Values CatLog read from this paper
Alteration of substrate specificity of cholesterol oxidase from Streptomyces sp. by site-directed mutagenesis
PMID 12082166doi:10.1093/protein/15.6.477Open in Browse
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CatLog read 22 records (66 values) from this paper: cholesterol oxidase (ChoAS) from Streptomyces sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cholesterol oxidase (ChoAS) | Streptomyces sp. | 5-cholesten-3β-ol | 40 | 2 × 10⁻⁵ | 2 × 10⁶ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS), 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS) | Streptomyces sp. | pregnenolone | 30 | 2.3 × 10⁻⁵ | 1.3 × 10⁶ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS), pregnenolone |
| cholesterol oxidase (ChoAS)variant L119A | Streptomyces sp. | 5-cholesten-3β-ol | 15 | 8.1 × 10⁻⁵ | 1.9 × 10⁵ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant L119A, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant L119A | Streptomyces sp. | pregnenolone | 13 | 4 × 10⁻⁵ | 3.3 × 10⁵ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant L119A, pregnenolone |
| cholesterol oxidase (ChoAS)variant L119F | Streptomyces sp. | 5-cholesten-3β-ol | 27 | 1.7 × 10⁻⁴ | 1.6 × 10⁵ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant L119F, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant L119F | Streptomyces sp. | pregnenolone | 21 | 7.7 × 10⁻⁵ | 2.7 × 10⁵ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant L119F, pregnenolone |
| cholesterol oxidase (ChoAS)variant P357N | Streptomyces sp. | 5-cholesten-3β-ol | 9 | 1.1 × 10⁻⁴ | 8.2 × 10⁴ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant P357N, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant P357N | Streptomyces sp. | pregnenolone | 9 | 9.8 × 10⁻⁵ | 9.2 × 10⁴ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant P357N, pregnenolone |
| cholesterol oxidase (ChoAS)variant Q286R | Streptomyces sp. | 5-cholesten-3β-ol | 23 | 1.3 × 10⁻⁴ | 1.8 × 10⁵ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant Q286R, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant Q286R | Streptomyces sp. | pregnenolone | 19 | 5.6 × 10⁻⁵ | 3.4 × 10⁵ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant Q286R, pregnenolone |
| cholesterol oxidase (ChoAS)variant S379A | Streptomyces sp. | 5-cholesten-3β-ol | 49 | 3 × 10⁻⁵ | 1.6 × 10⁶ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant S379A, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant S379A | Streptomyces sp. | pregnenolone | 35 | 2.3 × 10⁻⁵ | 1.5 × 10⁶ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant S379A, pregnenolone |
| cholesterol oxidase (ChoAS)variant S379T | Streptomyces sp. | 5-cholesten-3β-ol | 29 | 8.1 × 10⁻⁶ | 3.6 × 10⁶ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant S379T, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant S379T | Streptomyces sp. | pregnenolone | 45 | 5.8 × 10⁻⁶ | 7.8 × 10⁶ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant S379T, pregnenolone |
| cholesterol oxidase (ChoAS)variant S379V | Streptomyces sp. | 5-cholesten-3β-ol | 51 | 4.6 × 10⁻⁵ | 1.1 × 10⁶ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant S379V, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant S379V | Streptomyces sp. | pregnenolone | 39 | 4 × 10⁻⁵ | 9.8 × 10⁵ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant S379V, pregnenolone |
| cholesterol oxidase (ChoAS)variant V145D | Streptomyces sp. | 5-cholesten-3β-ol | 11 | 4 × 10⁻⁴ | 2.8 × 10⁴ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant V145D, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant V145D | Streptomyces sp. | pregnenolone | 9 | 1 × 10⁻⁴ | 9 × 10⁴ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant V145D, pregnenolone |
| cholesterol oxidase (ChoAS)variant V145E | Streptomyces sp. | 5-cholesten-3β-ol | 23 | 6.7 × 10⁻⁴ | 3.4 × 10⁴ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant V145E, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant V145E | Streptomyces sp. | pregnenolone | 11 | 8.3 × 10⁻⁵ | 1.3 × 10⁵ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant V145E, pregnenolone |
| cholesterol oxidase (ChoAS)variant V145Q | Streptomyces sp. | 5-cholesten-3β-ol | 11 | 7.2 × 10⁻⁵ | 1.5 × 10⁵ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant V145Q, 5-cholesten-3β-ol |
| cholesterol oxidase (ChoAS)variant V145Q | Streptomyces sp. | pregnenolone | 12 | 5.8 × 10⁻⁵ | 2.1 × 10⁵ | pH 7, 37 °C | Open record: cholesterol oxidase (ChoAS) variant V145Q, pregnenolone |