CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of three key active site residues in the C-terminal domain of human recombinant folylpoly-gamma-glutamate synthetase by site-directed mutagenesis

PMID 10480915doi:10.1074/jbc.274.38.27018Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (32 values) from this paper: folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.2.17

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)Homo sapiensadenosine 5'-triphosphate—0.046—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)Homo sapiensaminopterin0.450.00950 calc.pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)Homo sapiensglutamic acid—0.235—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant C346AHomo sapiensadenosine 5'-triphosphate—0.04—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant C346AHomo sapiensaminopterin0.530.01341 calc.pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant C346AHomo sapiensglutamic acid—0.52—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant D335AHomo sapiensadenosine 5'-triphosphate—0.267—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant D335AHomo sapiensaminopterin0.420.182.3 calc.pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant D335AHomo sapiensglutamic acid—91—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant D376AHomo sapiensadenosine 5'-triphosphate—0.028—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant D376AHomo sapiensaminopterin0.550.03118 calc.pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant D376AHomo sapiensglutamic acid—2.9—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant D378AHomo sapiensadenosine 5'-triphosphate—0.06—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant D378AHomo sapiensaminopterin0.630.01737 calc.pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant D378AHomo sapiensglutamic acid—0.83—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant H338AHomo sapiensadenosine 5'-triphosphate—0.016—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant H338AHomo sapiensaminopterin0.240.006537 calc.pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant H338AHomo sapiensglutamic acid—140—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant K384AHomo sapiensadenosine 5'-triphosphate—0.045—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant K384AHomo sapiensaminopterin0.670.01161 calc.pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant K384AHomo sapiensglutamic acid—0.43—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant R377AHomo sapiensadenosine 5'-triphosphate—0.0046—pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant R377AHomo sapiensaminopterin0.0250.00594.2 calc.pH 8.9, 37 °COpen
folylpoly-γ-glutamate synthetase, cytosolic form (rhcFPGS)variant R377AHomo sapiensglutamic acid—350—pH 8.9, 37 °COpen