CatLogenzyme kinetics catalog

Values CatLog read from this paper

Use of an Isotope-Coded Mass Tag (ICMT) Method To Determine the Orientation of Cholesterol Oxidase on Model Membranes

PMID 30125103doi:10.1021/acs.biochem.8b00788Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (54 values) from this paper: cholesterol oxidase (ChoA) from Streptomyces.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.3.6

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
cholesterol oxidase (ChoA)StreptomycescholesterolTable 1670.0135.1 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)StreptomycescholesterolTable 2200.0111.8 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant A184C/A301C/T394C (mutant 8)Streptomycescholesterol570.0115 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant A184C/T239C/A407C/A465C (mutant 6)Streptomycescholesterol600.0134.6 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant A32C/S129C/T371C/A423C (mutant 3)Streptomycescholesterol230.0112 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant A32C/T168C/S312C/A465C (mutant 7)Streptomycescholesterol300.0122.4 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/A205C (single-cysteine A205C)Streptomycescholesterol140.0081.1 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/A367C (single-cysteine A367C)Streptomycescholesterol290.0171.7 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/L369C (single-cysteine L369C)Streptomycescholesterol210.0141.4 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/L80C (single-cysteine L80C)Streptomycescholesterol220.0121.8 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/T168C (single-cysteine T168C)Streptomycescholesterol230.0141.6 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/V176C (single-cysteine V176C)Streptomycescholesterol380.0192 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/W81C (single-cysteine W81C)Streptomycescholesterol210.0131.6 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant C56A/C282A/C445A/C452A/W81C/A367C/T168C (WCAC, W81C-A367C/T168C)Streptomycescholesterol400.0192.1 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant L274C (mutant 2)Streptomycescholesterol750.0174.5 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant L80C (mutant 1)Streptomycescholesterol890.0118.4 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant S153C/A205C/S312C/T435C (mutant 4)Streptomycescholesterol680.017 × 10³pH 7, 37 °COpen
cholesterol oxidase (ChoA)variant T168C/A276C (mutant 5)Streptomycescholesterol460.0143.4 × 10³pH 7, 37 °COpen