EC 1.1.3.6: cholesterol oxidase
OxidoreductasesOpen in Browse
CatLog read 107 records for EC 1.1.3.6 from 4 papers, for 5 organisms. Names in these papers: cholesterol oxidase (SCO), cholesterol oxidase (ChoAS), cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), cholesterol oxidase (ChoA) and 1 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 95 records for EC 1.1.3.6 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.1.3.6 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum | Brevibacterium sterolicum, Streptomyces hygroscopicus | 49 |
| Alteration of substrate specificity of cholesterol oxidase from Streptomyces sp. by site-directed mutagenesis | Streptomyces sp. | 22 |
| Rational design of cholesterol oxidase for efficient bioresolution of cholestane skeleton substrates | Pimelobacter simplex | 18 |
| Use of an Isotope-Coded Mass Tag (ICMT) Method To Determine the Orientation of Cholesterol Oxidase on Model Membranes | Streptomyces | 18 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | 5-androstene-3β-ol-17-one | 0.6 | 0.6 | 1.0 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), 5-androstene-3β-ol-17-one |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | 5-androstene-3β-ol-17-onetest 4: H2O2 production at 440 nm | 0.2 | 2.6 | 0.077 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), 5-androstene-3β-ol-17-one |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | 5-cholestene-3β-ol500 mM potassium phosphate; 0.4% Thesit | 106 | 0.2 | 530 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), 5-cholestene-3β-ol |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | 5-cholestene-3β-oltest 4: H2O2 production at 440 nm; 0.4% Thesit | 26 | 1.6 | 16 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), 5-cholestene-3β-ol |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | 5-cholestene-3β-oltest 1: polarographic O2 consumption | 42 | 0.11 | 380 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), 5-cholestene-3β-ol |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | 5-pregnene-3β-ol-20-one | 44 | 0.9 | 49 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), 5-pregnene-3β-ol-20-one |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | 5-pregnene-3β-ol-20-onetest 4: H2O2 production at 440 nm | 12 | 2.1 | 5.7 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), 5-pregnene-3β-ol-20-one |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | 5α-androstan-3β-ol-17-one | 0.1 | 1.5 | 0.067 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), 5α-androstan-3β-ol-17-one |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | 5α-cholestan-3β-oltest 4: H2O2 production at 440 nm; 0.4% Thesit | 12 | 0.6 | 20 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), 5α-cholestan-3β-ol |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | 5α-cholestan-3β-oltest 5: H2O2 production at 440 nm; 0.2% Thesit | — | 0.02 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), 5α-cholestan-3β-ol |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | androst-5-en-3β-ol-β-17-sulfate | 0.05 | 1.4 | 0.036 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), androst-5-en-3β-ol-β-17-sulfate |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | androst-5-en-3β-ol-β-17-sulfatetest 4: H2O2 production at 440 nm | 0.02 | 3.4 | 0.0059 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), androst-5-en-3β-ol-β-17-sulfate |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | cholest-5-en-3-one | 250 | 0.3 | 830 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), cholest-5-en-3-one |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | cholesterolassay A: H2O2 formation; 50 mM potassium phosphate; Table 4 | 48 | 0.11 | 440 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), cholesterol |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | cholesterolassay B: enzyme-monitored turnover (EMTN, stopped-flow) | 19.2 | 0.21 | 91 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), cholesterol |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | cholesterolassay B: enzyme-monitored turnover (EMTN, stopped-flow) | 104.5 | 0.87 | 120 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), cholesterol |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | cholesterol500 mM potassium phosphate; 1% Thesit; 1.25% 2-propanol | 51 | — | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), cholesterol |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | cholesterol500 mM potassium phosphate; 1% Thesit; 10% 2-propanol | 50.8 | — | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), cholesterol |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | O2assay A: H2O2 formation; no Thesit; no 2-propanol | — | 0.165 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), O2 |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | O2assay B: enzyme-monitored turnover (EMTN, stopped-flow) | — | 0.135 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), O2 |
| cholesterol oxidase (BCO, covalent 8-histidyl-FAD form) | Brevibacterium sterolicum | O2assay B: enzyme-monitored turnover (EMTN, stopped-flow) | — | 0.107 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (BCO, covalent 8-histidyl-FAD form), O2 |
| cholesterol oxidase (ChoA) | Streptomyces | cholesterolTable 1 | 67 | 0.013 | 5.1 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA), cholesterol |
| cholesterol oxidase (ChoA) | Streptomyces | cholesterolTable 2 | 20 | 0.011 | 1.8 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA), cholesterol |
| cholesterol oxidase (ChoA)variant A184C/A301C/T394C (mutant 8) | Streptomyces | cholesterol | 57 | 0.011 | 5 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant A184C/A301C/T394C (mutant 8), cholesterol |
| cholesterol oxidase (ChoA)variant A184C/T239C/A407C/A465C (mutant 6) | Streptomyces | cholesterol | 60 | 0.013 | 4.6 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant A184C/T239C/A407C/A465C (mutant 6), cholesterol |
| cholesterol oxidase (ChoA)variant A32C/S129C/T371C/A423C (mutant 3) | Streptomyces | cholesterol | 23 | 0.011 | 2 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant A32C/S129C/T371C/A423C (mutant 3), cholesterol |
| cholesterol oxidase (ChoA)variant A32C/T168C/S312C/A465C (mutant 7) | Streptomyces | cholesterol | 30 | 0.012 | 2.4 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant A32C/T168C/S312C/A465C (mutant 7), cholesterol |
| cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/A205C (single-cysteine A205C) | Streptomyces | cholesterol | 14 | 0.008 | 1.1 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant C56A/C282A/C445A/C452A/A205C (single-cysteine A205C), cholesterol |
| cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/A367C (single-cysteine A367C) | Streptomyces | cholesterol | 29 | 0.017 | 1.7 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant C56A/C282A/C445A/C452A/A367C (single-cysteine A367C), cholesterol |
| cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/L369C (single-cysteine L369C) | Streptomyces | cholesterol | 21 | 0.014 | 1.4 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant C56A/C282A/C445A/C452A/L369C (single-cysteine L369C), cholesterol |
| cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/L80C (single-cysteine L80C) | Streptomyces | cholesterol | 22 | 0.012 | 1.8 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant C56A/C282A/C445A/C452A/L80C (single-cysteine L80C), cholesterol |
| cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/T168C (single-cysteine T168C) | Streptomyces | cholesterol | 23 | 0.014 | 1.6 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant C56A/C282A/C445A/C452A/T168C (single-cysteine T168C), cholesterol |
| cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/V176C (single-cysteine V176C) | Streptomyces | cholesterol | 38 | 0.019 | 2 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant C56A/C282A/C445A/C452A/V176C (single-cysteine V176C), cholesterol |
| cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/W81C (single-cysteine W81C) | Streptomyces | cholesterol | 21 | 0.013 | 1.6 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant C56A/C282A/C445A/C452A/W81C (single-cysteine W81C), cholesterol |
| cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/W81C/A367C/T168C (WCAC, W81C-A367C/T168C) | Streptomyces | cholesterol | 40 | 0.019 | 2.1 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant C56A/C282A/C445A/C452A/W81C/A367C/T168C (WCAC, W81C-A367C/T168C), cholesterol |
| cholesterol oxidase (ChoA)variant L274C (mutant 2) | Streptomyces | cholesterol | 75 | 0.017 | 4.5 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant L274C (mutant 2), cholesterol |
| cholesterol oxidase (ChoA)variant L80C (mutant 1) | Streptomyces | cholesterol | 89 | 0.011 | 8.4 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant L80C (mutant 1), cholesterol |
| cholesterol oxidase (ChoA)variant S153C/A205C/S312C/T435C (mutant 4) | Streptomyces | cholesterol | 68 | 0.01 | 7 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant S153C/A205C/S312C/T435C (mutant 4), cholesterol |
| cholesterol oxidase (ChoA)variant T168C/A276C (mutant 5) | Streptomyces | cholesterol | 46 | 0.014 | 3.4 × 10³ | pH 7, 37 °C | PMID 30125103 | Open record: cholesterol oxidase (ChoA) variant T168C/A276C (mutant 5), cholesterol |
| cholesterol oxidase (ChoAS) | Streptomyces sp. | 5-cholesten-3β-ol | 40 | 2 × 10⁻⁵ | 2 × 10⁶ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS), 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS) | Streptomyces sp. | pregnenolone | 30 | 2.3 × 10⁻⁵ | 1.3 × 10⁶ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS), pregnenolone |
| cholesterol oxidase (ChoAS)variant L119A | Streptomyces sp. | 5-cholesten-3β-ol | 15 | 8.1 × 10⁻⁵ | 1.9 × 10⁵ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant L119A, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant L119A | Streptomyces sp. | pregnenolone | 13 | 4 × 10⁻⁵ | 3.3 × 10⁵ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant L119A, pregnenolone |
| cholesterol oxidase (ChoAS)variant L119F | Streptomyces sp. | 5-cholesten-3β-ol | 27 | 1.7 × 10⁻⁴ | 1.6 × 10⁵ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant L119F, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant L119F | Streptomyces sp. | pregnenolone | 21 | 7.7 × 10⁻⁵ | 2.7 × 10⁵ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant L119F, pregnenolone |
| cholesterol oxidase (ChoAS)variant P357N | Streptomyces sp. | 5-cholesten-3β-ol | 9 | 1.1 × 10⁻⁴ | 8.2 × 10⁴ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant P357N, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant P357N | Streptomyces sp. | pregnenolone | 9 | 9.8 × 10⁻⁵ | 9.2 × 10⁴ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant P357N, pregnenolone |
| cholesterol oxidase (ChoAS)variant Q286R | Streptomyces sp. | 5-cholesten-3β-ol | 23 | 1.3 × 10⁻⁴ | 1.8 × 10⁵ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant Q286R, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant Q286R | Streptomyces sp. | pregnenolone | 19 | 5.6 × 10⁻⁵ | 3.4 × 10⁵ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant Q286R, pregnenolone |
| cholesterol oxidase (ChoAS)variant S379A | Streptomyces sp. | 5-cholesten-3β-ol | 49 | 3 × 10⁻⁵ | 1.6 × 10⁶ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant S379A, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant S379A | Streptomyces sp. | pregnenolone | 35 | 2.3 × 10⁻⁵ | 1.5 × 10⁶ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant S379A, pregnenolone |
| cholesterol oxidase (ChoAS)variant S379T | Streptomyces sp. | 5-cholesten-3β-ol | 29 | 8.1 × 10⁻⁶ | 3.6 × 10⁶ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant S379T, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant S379T | Streptomyces sp. | pregnenolone | 45 | 5.8 × 10⁻⁶ | 7.8 × 10⁶ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant S379T, pregnenolone |
| cholesterol oxidase (ChoAS)variant S379V | Streptomyces sp. | 5-cholesten-3β-ol | 51 | 4.6 × 10⁻⁵ | 1.1 × 10⁶ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant S379V, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant S379V | Streptomyces sp. | pregnenolone | 39 | 4 × 10⁻⁵ | 9.8 × 10⁵ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant S379V, pregnenolone |
| cholesterol oxidase (ChoAS)variant V145D | Streptomyces sp. | 5-cholesten-3β-ol | 11 | 4 × 10⁻⁴ | 2.8 × 10⁴ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant V145D, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant V145D | Streptomyces sp. | pregnenolone | 9 | 1 × 10⁻⁴ | 9 × 10⁴ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant V145D, pregnenolone |
| cholesterol oxidase (ChoAS)variant V145E | Streptomyces sp. | 5-cholesten-3β-ol | 23 | 6.7 × 10⁻⁴ | 3.4 × 10⁴ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant V145E, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant V145E | Streptomyces sp. | pregnenolone | 11 | 8.3 × 10⁻⁵ | 1.3 × 10⁵ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant V145E, pregnenolone |
| cholesterol oxidase (ChoAS)variant V145Q | Streptomyces sp. | 5-cholesten-3β-ol | 11 | 7.2 × 10⁻⁵ | 1.5 × 10⁵ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant V145Q, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant V145Q | Streptomyces sp. | pregnenolone | 12 | 5.8 × 10⁻⁵ | 2.1 × 10⁵ | pH 7, 37 °C | PMID 12082166 | Open record: cholesterol oxidase (ChoAS) variant V145Q, pregnenolone |
| cholesterol oxidase (PsChO) | Pimelobacter simplex | cholesterol | 12.29 | 0.2049 | 60 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO), cholesterol |
| cholesterol oxidase (PsChO) | Pimelobacter simplex | dehydroepiandrosterone | 6.82 | 0.3412 | 20 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO), dehydroepiandrosterone |
| cholesterol oxidase (PsChO) | Pimelobacter simplex | pregnenolone | 11.78 | 0.2945 | 40 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO), pregnenolone |
| cholesterol oxidase (PsChO) | Pimelobacter simplex | stigmasterol | 1.58 | 0.7896 | 2 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO), stigmasterol |
| cholesterol oxidase (PsChO) | Pimelobacter simplex | β-sitosterol | 9.86 | 0.3286 | 30 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO), β-sitosterol |
| cholesterol oxidase (PsChO)variant F70A | Pimelobacter simplex | cholesterol | — | — | 80 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant F70A, cholesterol |
| cholesterol oxidase (PsChO)variant F70V | Pimelobacter simplex | cholesterol | 15.48 | 0.1548 | 100 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant F70V, cholesterol |
| cholesterol oxidase (PsChO)variant F70V | Pimelobacter simplex | dehydroepiandrosterone | 13.68 | 0.2735 | 50 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant F70V, dehydroepiandrosterone |
| cholesterol oxidase (PsChO)variant F70V | Pimelobacter simplex | pregnenolone | 15.25 | 0.1906 | 80 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant F70V, pregnenolone |
| cholesterol oxidase (PsChO)variant F70V | Pimelobacter simplex | stigmasterol | 7.68 | 0.3841 | 20 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant F70V, stigmasterol |
| cholesterol oxidase (PsChO)variant F70V | Pimelobacter simplex | β-sitosterol | 16.06 | 0.1784 | 90 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant F70V, β-sitosterol |
| cholesterol oxidase (PsChO)variant V64C | Pimelobacter simplex | cholesterol | 23.02 | 0.1783 | 130 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant V64C, cholesterol |
| cholesterol oxidase (PsChO)variant V64C | Pimelobacter simplex | dehydroepiandrosterone | 9.56 | 0.3188 | 30 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant V64C, dehydroepiandrosterone |
| cholesterol oxidase (PsChO)variant V64C | Pimelobacter simplex | pregnenolone | 13.71 | 0.2284 | 60 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant V64C, pregnenolone |
| cholesterol oxidase (PsChO)variant V64C | Pimelobacter simplex | stigmasterol | 4.23 | 0.5288 | 8 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant V64C, stigmasterol |
| cholesterol oxidase (PsChO)variant V64C | Pimelobacter simplex | β-sitosterol | 13.25 | 0.2208 | 60 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant V64C, β-sitosterol |
| cholesterol oxidase (PsChO)variant V64I | Pimelobacter simplex | cholesterol | — | — | 100 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant V64I, cholesterol |
| cholesterol oxidase (PsChO)variant V64L | Pimelobacter simplex | cholesterol | — | — | 100 | pH 7.5, 25 °C | PMID 29180806 | Open record: cholesterol oxidase (PsChO) variant V64L, cholesterol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | 5-androstene-3β-ol-17-one | 22 | 0.1 | 220 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), 5-androstene-3β-ol-17-one |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | 5-androstene-3β-ol-17-onetest 4: H2O2 production at 440 nm | 0.9 | 0.1 | 9.0 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), 5-androstene-3β-ol-17-one |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | 5-cholestene-3β-ol500 mM potassium phosphate; 0.4% Thesit | 271 | 2.2 | 120 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), 5-cholestene-3β-ol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | 5-cholestene-3β-oltest 1: polarographic O2 consumption | 10.6 | 0.26 | 41 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), 5-cholestene-3β-ol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | 5-cholestene-3β-oltest 4: H2O2 production at 440 nm; 0.4% Thesit | 2 | 0.4 | 5.0 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), 5-cholestene-3β-ol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | 5-cholestene-3β-oltest 5: H2O2 production at 440 nm; 0.2% Thesit | 31 | — | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), 5-cholestene-3β-ol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | 5-pregnene-3β-ol-20-one | 82 | 0.3 | 270 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), 5-pregnene-3β-ol-20-one |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | 5-pregnene-3β-ol-20-onetest 4: H2O2 production at 440 nm | 3 | 0.08 | 38 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), 5-pregnene-3β-ol-20-one |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | 5α-androstan-3β-ol-17-one | 0.7 | 0.2 | 3.5 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), 5α-androstan-3β-ol-17-one |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | 5α-cholestan-3β-ol | 6 | 0.8 | 7.5 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), 5α-cholestan-3β-ol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | androst-5-en-3β-ol-β-17-sulfate | 0.6 | 0.2 | 3.0 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), androst-5-en-3β-ol-β-17-sulfate |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | androst-5-en-3β-ol-β-17-sulfatetest 4: H2O2 production at 440 nm | 0.2 | 1.5 | 0.13 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), androst-5-en-3β-ol-β-17-sulfate |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | cholest-5-en-3-one50 mM phosphate buffer | 670 | 3.9 | 170 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), cholest-5-en-3-one |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | cholest-5-en-3-one500 mM phosphate buffer | 330 | 1.5 | 220 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), cholest-5-en-3-one |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | cholesterolassay A: H2O2 formation; 100 mM potassium phosphate | 28 | 0.2 | 140 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), cholesterol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | cholesterolassay A: H2O2 formation; 50 mM potassium phosphate | 140 | 0.57 | 250 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), cholesterol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | cholesterolassay A: H2O2 formation; 50 mM potassium phosphate | 345 | 2.41 | 143 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), cholesterol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | cholesterolassay A: H2O2 formation; 50 mM potassium phosphate | 15.8 | 1.13 | 14.0 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), cholesterol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | cholesterolassay B: enzyme-monitored turnover (EMTN, stopped-flow) | 67.6 | 0.04 | 1.7 × 10³ calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), cholesterol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | cholesterolassay B: enzyme-monitored turnover (EMTN, stopped-flow) | 202.2 | 0.91 | 220 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), cholesterol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | cholesterolassay B: enzyme-monitored turnover (EMTN, stopped-flow) | 3.2 | 0.04 | 80 calc. | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), cholesterol |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | O2assay A: H2O2 formation; 100 mM potassium phosphate | — | 0.53 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), O2 |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | O2assay A: H2O2 formation; 50 mM potassium phosphate | — | 0.2 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), O2 |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | O2assay A: H2O2 formation; 50 mM potassium phosphate | — | 0.76 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), O2 |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | O2assay A: H2O2 formation; 50 mM potassium phosphate | — | 0.61 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), O2 |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | O2assay B: enzyme-monitored turnover (EMTN, stopped-flow) | — | 1.75 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), O2 |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | O2assay B: enzyme-monitored turnover (EMTN, stopped-flow) | — | 0.92 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), O2 |
| cholesterol oxidase (SCO) | Streptomyces hygroscopicus | O2assay B: enzyme-monitored turnover (EMTN, stopped-flow) | — | 0.15 | — | pH 7.5, 25 °C | PMID 10447682 | Open record: cholesterol oxidase (SCO), O2 |