CatLogenzyme kinetics catalog

Values CatLog read from this paper

Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum

PMID 10447682doi:10.1046/j.1432-1327.1999.00586.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 49 records (84 values) from this paper: cholesterol oxidase (SCO), cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) from Streptomyces hygroscopicus, Brevibacterium sterolicum.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 35 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.3.6

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicum5-androstene-3β-ol-17-one0.60.61.0 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicum5-androstene-3β-ol-17-onetest 4: H2O2 production at 440 nm0.22.60.077 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicum5-cholestene-3β-ol500 mM potassium phosphate; 0.4% Thesit1060.2530 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicum5-cholestene-3β-oltest 4: H2O2 production at 440 nm; 0.4% Thesit261.616 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicum5-cholestene-3β-oltest 1: polarographic O2 consumption420.11380 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicum5-pregnene-3β-ol-20-one440.949 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicum5-pregnene-3β-ol-20-onetest 4: H2O2 production at 440 nm122.15.7 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicum5α-androstan-3β-ol-17-one0.11.50.067 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicum5α-cholestan-3β-oltest 4: H2O2 production at 440 nm; 0.4% Thesit120.620 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicum5α-cholestan-3β-oltest 5: H2O2 production at 440 nm; 0.2% Thesit—0.02—pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumandrost-5-en-3β-ol-β-17-sulfate0.051.40.036 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumandrost-5-en-3β-ol-β-17-sulfatetest 4: H2O2 production at 440 nm0.023.40.0059 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumcholest-5-en-3-one2500.3830 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumcholesterolassay A: H2O2 formation; 50 mM potassium phosphate; Table 4480.11440 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumcholesterolassay B: enzyme-monitored turnover (EMTN, stopped-flow)19.20.2191 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumcholesterolassay B: enzyme-monitored turnover (EMTN, stopped-flow)104.50.87120 calc.pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumcholesterol500 mM potassium phosphate; 1% Thesit; 1.25% 2-propanol51——pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumcholesterol500 mM potassium phosphate; 1% Thesit; 10% 2-propanol50.8——pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumO2assay A: H2O2 formation; no Thesit; no 2-propanol—0.165—pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumO2assay B: enzyme-monitored turnover (EMTN, stopped-flow)—0.135—pH 7.5, 25 °COpen
cholesterol oxidase (BCO, covalent 8-histidyl-FAD form)Brevibacterium sterolicumO2assay B: enzyme-monitored turnover (EMTN, stopped-flow)—0.107—pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicus5-androstene-3β-ol-17-one220.1220 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicus5-androstene-3β-ol-17-onetest 4: H2O2 production at 440 nm0.90.19.0 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicus5-cholestene-3β-ol500 mM potassium phosphate; 0.4% Thesit2712.2120 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicus5-cholestene-3β-oltest 1: polarographic O2 consumption10.60.2641 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicus5-cholestene-3β-oltest 4: H2O2 production at 440 nm; 0.4% Thesit20.45.0 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicus5-cholestene-3β-oltest 5: H2O2 production at 440 nm; 0.2% Thesit31——pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicus5-pregnene-3β-ol-20-one820.3270 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicus5-pregnene-3β-ol-20-onetest 4: H2O2 production at 440 nm30.0838 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicus5α-androstan-3β-ol-17-one0.70.23.5 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicus5α-cholestan-3β-ol60.87.5 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicusandrost-5-en-3β-ol-β-17-sulfate0.60.23.0 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicusandrost-5-en-3β-ol-β-17-sulfatetest 4: H2O2 production at 440 nm0.21.50.13 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicuscholest-5-en-3-one50 mM phosphate buffer6703.9170 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicuscholest-5-en-3-one500 mM phosphate buffer3301.5220 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicuscholesterolassay A: H2O2 formation; 100 mM potassium phosphate280.2140 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicuscholesterolassay A: H2O2 formation; 50 mM potassium phosphate1400.57250 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicuscholesterolassay A: H2O2 formation; 50 mM potassium phosphate3452.41143 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicuscholesterolassay A: H2O2 formation; 50 mM potassium phosphate15.81.1314.0 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicuscholesterolassay B: enzyme-monitored turnover (EMTN, stopped-flow)67.60.041.7 × 10³ calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicuscholesterolassay B: enzyme-monitored turnover (EMTN, stopped-flow)202.20.91220 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicuscholesterolassay B: enzyme-monitored turnover (EMTN, stopped-flow)3.20.0480 calc.pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicusO2assay A: H2O2 formation; 100 mM potassium phosphate—0.53—pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicusO2assay A: H2O2 formation; 50 mM potassium phosphate—0.2—pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicusO2assay A: H2O2 formation; 50 mM potassium phosphate—0.76—pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicusO2assay A: H2O2 formation; 50 mM potassium phosphate—0.61—pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicusO2assay B: enzyme-monitored turnover (EMTN, stopped-flow)—1.75—pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicusO2assay B: enzyme-monitored turnover (EMTN, stopped-flow)—0.92—pH 7.5, 25 °COpen
cholesterol oxidase (SCO)Streptomyces hygroscopicusO2assay B: enzyme-monitored turnover (EMTN, stopped-flow)—0.15—pH 7.5, 25 °COpen