378doi:10.1006/jmbi.1999.2832Open in Browse

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CatLog read 69 records (159 values) from this paper: peptide deformylase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.5.1.88

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
peptide deformylase	Escherichia coli	Fo-Ala-Yaa-NH2 sub-library (P1' = Ala)	—	—	0.84	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Ala-Yaa-NH2 sub-library (P1' = Ala)
peptide deformylase	Escherichia coli	Fo-Arg-Yaa-NH2 sub-library (P1' = Arg)	—	—	0.015	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Arg-Yaa-NH2 sub-library (P1' = Arg)
peptide deformylase	Escherichia coli	Fo-Asn-Yaa-NH2 sub-library (P1' = Asn)	—	—	0.011	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Asn-Yaa-NH2 sub-library (P1' = Asn)
peptide deformylase	Escherichia coli	Fo-Asp-Yaa-NH2 sub-library (P1' = Asp)	—	—	9 × 10⁻⁴	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Asp-Yaa-NH2 sub-library (P1' = Asp)
peptide deformylase	Escherichia coli	Fo-Cys(Bzl)-Yaa-NH2 sub-library (P1' = CysBzl)	—	—	6.4	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Cys(Bzl)-Yaa-NH2 sub-library (P1' = CysBzl)
peptide deformylase	Escherichia coli	Fo-Cys(OMeBzl)-Yaa-NH2 sub-library (P1' = Cys(OMeBzl))	—	—	6.5	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Cys(OMeBzl)-Yaa-NH2 sub-library (P1' = Cys(OMeBzl))
peptide deformylase	Escherichia coli	Fo-Gln-Yaa-NH2 sub-library (P1' = Gln)	—	—	1.8	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Gln-Yaa-NH2 sub-library (P1' = Gln)
peptide deformylase	Escherichia coli	Fo-Glu-Yaa-NH2 sub-library (P1' = Glu)	—	—	0.018	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Glu-Yaa-NH2 sub-library (P1' = Glu)
peptide deformylase	Escherichia coli	Fo-Gly-Yaa-NH2 sub-library (P1' = Gly)	—	—	0.017	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Gly-Yaa-NH2 sub-library (P1' = Gly)
peptide deformylase	Escherichia coli	Fo-His-Yaa-NH2 sub-library (P1' = His)	—	—	0.07	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-His-Yaa-NH2 sub-library (P1' = His)
peptide deformylase	Escherichia coli	Fo-homo-Phe-Yaa-NH2 sub-library (P1' = h Phe)	—	—	3.3	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-homo-Phe-Yaa-NH2 sub-library (P1' = h Phe)
peptide deformylase	Escherichia coli	Fo-Ile-Yaa-NH2 sub-library (P1' = Ile)	—	—	1.8	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Ile-Yaa-NH2 sub-library (P1' = Ile)
peptide deformylase	Escherichia coli	Fo-Leu-Yaa-NH2 sub-library (P1' = Leu)	—	—	3	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Leu-Yaa-NH2 sub-library (P1' = Leu)
peptide deformylase	Escherichia coli	Fo-Lys-Yaa-NH2 sub-library (P1' = Lys)	—	—	0.05	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Lys-Yaa-NH2 sub-library (P1' = Lys)
peptide deformylase	Escherichia coli	Fo-Met-Yaa-NH2 sub-library (P1' = Met)	270	2.1	131	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Met-Yaa-NH2 sub-library (P1' = Met)
peptide deformylase	Escherichia coli	Fo-n-hexyl side chain-Yaa-NH2 sub-library (P1' = n Hex)	130	2.3	57	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-n-hexyl side chain-Yaa-NH2 sub-library (P1' = n Hex)
peptide deformylase	Escherichia coli	Fo-n-pentyl side chain-Yaa-NH2 sub-library (P1' = n Pent)	230	3.2	72	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-n-pentyl side chain-Yaa-NH2 sub-library (P1' = n Pent)
peptide deformylase	Escherichia coli	Fo-Nle (n-butyl)-Yaa-NH2 sub-library (P1' = Nle (n But))	220	1.1	166	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Nle (n-butyl)-Yaa-NH2 sub-library (P1' = Nle (n But))
peptide deformylase	Escherichia coli	Fo-p-Phe-Yaa-NH2 sub-library (P1' = p Phe)	—	—	10	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-p-Phe-Yaa-NH2 sub-library (P1' = p Phe)
peptide deformylase	Escherichia coli	Fo-Phe-Yaa-NH2 sub-library (P1' = Phe)	100	2.7	37	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Phe-Yaa-NH2 sub-library (P1' = Phe)
peptide deformylase	Escherichia coli	Fo-Ser(Bzl)-Yaa-NH2 sub-library (P1' = Ser(Bzl))	—	—	14	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Ser(Bzl)-Yaa-NH2 sub-library (P1' = Ser(Bzl))
peptide deformylase	Escherichia coli	Fo-Ser-Yaa-NH2 sub-library (P1' = Ser)	—	—	0.04	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Ser-Yaa-NH2 sub-library (P1' = Ser)
peptide deformylase	Escherichia coli	Fo-Thr-Yaa-NH2 sub-library (P1' = Thr)	—	—	0.034	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Thr-Yaa-NH2 sub-library (P1' = Thr)
peptide deformylase	Escherichia coli	Fo-Tyr-Yaa-NH2 sub-library (P1' = Tyr)	—	—	2.4	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Tyr-Yaa-NH2 sub-library (P1' = Tyr)
peptide deformylase	Escherichia coli	Fo-Val-Yaa-NH2 sub-library (P1' = Val)	—	—	0.19	pH 7.5, 37 °C	Open record: peptide deformylase, Fo-Val-Yaa-NH2 sub-library (P1' = Val)
peptide deformylase	Escherichia coli	N-formyl-norleucyl-alaninamide	270	1.3	210	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-alaninamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-alpha-aspartamide	120	4.3	29	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-alpha-aspartamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-alpha-glutamamide	200	4.3	46	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-alpha-glutamamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-argininamide	300	0.66	450	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-argininamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-asparaginamide	320	8.2	32	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-asparaginamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-glutaminamide	280	1.5	200	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-glutaminamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-glycinamide	260	5.5	47	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-glycinamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-histidinamide	250	1.8	140	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-histidinamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-leucinamide	150	1.8	81	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-leucinamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-lysinamide	230	0.62	360	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-lysinamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-methioninamide	190	1.7	110	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-methioninamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-norleucinamide	210	2.9	72	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-norleucinamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-phenylalaninamide	200	3	65	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-phenylalaninamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-prolinamide	150	3.7	41	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-prolinamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-serinamide	170	1	170	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-serinamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-threoninamide	180	1.6	110	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-threoninamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-tryptophanamide	130	1.9	69	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-tryptophanamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-tyrosinamide	140	3.3	42	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-tyrosinamide
peptide deformylase	Escherichia coli	N-formyl-norleucyl-valinamide	160	1.9	85	pH 7.5, 37 °C	Open record: peptide deformylase, N-formyl-norleucyl-valinamide
peptide deformylasevariant A47S	Escherichia coli	N-formyl-methionyl-alanine	53	5.9	9	pH 7.5, 37 °C	Open record: peptide deformylase variant A47S, N-formyl-methionyl-alanine
peptide deformylasevariant C129A	Escherichia coli	N-formyl-methionyl-alanine	200	8.2	24	pH 7.5, 37 °C	Open record: peptide deformylase variant C129A, N-formyl-methionyl-alanine
peptide deformylasevariant C129W	Escherichia coli	N-formyl-methionyl-alanine	—	—	0.015	pH 7.5, 37 °C	Open record: peptide deformylase variant C129W, N-formyl-methionyl-alanine
peptide deformylasevariant E133Q	Escherichia coli	N-formyl-methionyl-alanine	5.1	0.011	0.0022	pH 7.5, 37 °C	Open record: peptide deformylase variant E133Q, N-formyl-methionyl-alanine
peptide deformylasevariant E88A	Escherichia coli	N-formyl-methionyl-alanine	0.83	6.7	0.12	pH 7.5, 37 °C	Open record: peptide deformylase variant E88A, N-formyl-methionyl-alanine
peptide deformylasevariant G45A	Escherichia coli	N-formyl-methionyl-alanine	129	13.8	9.3	pH 7.5, 37 °C	Open record: peptide deformylase variant G45A, N-formyl-methionyl-alanine
peptide deformylasevariant G89P	Escherichia coli	N-formyl-methionyl-alanine	—	—	0.012	pH 7.5, 37 °C	Open record: peptide deformylase variant G89P, N-formyl-methionyl-alanine
peptide deformylasevariant G89S	Escherichia coli	N-formyl-methionyl-alanine	11.4	13	0.9	pH 7.5, 37 °C	Open record: peptide deformylase variant G89S, N-formyl-methionyl-alanine
peptide deformylasevariant I128A	Escherichia coli	N-formyl-methionyl-alanine	—	—	3.9	pH 7.5, 37 °C	Open record: peptide deformylase variant I128A, N-formyl-methionyl-alanine
peptide deformylasevariant I128F	Escherichia coli	N-formyl-methionyl-alanine	0.3	2.3	0.1	pH 7.5, 37 °C	Open record: peptide deformylase variant I128F, N-formyl-methionyl-alanine
peptide deformylasevariant I128R	Escherichia coli	N-formyl-methionyl-alanine	1.5	40	0.4	pH 7.5, 37 °C	Open record: peptide deformylase variant I128R, N-formyl-methionyl-alanine
peptide deformylasevariant I128S	Escherichia coli	N-formyl-methionyl-alanine	25.8	7.8	3.2	pH 7.5, 37 °C	Open record: peptide deformylase variant I128S, N-formyl-methionyl-alanine
peptide deformylasevariant I130F	Escherichia coli	N-formyl-methionyl-alanine	135	4.8	28	pH 7.5, 37 °C	Open record: peptide deformylase variant I130F, N-formyl-methionyl-alanine
peptide deformylasevariant I44F	Escherichia coli	N-formyl-methionyl-alanine	9	2.1	4.4	pH 7.5, 37 °C	Open record: peptide deformylase variant I44F, N-formyl-methionyl-alanine
peptide deformylasevariant I86A	Escherichia coli	N-formyl-methionyl-alanine	20.7	9	2.3	pH 7.5, 37 °C	Open record: peptide deformylase variant I86A, N-formyl-methionyl-alanine
peptide deformylasevariant I86F	Escherichia coli	N-formyl-methionyl-alanine	7.7	4	1.9	pH 7.5, 37 °C	Open record: peptide deformylase variant I86F, N-formyl-methionyl-alanine
peptide deformylasevariant L125A	Escherichia coli	N-formyl-methionyl-alanine	6.7	18.7	0.36	pH 7.5, 37 °C	Open record: peptide deformylase variant L125A, N-formyl-methionyl-alanine
peptide deformylasevariant L125W	Escherichia coli	N-formyl-methionyl-alanine	2.1	11.6	0.18	pH 7.5, 37 °C	Open record: peptide deformylase variant L125W, N-formyl-methionyl-alanine
peptide deformylasevariant L126F	Escherichia coli	N-formyl-methionyl-alanine	264	9.1	29	pH 7.5, 37 °C	Open record: peptide deformylase variant L126F, N-formyl-methionyl-alanine
peptide deformylasevariant L91A	Escherichia coli	N-formyl-methionyl-alanine	—	—	0.3	pH 7.5, 37 °C	Open record: peptide deformylase variant L91A, N-formyl-methionyl-alanine
peptide deformylasevariant Q50A	Escherichia coli	N-formyl-methionyl-alanine	0.061	8	0.008	pH 7.5, 37 °C	Open record: peptide deformylase variant Q50A, N-formyl-methionyl-alanine
peptide deformylasevariant Q50E	Escherichia coli	N-formyl-methionyl-alanine	0.033	2.3	0.014	pH 7.5, 37 °C	Open record: peptide deformylase variant Q50E, N-formyl-methionyl-alanine
peptide deformylasevariant Q50K	Escherichia coli	N-formyl-methionyl-alanine	0.0019	6.7	2.8 × 10⁻⁴	pH 7.5, 37 °C	Open record: peptide deformylase variant Q50K, N-formyl-methionyl-alanine
peptide deformylasevariant Q50M	Escherichia coli	N-formyl-methionyl-alanine	0.28	10.1	0.028	pH 7.5, 37 °C	Open record: peptide deformylase variant Q50M, N-formyl-methionyl-alanine
peptide deformylasevariant Q50N	Escherichia coli	N-formyl-methionyl-alanine	0.022	6.5	0.0035	pH 7.5, 37 °C	Open record: peptide deformylase variant Q50N, N-formyl-methionyl-alanine